Integration of gene-based markers in a pearl millet genetic map for identification of candidate genes underlying drought tolerance quantitative trait loci

BMC Plant Biology

Volumn 12, Issue , 2012, Pages

  Sehgal, Deepmala ^a   Rajaram, Vengaldas ^b   Armstead, Ian P ^a   Vadez, Vincent ^b   Yadav, Yash P ^c   Hash, Charles T ^b   Yadav, Rattan S ^a  

^a ABERYSTWYTH UNIVERSITY   (United Kingdom)

^b INTERNATIONAL CROPS RESEARCH INSTITUTE FOR THE SEMI ARID TROPICS ICRISAT   (India)

^c CCS HARYANA AGRICULTURAL UNIVERSITY   (India)

Author keywords

Candidate genes; CISP; Drought tolerance QTLs; EST SSR; Pearl millet; SNP

Indexed keywords

PENNISETUM GLAUCUM; POACEAE;

PLANT DNA;

ARTICLE; CHROMOSOME MAP; DROUGHT; EXPRESSED SEQUENCE TAG; GENE FREQUENCY; GENETIC MARKER; GENETICS; GENOTYPE; PENNISETUM; PHYSIOLOGICAL STRESS; PHYSIOLOGY; QUANTITATIVE TRAIT LOCUS; SINGLE NUCLEOTIDE POLYMORPHISM;

CHROMOSOME MAPPING; DNA, PLANT; DROUGHTS; EXPRESSED SEQUENCE TAGS; GENE FREQUENCY; GENETIC MARKERS; GENOTYPE; PENNISETUM; POLYMORPHISM, SINGLE NUCLEOTIDE; QUANTITATIVE TRAIT LOCI; STRESS, PHYSIOLOGICAL;

EID: 84855820182     PISSN: None     EISSN: 14712229     Source Type: Journal    
DOI: 10.1186/1471-2229-12-9     Document Type: Article

References (86)

