An efficient purification system for native minichromosome from saccharomyces cerevisiae

Methods in Molecular Biology

Volumn 833, Issue , 2012, Pages 115-123

  Unnikrishnan, Ashwin ^a   Akiyoshi, Bungo ^a   Biggins, Sue ^a   Tsukiyama, Toshio ^a  

^a USA   (United States)

Author keywords

Chromatin; DNA replication; Episome; FLAG tag; Histone modifications; Mass spectrometry; Minichromosome; Purification; Replication origin; TRP1 ARS1

Indexed keywords

FUNGUS ANTIBODY; MICROSPHERE; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CHROMOSOME; CHROMOSOME MAP; GENETICS; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; MAGNETISM; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE;

ANTIBODIES, FUNGAL; CHROMOSOME MAPPING; CHROMOSOMES, FUNGAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MAGNETICS; MICROSPHERES; MOLECULAR BIOLOGY; PLASMIDS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 84855882412     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-477-3_8     Document Type: Article

References (5)

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2. Unnikrishnan, A., Gafken, P. R., and Tsukiyama, T. (2010) Dynamic changes in histone acetylation regulate origins of DNA replication, Nat Struct Mol Biol 17, 430-437.
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5. Akiyoshi, B., Nelson, C. R., Ranish, J. A., and Biggins, S. (2009) Quantitative proteomic analysis of purifi ed yeast kinetochores identifi es a PP1 regulatory subunit, Genes Dev 23, 2887-2899.