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Volumn 831, Issue , 2012, Pages 19-36

Isotopic labeling of heterologous proteins in the yeast pichia pastoris and kluyveromyces lactis

Author keywords

Fed batch fermentation; Kluyveromyces lactis; NMR; Pichia pastoris; Stable isotope labeling; Yeast expression systems

Indexed keywords

CARBON; DEUTERIUM; NITROGEN; RECOMBINANT PROTEIN;

EID: 84855883019     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-480-3_2     Document Type: Article
Times cited : (27)

References (41)
  • 1
    • 14744299579 scopus 로고
    • Recent advances in the expression of foreign genes in Pichia pastoris
    • DOI 10.1038/nbt0893-905
    • Cregg, J. M., Vedvick, T. S., and Raschke, W. C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris . Biotechnology 11 , 905-910. (Pubitemid 23218978)
    • (1993) Bio/Technology , vol.11 , Issue.8 , pp. 905-910
    • Cregg, J.M.1    Vedvick, T.S.2    Raschke, W.C.3
  • 2
    • 4644304230 scopus 로고    scopus 로고
    • Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris
    • (Downing, A. K., Ed.), Humana Press, Totowa, NJ
    • Pickford, A. R., and O'Leary, J. M. (2008) Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris , in Methods in Molecular Biology (Downing, A. K., Ed.), vol. 278, pp. 17-33, Humana Press, Totowa, NJ.
    • (2008) Methods in Molecular Biology , vol.278 , pp. 17-33
    • Pickford, A.R.1    O'leary, J.M.2
  • 3
    • 0036669602 scopus 로고    scopus 로고
    • Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris
    • DOI 10.1016/S0958-1669(02)00330-0
    • Lin Cereghino, G. P., Cereghino, J. L., Ilgen, C., and Cregg, J. M. (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris . Curr. Opin. Biotechnol. 13 , 329-332. (Pubitemid 35254090)
    • (2002) Current Opinion in Biotechnology , vol.13 , Issue.4 , pp. 329-332
    • Cereghino G.P.Lin1    Cereghino, J.L.2    Ilgen, C.3    Cregg, J.M.4
  • 4
    • 14744285206 scopus 로고    scopus 로고
    • Expression of heterologous proteins in Pichia pastoris: A useful experimental tool in protein engineenring and production
    • DOI 10.1002/jmr.687
    • Daley, R., and Hearn, M. T. (2005) Expression of heterologous proteins in Pichia pastoris : a useful experimental tool in protein engineering and production. J. Mol. Recognit . 18 , 119-138. (Pubitemid 40328574)
    • (2005) Journal of Molecular Recognition , vol.18 , Issue.2 , pp. 119-138
    • Daly, R.1    Hearn, M.T.W.2
  • 5
    • 33748915125 scopus 로고    scopus 로고
    • Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
    • Cos, O., Ramón, R., Montesinos, J. L., and Valero F. (2006) Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review. Microbial Cell Factories 5 , 17-36.
    • (2006) Microbial Cell Factories , vol.5 , pp. 17-36
    • Cos, O.1    Ramón, R.2    Montesinos, J.L.3    Valero, F.4
  • 6
    • 77649182408 scopus 로고    scopus 로고
    • Production of isotopically labeled heterologous proteins in non- E. Coli prokaryotic cells
    • Takahashi, H., and Shimada, I. (2010) Production of isotopically labeled heterologous proteins in non- E. coli prokaryotic cells. J. Biomol. NMR 46 , 3-10.
    • (2010) J. Biomol. NMR , vol.46 , pp. 3-10
    • Takahashi, H.1    Shimada, I.2
  • 7
    • 84873070131 scopus 로고    scopus 로고
    • pPICZ and pPICZα
    • Invitrogen Corporation
    • pPICZ and pPICZα in Pichia pastoris , Version I. Available at http://tools.invitrogen.com/content/sfs/manuals/easyselect-man.pdf .
    • Pichia Pastoris Version i
  • 8
    • 0028081167 scopus 로고
    • Highlevel secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris
    • Laroche, Y., Strome, V., De Meutter, J., Messens, J., and Lauwereys, M. (1994) Highlevel secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris . Bio/Technol 12 , 1119-1124.