1. Liu CJ, Witcombe JR, Pittaway TS, Nash M, Hash CT, Busso CS, Gale MD. An RFLP-based genetic map of pearl millet (Pennisetum glaucum). Theor Appl Genet 1994, 89:481-487.
2. Devos KM, Gale MD. Comparative genetics in the grasses. Plant Mol Biol 1997, 35:3-15. 10.1023/A:1005820229043, 9291955.
3. Devos KM, Pittaway TS, Reynolds A, Gale MD. Comparative mapping reveals a complex relationship between the pearl millet genome and those of foxtail millet and rice. Theor Appl Genet 2000, 100:190-198.
4. Yadav RS, Hash CT, Bidinger FR, Cavan GP, Howarth CJ. Quantitative trait loci associated with traits determining grain and stover yield in pearl millet under terminal drought stress conditions. Theor Appl Genet 2002, 104:67-83. 10.1007/s001220200008, 12579430.
5. Yadav RS, Hash CT, Bidinger FR, Devos KM, Howarth CJ. Genomic regions associated with grain yield and aspects of post-flowering drought tolerance in pearl millet across stress environments and tester background. Euphytica 2004, 136:265-277.
6. Qi X, Pittaway TS, Lindup S, Liu H, Waterman E, Padi FK, Hash CT, Zhu J, Gale MD, Devos KM. An integrated genetic map and a new set of simple sequence repeat markers for pearl millet Pennisetum glaucum. Theor Appl Genet 2004, 109:1485-1493. 10.1007/s00122-004-1765-y, 15322756.
7. Senthilvel S, Jayashree B, Mahalakshmi V, Sathish Kumar P, Nakka S, Nepolean T, Hash CT. Development and mapping of simple sequence repeat markers for pearl millet from data mining of expressed sequence tags. BMC Plant Biol 2008, 8:119. 10.1186/1471-2229-8-119, 2632669, 19038016.
8. Supriya, Senthilvel S, Nepolean T, Eshwar K, Rajaram V, Shaw R, Hash CT, Kilian A, Yadav RC, Narasu ML. Development of a molecular linkage map of pearl millet integrating DArT and SSR markers. Theor Appl Genet 2011, doi:101007/s00122-011-1580-1.
9. Bidinger FR, Nepolean T, Hash CT, Yadav RS, Howarth CJ. Identification of QTLs for grain yield of pearl millet [Pennisetum glaucum (L.) R Br] in environments with variable moisture during grain filling. Crop Sci 2007, 47:969-980.
10. Jones ES, Liu CJ, Gale MD, Hash CT, Witcombe JR. Mapping quantitative trait loci for downy mildew resistance in pearl millet. Theor Appl Genet 1995, 91:448-456.
11. Jones ES, Breese WA, Liu CJ, Singh SD, Shaw DS, Witcombe JR. Mapping quantitative trait loci for resistance to downy mildew in pearl millet: Field and glasshouse screens detect the same QTL. Crop Sci 2002, 42:1316-1323.
12. Morgan RN, Wilson JP, Hanna WW, Ozais-Akins P. Molecular markers for rust and pyricularia leaf spot disease resistance in pearl millet. Theor Appl Genet 1998, 96:413-420.
13. Breese WA, Hash CT, Devos KM, Howarth CJ. Pearl millet genomics - an overview with respect to breeding for resistance to downy mildew. Sorghum and Millets Pathology 2000, 243-246. Ames Iowa Iowa State Press, Leslie JF.
14. Gulia SK, Hash CT, Thakur RP, Breese WA, Sangwan RS, Singh DP, Tomar VS, Behl RK, Upadhyaya SD, Bhale MS, Khare D. Crop Production in Stress Environments--Genetic and Management Option. Mapping new QTLs for downy mildew Sclerospora graminicol (Sacc) J Schroet] resistance in pearl millet Pennisetum glaucum (L) R Br 2007, 373-386. Jodhpur, India: Agrobios Publishers.
15. Nepolean T, Blümmel M, Bhasker Raj AG, Rajaram V, Senthilvel S, Hash CT. QTLs controlling yield and stover quality traits in pearl millet. Int Sorghum Millets Newsl 2006, 47:149-152.
16. Allouis S, Qi X, Lindup S, Gale MD, Devos KM. Construction of a BAC library of pearl millet Pennisetum glaucum. Theor Appl Genet 2001, 102:1200-1205.
