Targeted gene replacement in fungi using a split-marker approach

Methods in Molecular Biology

Volumn 835, Issue , 2012, Pages 255-269

  Goswami, Rubella S ^a  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

Fungal transformation; Fungi; Fusarium graminearum; Gene deletion; Gene replacement; Gibberella zeae; Split marker

Indexed keywords

FUNGAL DNA;

ARTICLE; FUNGAL GENE; FUNGUS; GENE TARGETING; GENETIC TRANSFORMATION; GENETICS; HOMOLOGOUS RECOMBINATION; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; DNA, FUNGAL; FUNGI; GENE TARGETING; GENES, FUNGAL; HOMOLOGOUS RECOMBINATION; POLYMERASE CHAIN REACTION; TRANSFORMATION, GENETIC;

FUNGI; GIBBERELLA; GIBBERELLA ZEAE;

EID: 84855888272     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-501-5_16     Document Type: Article

References (9)

1. Fairhead C et al (1996) New vectors for combinatorial deletions in yeast chromosomes and for gap-repair cloning using 'split-marker' recombination. Yeast 12: 1439-1457. (Pubitemid 26393225)
2. Fairhead C et al (1998) 'Mass-murder' of ORFs from three regions of chromosome XI from S accharomyces cerevisiae . Gene 223: 33-46. (Pubitemid 29015911)
3. Catlett N et al (2003) Split-marker recombination for effi cient targeted deletion of fungal genes. Fungal Genet . Newsl 50: 9-11.
4. Goswami RS et al (2006). Genomic analysis of host-pathogen inaction during early stages of disease development. Microbiology 152: 1877-1890.
5. Correll JC et al (1987) Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77: 1640-1646.
6. Evans CK et al (2000) Biosynthesis of deoxynivalenol in spikelets of barley inoculated with macroconidia of Fusarium graminearum . Plant Dis 84: 654-660. (Pubitemid 30322860)
7. Gale LR et al (2011) Nivalenol-type populations of Fusarium graminearum and F. asiaticum are prevalent on wheat in southern Louisiana. Phytopathology 101: 124-134.
8. Ho SN et al (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59. (Pubitemid 19125653)
9. Rosewich UL et al (1999) Population genetic analysis corroborates dispersal of Fusarium oxysporum f. sp. radicislycopersici from Florida to Europe. Phytopathology 89: 623-630. (Pubitemid 29373190)