    • (1994) Bio/Technol , vol.12 , pp. 1119-1124
    • Laroche, Y.1    Strome, V.2    De Meutter, J.3    Messens, J.4    Lauwereys, M.5
  • 9
    • 0032189230 scopus 로고    scopus 로고
    • Isotopically labeled bovine β-lactoglobulin for NMR studies expressed in Pichia pastoris
    • DOI 10.1006/prep.1998.0924
    • Denton, H., Smith, M., Husi, H., Uhrin, D., Barlow, P. N., Batt, C. A. , and Sawyer, L. (1998) Isotopically labeled bovine β -lactoglobulin for NMR studies expressed in Pichia pastoris . Protein Expr. Purif. 14 , 97-103. (Pubitemid 28476585)
    • (1998) Protein Expression and Purification , vol.14 , Issue.1 , pp. 97-103
    • Denton, H.1    Smith, M.2    Husi, H.3    Uhrin, D.4    Barlow, P.N.5    Batt, C.A.6    Sawyer, L.7
  • 10
    • 0032587512 scopus 로고    scopus 로고
    • Production of large quantifies of isotopically labeled protein in Pichia pastoris by fermentation
    • DOI 10.1023/A:1008398313350
    • Wood, M. J., and Komives, E. A. (1999) Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation.. J. Biomol. NMR 13 , 149-159. (Pubitemid 29089225)
    • (1999) Journal of Biomolecular NMR , vol.13 , Issue.2 , pp. 149-159
    • Wood, M.J.1    Komives, E.A.2
  • 11
    • 0032991055 scopus 로고    scopus 로고
    • 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements
    • DOI 10.1016/S0014-5793(99)00332-4, PII S0014579399003324
    • 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements. FEBS Lett. 448 , 33-37. (Pubitemid 29151625)
    • (1999) FEBS Letters , vol.448 , Issue.1 , pp. 33-37
    • Mine, S.1    Ueda, T.2    Hashimoto, Y.3    Tanaka, Y.4    Imoto, T.5
  • 13
    • 0034923785 scopus 로고    scopus 로고
    • 13C isotopic labeling of proteins expressed in Pichia pastoris
    • 15 N isotopic labeling of proteins expressed in Pichia pastoris . J. Biochem. 130 , 19-22. (Pubitemid 32674560)
    • (2001) Journal of Biochemistry , vol.130 , Issue.1 , pp. 19-22
    • Rodriguez, E.1    Krishna, N.R.2
  • 14
    • 0033816837 scopus 로고    scopus 로고
    • Expression of deuterium-isotope-labelled protein in the yeast Pichia pastoris for NMR study
    • Morgan, W. D., Kragt, A., and Feeney, J. (2000) Expression of deuterium-isotope-labelled protein in the yeast Pichia pastoris for NMR study. J. Biomol. NMR 17 , 337-347.
    • (2000) J. Biomol. NMR , vol.17 , pp. 337-347
    • Morgan, W.D.1    Kragt, A.2    Feeney, J.3
  • 15
    • 34648819975 scopus 로고    scopus 로고
    • Structural basis of the collagen-binding mode of discoidin domain receptor 2
    • DOI 10.1038/sj.emboj.7601833, PII 7601833
    • Ichikawa, M., Osawa, M., Nishida, N., Goshima, N., Nomura, N., and Shimada, I. (2007) Structural basis of the collagen-binding mode of discoidin domain receptor 2, EMBO J . 26 , 4168-4176. (Pubitemid 47462092)
    • (2007) EMBO Journal , vol.26 , Issue.18 , pp. 4168-4176
    • Ichikawa, O.1    Osawa, M.2    Nishida, N.3    Goshima, N.4    Nomura, N.5    Shimada, I.6
  • 17
    • 32044463134 scopus 로고    scopus 로고
    • Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis
    • DOI 10.1128/AEM.71.11.7092-7098.2005
    • Colussi, P. A., and Taron, C. H. (2005) Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis , Appl. Environ. Microbiol . 71 , 7092-7098. (Pubitemid 43194199)
    • (2005) Applied and Environmental Microbiology , vol.71 , Issue.11 , pp. 7092-7098
    • Colussi, P.A.1    Taron, C.H.2
  • 18
    • 34548254386 scopus 로고    scopus 로고
    • Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high-frequency formation of multicopy strains
    • DOI 10.1128/AEM.02253-06
    • Read, J. D., Colussi, P. A., Ganatra, M. B., and Taron, C. H. (2007) Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high-frequency formation of multicopy strains. Appl. Environ. Microbiol . 73 , 5088-5096. (Pubitemid 47326637)
    • (2007) Applied and Environmental Microbiology , vol.73 , Issue.16 , pp. 5088-5096
    • Read, J.D.1    Colussi, P.A.2    Ganatra, M.B.3    Taron, C.H.4
  • 19
    • 55649087551 scopus 로고    scopus 로고
    • Stable isotope labeling of protein by Kluyveromyces lactis for NMR study
    • Sugiki, T., Shimada, I., and Takahashi, H. (2008) Stable isotope labeling of protein by Kluyveromyces lactis for NMR study. J. Biomol. NMR 42 , 159-162.
