Detection of hepatitis C virus subtypes 6a, 6n, 6w and mixed infections using a modified multiplex real-time polymerase chain reaction protocol

Journal of the Formosan Medical Association

Volumn 110, Issue 12, 2011, Pages 762-767

  Lee, Yuan Ming ^a,b   Chen, Yen Ju ^a   Lee, Cheng Ming ^a   Kuo, Lou Hui ^a   Wong, Wing Wai ^a,c   Arthur Chen, Yi Ming ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b SAINT PAUL S HOSPITAL   (Taiwan)

^c TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

Genotype 6; Hepatitis C virus (HCV); Injection drug users; Inno LiPa II test; Multiplex RT PCR; Subtype 6n; Subtype 6w

Indexed keywords

ARTICLE; CONTROLLED STUDY; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; MIXED INFECTION; MULTIPLEX POLYMERASE CHAIN REACTION; PHYLOGENETIC TREE; REAL TIME POLYMERASE CHAIN REACTION; TAIWAN; VIRUS DETECTION;

GENOTYPING TECHNIQUES; HEPACIVIRUS; HEPATITIS C; HUMANS; PHYLOGENY; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84855830452     PISSN: None     EISSN: 09296646     Source Type: Journal    
DOI: 10.1016/j.jfma.2011.11.006     Document Type: Article

References (15)

1. Simmonds P. Genetic diversity and evolution of hepatitis C virus-15 years on. J Gen Virol 2004, 85:3173-3188.
2. Zein N.N. Clinical significance of hepatitis C virus genotypes. Clin Microbiol Rev 2000, 13:223-235.
3. Stuyver L., Wyseur A., van A.W., Hernandez F., Maertens G. Second-generation line probe assay for hepatitis C virus genotyping. JClin Microbiol 1996, 34:2259-2266.
4. Nakano I., Fukuda Y., Katano Y., Toyoda H., Hayashi K., Hayakawa T., et al. Interferon responsiveness in patients infected with hepatitis C virus 1b differs depending on viral subtype. Gut 2001, 49:263-267.
5. Zein N.N., Rakela J., Krawitt E.L., Reddy K.R., Tominaga T., Persing D.H. Hepatitis C virus genotypes in the United States: epidemiology, pathogenicity, and response to interferon therapy. Collaborative Study Group. Ann Intern Med 1996, 125:634-639.
6. Nakao T., Enomoto N., Takada N., Takada A., Date T. Typing of hepatitis C virus genomes by restriction fragment length polymorphism. J Gen Virol 1991, 72:2105-2112.
7. McOmish F., Yap P.L., Dow B.C., Follett E.A., Seed C., Keller A.J., et al. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. J Clin Microbiol 1994, 32:884-892.
8. Chayama K., Kobayashi M., Tsubota A., Koida I., Arase Y., Saitoh S., et al. Molecular analysis of intraspousal transmission of hepatitis C virus. J Hepatol 1995, 22:431-439.
9. Okamoto H., Sugiyama Y., Okada S., Kurai K., Akahane Y., Sugai Y., et al. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol 1992, 73:673-679.
10. Lee Y.M., Lin H.J., Chen Y.J., Lee C.M., Wang S.F., Chang K.Y., et al. Molecular epidemiology of HCV genotypes among injection drug users in Taiwan: full-length sequences of two new subtype 6w strains and a recombinant form_2b6w. J Med Virol 2010, 82:57-68.
11. Ohno O., Mizokami M., Wu R.R., Saleh M.G., Ohba K., Orito E., et al. New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a. J Clin Microbiol 1997, 35:201-207.
12. Retief J.D. Phylogenetic analysis using PHYLIP. Methods Mol Biol 2000, 132:243-258.
13. Kumar S., Tamura K., Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 2004, 5:150-163.
14. Zhou D.X., Tang J.W., Chu I.M., Cheung J.L., Tang N.L., Tam J.S., et al. Hepatitis C virus genotype distribution among intravenous drug user and the general population in Hong Kong. J Med Virol 2006, 78:574-581.
15. Liu J.Y., Lin H.H., Liu Y.C., Lee S.S., Chen Y.L., Hung C.C., et al. Extremely high prevalence and genetic diversity of hepatitis C virus infection among HIV-infected injection drug users in Taiwan. Clin Infect Dis 2008, 46:1761-1768.