17. Gebhardt C, Li L, Pajerowska-Mukthar K, Achenbach U, Sattarzadeh A, Bormann C, Ilarionova E, Ballvora A. Candidate gene approach to identify genes underlying quantitative traits and develop diagnostic markers in potato. Crop Sci 2007, 47:S106-S111.
18. Prioul JL, Pelleschi S, Se'ne M, Thévenot C, Causse M, de Vienne D, Leonardi A. From QTLs for enzyme activity to candidate genes in maize. J Exp Bot 1999, 50:1281-1288.
19. Thornsberry JM, Goodman MM, Doebley J, Kresovich S, Nielsen D, Buckler ES. Dwarf8 polymorphisms associate with variation in fowering time. Nat Genet 2001, 28:286-289. 10.1038/90135, 11431702.
20. Sorrells ME, Wilson WA. Direct classification and selection of superior alleles for crop improvement. Crop Sci 1997, 37:691-697.
21. Zhang XQ, Li C, Panozzo J, Westcott S, Zhang G, Tay A, Appels R, Jones M, Lance R. Dissecting the telomeric region of barley chromosome 5HL using rice gene sequences as references: new markers for tracking a complex region in breeding. Mol Breed 2011, 27:1-9.
22. Andersen JR, Lübberstedt T. Functional markers in plants. Trends Plant Sci 2003, 8:554-560. 10.1016/j.tplants.2003.09.010, 14607101.
23. Schneider K, Weisshaar B, Borchardt DC, Salamini F. SNPs frequency and allelic haplotype of Beta vulgaris expressed genes. Mol Breed 2001, 8:63-74.
24. Ching A, Caldwell KS, Jung M, Dolan M, Smith OS, Tingey S, Morgante M, Rafalski AJ. SNP frequency haplotype structure and linkage disequilibrium in elite maize inbred lines. BMC Genet 2002, 3:19. 130040, 12366868.
25. Nasu S, Suzuki J, Ohta R, Hasegawa K, Yui R, Kitazawa N, Monna L, Minobe Y. Search for and analysis of single nucleotidepolymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNPs markers. DNA Res 2002, 9:163-171. 10.1093/dnares/9.5.163, 12465716.
26. Zhu YL, Song QJ, Hyten DL, Van Tassell CP, Matukumalli LK, Grimm DR, Hyatt SM, Fickus EW, Young ND, Cregan PB. Single-nucleotide polymorphisms in soybean. Genetics 2003, 163:1123-1134. 1462490, 12663549.
27. Grivet L, Glaszmann JC, Vincentz M, da Silva F, Arruda P. ESTs as a source for sequence polymorphism discovery in sugarcane: example of the Adh genes. Theor Appl Genet 2003, 106:190-197.
28. Rostoks N, Mudie S, Cardle L, Russell J, Ramsay L, Booth A, Svensson JT, Wanamaker SI, Walia H, Rodriguez EM, Hedley PE, Liu H, Morris J, Close TJ, Marshall DF, Waugh R. Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Mol Genet Genom 2005, 274:515-527.
29. Möhring S, Salamini F, Schneider K. Multiplexed linkage group-specific marker sets for rapid genetic mapping and fingerprinting of sugar beet (Beta vulgaris L.). Mol Breed 2004, 14:475-488.
30. Kota R, Varsheney RK, Prasad M, Zhang H, Stein N, Graner A. EST-derived single nucleotide polymorphism markers for assembling genetic and physical maps of the barley genome. Funct Integr Genom 2008, 8:223-233.
31. Achenbach U, Paulo J, Ilarionova E, Lübeck J, Stahwald J, Tacke E, Hofferbert H-R, Gebhardt C. Using SNP markers to dissect linkage disequilibrium at a major quantitative trait locus for resistance to the potato cyst nematode Globodera pallid on potato chromosome V. Theor Appl Genet 2009, 118:619-629. 10.1007/s00122-008-0925-x, 19020852.
32. Zhang J, Lu Y, Yuan Y, Zhang X, Geng J, Chen Y, Cloutier S, McVetty PBE, Li G. Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa. Plant Mol Biol 2009, 69:553-563. 10.1007/s11103-008-9437-y, 19039665.
33. Kawuki RS, Ferguson M, Labuschagne M, Herselman L, Kim DJ. Identification characterisation and application of single nucleotide polymorphisms for diversity assessment in cassava (Manihot esculenta Crantz). Mol Breed 2009, 23:669-684.
