Ammonia-containing dimethyl sulfoxide: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay

Analytical Biochemistry

Volumn 421, Issue 1, 2012, Pages 324-326

  Wang, Hengwei ^a   Wang, Fengqing ^b   Tao, Xinyi ^b   Cheng, Hairong ^a  

^a Shanghai Jiao Tong University   (China)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Ammonia; Dimethyl sulfoxide; Formazan; MTT assay; pH; Viability

Indexed keywords

ALKALINITY; AMMONIA; CRYSTALS; DISSOLUTION; ORGANIC SOLVENTS; PH; SODIUM HYDROXIDE;

3-(4 ,5-DIMETHYLTHIAZOL-2-YL)-2 ,5-DIPHENYL TETRAZOLIUM BROMIDE; A-STABLE; BUFFERING EFFECT; DIMETHYL SULFOXIDE (DMSO); FORMAZAN; MTT ASSAYS; VIABILITY;

DIMETHYL SULFOXIDE;

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; AMMONIA; BUFFER; DIMETHYL SULFOXIDE; FORMAZAN; HYDROCHLORIC ACID; SODIUM HYDROXIDE; SOLVENT;

ARTICLE; COLORIMETRY; CRYSTAL; DISSOLUTION; PH; PRIORITY JOURNAL;

EID: 84855875187     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.10.043     Document Type: Article

References (14)

1. J. Gabrielson, M. Hart, A. Jarelov, I. Kuhn, D. McKenzie, and R. Mollby Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates J. Microbiol. Methods 50 2002 63 73 (Pubitemid 34270790)
2. M. Rahman, I. Kühn, M. Rahman, B. Olsson-Liljequist, and R. Möllby Evaluation of a scanner-assisted colorimetric MIC method for susceptibility testing of gram-negative fermentative bacteria Appl. Environ. Microbiol. 70 2004 2398 2403 (Pubitemid 38500544)
3. C. Stentelaire, N. Antoine, C. Cabrol, G. Feron, and A. Durand Development of a rapid and highly sensitive biochemical method for the measurement of fungal spore viability: an alternative to the CFU method Enzyme Microb. Technol. 29 2001 560 566 (Pubitemid 32964797)
4. M.M. Tunney, G. Ramage, T.R. Field, T.F. Moriarty, and D.G. Storey Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa Antimicrob. Agents Chemother. 48 2004 1879 1881 (Pubitemid 38544403)
5. J. Carmichael, W.G. DeGraff, A.F. Gazdar, J.D. Minna, and J.B. Mitchell Evaluation of a tetrazolium-based semi-automated colorimetric assay: assessment of chemosensitivity testing Cancer Res. 47 1987 936 942 (Pubitemid 17027905)
6. D.M. Morgan Tetrazolium (MTT) assay for cellular viability and activity Methods Mol. Biol. 79 1998 179 183
7. T. Mosman Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays J. Immunol. Methods 65 1983 55 63
8. H.W. Wang, H.R. Cheng, F.Q. Wang, D.Z. Wei, and X.D. Wang An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells J. Microbiol. Methods 82 2010 330 333
9. R. Chakrabarti, S. Kundu, S. Kumar, and R. Chakrabarti Vitamin A as an enzyme that catalyzes the reduction of MTT to formazan by vitamin C J. Cell Biochem. 80 2000 133 138
10. L. Peng, B. Wang, and P. Ren Reduction of MTT by flavonoids in the absence of cells Colloids Surf. B 45 2005 108 111 (Pubitemid 41266927)
11. T.P. Talorete, M. Bouaziz, S. Sayadi, and H. Isoda Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells Cytotechnology 52 2006 189 198 (Pubitemid 46686122)
12. M. Pozzolini, S. Scarfi, U. Benatti, and M. Giovine Interference in MTT cell viability assay in activated macrophage cell line Anal. Biochem. 313 2003 338 341 (Pubitemid 36259961)
13. C. Rollino, S. Borsa, G. Bellone, G. Piccoli, and G. Emanuelli False positive results with MTT assay J. Immunol. Methods 185 1995 141 143
14. J.A. Plumb, R. Milroy, and S.B. Kaye Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide-formazan absorption on chemosensitivity determined by a novel tetrazolium-based assay Cancer Res. 49 1989 4435 4440 (Pubitemid 19208340)