    • (2008) J. Biomol. NMR , vol.42 , pp. 159-162
    • Sugiki, T.1    Shimada, I.2    Takahashi, H.3
  • 21
    • 0034051065 scopus 로고    scopus 로고
    • A novel NMR method for determining the interfaces of large protein- protein complexes
    • DOI 10.1038/73331
    • Takahashi, H., Nakanishi, T., Kami, K., Arata, Y., and Shimada, I. (2000) A novel NMR method for determining the interface of large protein-protein complexes. Nat. Struct. Biol . 7 , 220-223. (Pubitemid 30140768)
    • (2000) Nature Structural Biology , vol.7 , Issue.3 , pp. 220-223
    • Takahashi, H.1    Nakanishi, T.2    Kami, K.3    Arata, Y.4    Shimada, I.5
  • 22
    • 0036302354 scopus 로고    scopus 로고
    • Determination of the interface of a large protein complex by transferred cross-saturation measurements
    • DOI 10.1016/S0022-2836(02)00018-9
    • Nakanishi, T., Miyazawa, M., Sakakura, M., Terasawa, H., Takahashi, H., and Shimada, I. (2002) Determination of the interface of a large protein complex by transferred cross-saturation measurements. J. Mol. Biol . 318 , 245-249. (Pubitemid 34734823)
    • (2002) Journal of Molecular Biology , vol.318 , Issue.2 , pp. 245-249
    • Nakanishi, T.1    Miyazawa, M.2    Sakakura, M.3    Terasawa, H.4    Takahashi, H.5    Shimada, I.6
  • 23
    • 33645785076 scopus 로고    scopus 로고
    • Utilization of methyl proton resonances in cross-saturation measurement for determining the interfaces of large protein-protein complexes
    • Takahashi, H., Miyazawa, M., Ina, Y., Fukunishi, Y., Mizukoshi, Y., Nakamura, H., and Shimada, I. (2006) Utilization of methyl proton resonances in cross-saturation measurement for determining the interfaces of large protein-protein complexes. J. Biomol. NMR 34 , 167-177.
    • (2006) J. Biomol. NMR , vol.34 , pp. 167-177
    • Takahashi, H.1    Miyazawa, M.2    Ina, Y.3    Fukunishi, Y.4    Mizukoshi, Y.5    Nakamura, H.6    Shimada, I.7
  • 25
    • 0029140698 scopus 로고
    • Effect of antifoam agents on the medium and microbial cell properties and process performance in small and large reactors
    • Koch, V., Rüffer, H.-M., Schügerl, K., Innertsberger, E., Menzel, H., and Weis, J. (1995) Effect of antifoam agents on the medium and microbial cell properties and process performance in small and large reactors. Process Biochem . 30 , 435-446.
    • (1995) Process Biochem , vol.30 , pp. 435-446
    • Koch, V.1    Rüffer, H.-M.2    Schügerl, K.3    Innertsberger, E.4    Menzel, H.5    Weis, J.6
  • 26
    • 17244363998 scopus 로고    scopus 로고
    • Heterologous protein production using the Pichia pastoris expression system
    • DOI 10.1002/yea.1208
    • Macauley-Patrick, S., Fazenda, M. L., McNeil, B., and Harvey, L. M. (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22 , 249-270. (Pubitemid 40528349)
    • (2005) Yeast , vol.22 , Issue.4 , pp. 249-270
    • Macauley-Patrick, S.1    Fazenda, M.L.2    McNeil, B.3    Harvey, L.M.4
  • 27
    • 0038128640 scopus 로고    scopus 로고
    • Expression of Sonic hedgehog-Fc fusion protein in Pichia pastoris. Identification and control of post-translational, chemical, and proteolytic modifications
    • Shapiro, R. I., Wen, D., Levesque, M., Hronowski, X., Gill, A., Garber, E. A., Galdes, A., Strauch, K. L., and Taylor, F. R. (2003) Expression of sonic hedgehog-Fc fusion protein in Pichia pastoris . Identification and control of post-translational, chemical, and proteolytic modifications. Protein Expr. Purif . 29 , 272-283. (Pubitemid 36720260)
    • (2003) Protein Expression and Purification , vol.29 , Issue.2 , pp. 272-283