34. Chao S, Zhang W, Akhunov E, Sherman J, Ma Y, Luo M-C, Dubcovsky J. Analysis of gene-derived SNP marker polymorphism in US wheat (Triticum aestivum L.) cultivars. Mol Breed 2009, 23:23-33.
35. Rafalski A. Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol 2002, 5:94-100. 10.1016/S1369-5266(02)00240-6, 11856602.
36. Rajaram V, Varshney RK, Vadez V, Nepolean T, Senthilvel S, Kholová J, Choudhary S, Supriya NO, Kumar S, Thakur RP, Sharma R, Pandurangarao P, Rai KN, Velu G, Sahrawat KL, Bhaskar Raj AG, Blümmel M, Narasu ML, Kocová M, Kavi Kishor PB, Yadav RC, Singh G, Hash CT. Development of EST Resources in pearl millet and their use in development and mapping of EST-SSRs in four RIL populations [abstract. San Diego, California, USA: Plant and Animal Genome Conference 2010, 2010:373.
37. Pflieger S, Lefebvre V, Causse M. The candidate gene approach in plant genetics: a review. Mol Breed 2001, 7:275-291.
38. Zhu M, Zhao S. Candidate Gene Identification Approach: Progress and Challenges. Int J Biol Sci 2007, 3:420-427. ISSN, 2043166, 17998950.
39. Nguyen TT, Klueva N, Chamareck V, Aarti A, Magpantay G, Millena ACM, Pathan MS, Nguyen HT. Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Genet Genom 2004, 272:35-46.
40. Lai Z, Livingstone K, Zou Y, Church SA, Knapp SJ, Andrews J, Rieseberg LH. Identification and mapping of SNPs from ESTs in sunflower. Theor Appl Genet 2005, 111:1532-1544. 10.1007/s00122-005-0082-4, 2442914, 16205907.
41. Schneider K, Kulosa D, Soerensen TR, Möhring S, Heine M, Dursewitz G, Polley A, Weber E. Jamsari, Lein J, Hohmann U, Tahiro E, Weisshaar B, Schulz B, Koch G, Jung C, Ganal M: Analysis of DNA polymorphisms in sugar beet (Beta vulgaris L.) and development of an SNP based map of expressed genes. Theor Appl Genet 2007, 115:601-615. 10.1007/s00122-007-0591-4, 17622508.
42. Tondelli A, Francia E, Barabaschi D, Aprile A, Skinner JS, Stockinger EJ, Stanca AM, Pecchioni N. Mapping regulatory genes for cold and drought stress tolerance in barley. Theor Appl Genet 2006, 112:445-454. 10.1007/s00122-005-0144-7, 16315028.
43. Diab AA, Kantety RV, Ozturk NZ, Benscher D, Nachit MM, Sorells ME. Drought-inducible genes and expressed sequence tags associated with components of drought tolerance in durum wheat. Scien Res Assay 2008, 3:9-26.
44. Bertin I, Zhu JH, Gale MD. SSCP-SNP in pearl millet--a new marker system for comparative genetics. Theor Appl Genet 2005, 110:1467-1472. 10.1007/s00122-005-1981-0, 15809850.
45. Livingstone DS, Motamayor JC, Schnell RJ, Cariaga K, Freeman B, Meerow AW, Brown JS, Kuhn DN. Development of single nucleotide polymorphism markers in Theobroma cacao and comparison to simple sequence repeat markers for genotyping of Cameroon clones. Mol Breed 2011, 27:93-106.
46. Vroh Bi I, McMullen MD, Sanchez-Villeda H, Schroeder S, Gardiner J, Polacco M, Soderlund C, Wing R, Fang Z, Coe EH. Single nucleotide polymorphism and insertion-deletion for genetic markers and anchoring the maize fingerprint contig physical map. Crop Sci 2006, 46:12-21.
47. Cogan NO, Ponting RC, Vecchies AC, Drayton MC, George J, Dracatos PM, Dobrowolski MP, Sawbridge TI, Smith KF, Spangenberg GC. Gene-associated single nucleotide polymorphism discovery in perennial ryegrass (Lolium perenne L.). Mol Genet Genom 2006, 276:101-112.
48. Varsheney RK, Beier U, Khlestkina EK, Kota R, Korzun V, Graner A, Börner A. Single nucleotide polymorphisms in rye (Secale cereale L.): discovery frequency and applications for genome mapping and diversity studies. Theor Appl Genet 2007, 114:1105-1116. 10.1007/s00122-007-0504-6, 17345059.