    • Shapiro, R.I.1    Wen, D.2    Levesque, M.3    Hronowski, X.4    Gill, A.5    Garber, E.A.6    Galdes, A.7    Strauch, K.L.8    Taylor, F.R.9
  • 28
    • 0035807422 scopus 로고    scopus 로고
    • A Pichia pastoris fermentation process for producing high-levels of recombinant human cystatin-C
    • DOI 10.1016/S0141-0229(01)00395-7, PII S0141022901003957
    • Files, D., Ogawa, M., Scaman, C. H., and Baldwin, S. A. (2001) A Pichia pastoris fermentation process for producing high-levels of recombinant human cystatin-C. Enzyme Microbial Technol . 29 , 335-340. (Pubitemid 32925153)
    • (2001) Enzyme and Microbial Technology , vol.29 , Issue.6-7 , pp. 335-340
    • Files, D.1    Ogawa, M.2    Scaman, C.H.3    Baldwin, S.A.4
  • 30
    • 0037275711 scopus 로고    scopus 로고
    • Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources
    • DOI 10.1023/A:1021905010021
    • Xie, J., Zhang, L., Ye, Q., Zhou, Q., Xin, L., Du, P., and Gan, R. (2003) Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources. Biotechnol. Lett . 25 , 173-177. (Pubitemid 36189365)
    • (2003) Biotechnology Letters , vol.25 , Issue.2 , pp. 173-177
    • Xie, J.1    Zhang, L.2    Ye, Q.3    Zhou, Q.4    Xin, L.5    Du, P.6    Gan, R.7
  • 31
    • 0030974549 scopus 로고    scopus 로고
    • Expression of trimeric CD40 ligand in Pichia pastoris: Use of a rapid method to detect high-level expressing transformants
    • DOI 10.1016/S0378-1119(96)00747-0, PII S0378111996007470
    • McGrew, J. T., Leiske, D., Dell, B., Klinke, R., Krasts, D., Wee, S. F., Abbott, N., Armitage, R., and Harrington, K. (1997) Expression of trimeric CD40 ligand in Pichia pastoris : use of a rapid method to detect high-level expressing transformants. Gene 187 , 193-200. (Pubitemid 27140248)
    • (1997) Gene , vol.187 , Issue.2 , pp. 193-200
    • McGrew, J.T.1    Leiske, D.2    Dell, B.3    Klinke, R.4    Krasts, D.5    Wee, S.6    Abbott, N.7    Armitage, R.8    Harrington, K.9
  • 32
    • 0035808160 scopus 로고    scopus 로고
    • Rational approach to improving productivity in recombinant Pichia pastoris fermentation
    • DOI 10.1002/1097-0290(20010105)72:1<1::AID-BIT1>3.0.CO;2-T
    • d'Anjou, M. C., and Daugulis, A. J. (2001) A rational approach to improving productivity in Pichia pastoris fermentation. Biotechnol. Bioeng 72 , 1-11. (Pubitemid 32085886)
    • (2001) Biotechnology and Bioengineering , vol.72 , Issue.1 , pp. 1-11
    • D'Anjou, M.C.1    Daugulis, A.J.2
  • 33
    • 0031627465 scopus 로고    scopus 로고
    • An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli
    • Cai, M., Huang, Y., Sakaguchi, K., Clore, G. M., Gronenborn, A. M., and Craigie, R. (1998) An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli . J. Biomol. NMR 11 , 97-102. (Pubitemid 128510229)
    • (1998) Journal of Biomolecular NMR , vol.11 , Issue.1 , pp. 97-102
    • Cai, M.1    Huang, Y.2    Sakaguchi, K.3    Clore, G.M.4    Gronenborn, A.M.5    Craigie, R.6
  • 34
    • 38849181132 scopus 로고    scopus 로고
    • The proteolytic systems and heterologous proteins degradation in the methylotrophic yeast Pichia pastoris
    • Zhang, Y., Liu, R., and Wu, X. (2007) The proteolytic systems and heterologous proteins degradation in the methylotrophic yeast Pichia pastoris . Annal. Microbiol . 57 , 553-560. (Pubitemid 351196967)
    • (2007) Annals of Microbiology , vol.57 , Issue.4 , pp. 553-560
    • Zhang, Y.1    Liu, R.2    Wu, X.3
  • 35
    • 0035715001 scopus 로고    scopus 로고
    • Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris
    • DOI 10.1006/prep.2001.1395
    • Li, Z., Xiong, F., Lin, Q., d'Anjou, M., Daugulis, A. J., Yang, D. S., and Hew, C. L. (2001) Lowtemperature increases the yield of biological active herring antifreeze protein in Pichia pastoris . Protein Expr. Purif . 21 , 438-445. (Pubitemid 34179417)
    • (2001) Protein Expression and Purification , vol.21 , Issue.3 , pp. 438-445
    • Li, Z.1    Xiong, F.2    Lin, Q.3    D'Anjou, M.4    Daugulis, A.J.5    Yang, D.S.C.6    Hew, C.L.7
  • 36
    • 0037430841 scopus 로고    scopus 로고
    • Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes
    • DOI 10.1016/S0168-1656(03)00003-8
    • Jahic, M., Gustavsson, M., Jansen, A.-K., Martinelle, M., and Enfors, S.-O. (2003) Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J. Biotechnol . 102 , 45-53. (Pubitemid 36369202)
    • (2003) Journal of Biotechnology , vol.102 , Issue.1 , pp. 45-53
    • Jahic, M.1    Gustavsson, M.2    Jansen, A.-K.3    Martinelle, M.4    Enfors, S.-O.5
  • 37
    • 2942627936 scopus 로고    scopus 로고
    • Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
    • Jahic, M., Wallberg, F., Bollok, M., Garcia, P., and Enfors, S.-O. (2003) Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microbial Cell Factories 2 , 6-17.
    • (2003) Microbial Cell Factories , vol.2 , pp. 6-17
    • Jahic, M.1    Wallberg, F.2    Bollok, M.3    Garcia, P.4    Enfors, S.-O.5
  • 38
    • 34250333764 scopus 로고    scopus 로고
    • Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
    • DOI 10.1016/j.jbiotec.2007.04.009, PII S0168165607003197
    • Surribas, A., Stahn, R., Montesinos, J. L., Enfors, S.-O., Valero, F., and Jahic, M. (2007) Production of Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies. J. Biotechnol . 130 , 291-299. (Pubitemid 46920541)
    • (2007) Journal of Biotechnology , vol.130 , Issue.3 , pp. 291-299
    • Surribas, A.1    Stahn, R.2    Montesinos, J.L.3    Enfors, S.-O.4    Valero, F.5    Jahic, M.6
  • 39
    • 0038057544 scopus 로고    scopus 로고
    • Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris
    • DOI 10.1016/S1046-5928(02)00706-4
    • Shi, X., Karkut T., Chamankhah, M., Alting- Mees, M., Hemmingsen, S. M., and Hegedus, D. (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris . Protein Expr. Purif. 28 , 321-330. (Pubitemid 36562423)
    • (2003) Protein Expression and Purification , vol.28 , Issue.2 , pp. 321-330
    • Shi, X.1    Karkut, T.2    Chamankhah, M.3    Alting-Mees, M.4    Hemmingsen, S.M.5    Hegedus, D.6
  • 40
    • 57949087782 scopus 로고    scopus 로고
    • Degradation of HSAAX15(R13K) when expressed in Pichia pastoris can be reduced via the disruption of YPS1 gene in this yeast
    • Yao, X. Q., Zhao, H. L., Xue, C., Zhang, W., Xiong, X. H., Wang, Z. W., Li, X. Y., and Liu, Z. M. (2009) Degradation of HSAAX15(R13K) when expressed in Pichia pastoris can be reduced via the disruption of YPS1 gene in this yeast. J. Biotechnol. 139 , 131-136.
    • (2009) J. Biotechnol , vol.139 , pp. 131-136
    • Yao, X.Q.1    Zhao, H.L.2    Xue, C.3    Zhang, W.4    Xiong, X.H.5    Wang, Z.W.6    Li, X.Y.7    Liu, Z.M.8
  • 41
    • 60349115855 scopus 로고    scopus 로고
    • Development of stable isotope and selenomethionine labeling methods for proteins expressed in Pseudomonas fl uorescens
    • Madduri, K., Badger, M., Li, Z.-S., Xu, X., Thornburgh, S., Evans, S., and Dhadialla, T. S. (2009) Development of stable isotope and selenomethionine labeling methods for proteins expressed in Pseudomonas fl uorescens . Protein Expr. Purif . 65 , 57-65.
    • (2009) Protein Expr. Purif , vol.65 , pp. 57-65
    • Madduri, K.1    Badger, M.2    Li, Z.-S.3    Xu, X.4    Thornburgh, S.5    Evans, S.6    Dhadialla, T.S.7


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