49. Tenaillon MI, Sawkins MC, Long AD, Gaut B, Doebley JF, Brandon S. Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.). Proc Natl Acad Sci USA 2001, 98:9161-9166. 10.1073/pnas.151244298, 55390, 11470895.
50. Bundock PC, Henry RJ. Single nucleotide polymorphism haplotype diversity and recombination in the Isa gene of barley. Theor Appl Genet 2004, 109:543-551.
51. Russell J, Booth A, Fuller J, Harrower B, Hedley P, Machray G, Powell W. A comparison of sequence-based polymorphism and haplotype content in transcribed and anonymous regions of the barley genome. Genome 2004, 47:389-398. 10.1139/g03-125, 15060592.
52. Xu Y, Zhu L, Xiao J, Huang N, McCouch SR. Chromosomal regions associated with segregation distortion of molecular markers in F2 backcross doubled-haploid and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genet 1997, 253:535-545. 10.1007/s004380050355, 9065686.
53. Falque M, De'cousset L, Dervins D, Jacob A-M, Joets J, Martinant J-P, Raffoux X, Ribière N, Ridel C, Samson D, Charcosset A, Murigneux A. Linkage mapping of 1454 new maize candidate gene loci. Genetics 2005, 170:1957-1966. 10.1534/genetics.104.040204, 1449757, 15937132.
54. Ramu P, Kassahun B, Senthilvel S, Ashok Kumar C, Jayashree B, Folkertsma RT, Ananda Reddy L, Kuruvinashetti MS, Haussmann BIG, Hash CT. Exploiting rice-sorghum synteny for targeted development of EST-SSRs to enrich the sorghum genetic linkage map. Theor Appl Genet 2009, 119:1193-1204. 10.1007/s00122-009-1120-4, 19669123.
55. Talame' V, Ozturk NZ, Bohnert HJ, Tuberosa R. Barley transcript profiles under dehydration shock and drought stress treatments: a comparative analysis. J Exp Bot 2007, 58:229-240.
56. Sun J, Jiang H, Xu Y, Li H, Wu X, Xie Q, Li C. The CCCH type zinc finger proteins AtSZF1 and AtSZF2 regulate salt stress responses in Arabidopsis. Plant Cell Physiol 2007, 48:1148-1158. 10.1093/pcp/pcm088, 17609218.
57. Arora R, Agarwal P, Ray S, Singh AK, Singh VP, Tyagi AK, Kapoor S. MADS-box gene family in rice: genome-wide identification organization and expression profiling during reproductive development and stress. BMC Genomics 2007, 8:242. 10.1186/1471-2164-8-242, 1947970, 17640358.
58. Kottapalli KR, Rakwal R, Shibato J, Burow G, Tissue D, Burke J, Puppala N, Burow M, Payton P. Physiology and proteomics of the water-deficit stress response in three contrasting peanut genotypes. Plant Cell Environ 2009, 32:380-407. 10.1111/j.1365-3040.2009.01933.x, 19143990.
59. Cruz de Carvalho MH. Drought and reactive oxygen species: production scavenging and signalling. Plant Signal Behav 2008, 3:156-165. 10.4161/psb.3.3.5536, 2634109, 19513210.
60. Mao X, Zhang H, Tian S, Chang X, Jing R. TaSnRK24 an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.) confers enhanced multistress tolerance in Arabidopsis. J Exp Bot 2010, 61:683-696. 10.1093/jxb/erp331, 2814103, 20022921.
61. Mukhopadhyay A, Vij S, Tyagi AK. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration and salt stress in transgenic tobacco. Proc Natl Acad Sci USA 2004, 101:6309-6314. 10.1073/pnas.0401572101, 395965, 15079051.
62. Lenka SK, Katiyar A, Chinnuswamy V, Bansal KC. Comparative analysis of drought-responsive transcriptome in Indica rice genotypes with contrasting drought tolerance. Plant Biotech J 2010, 9:315-327.
63. Bhatnagar-Mathur P, Vadez V, Sharma K. Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Rep 2008, 27:411-424. 10.1007/s00299-007-0474-9, 18026957.
64. Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold and high-salinity stress conditions. Plant Physiol 2004, 136:2734-2746. 10.1104/pp.104.046599, 523337, 15333755.
65. Huang XY, Chao DY, Gao JP, Zhu MZ, Shi M, Lin HX. A previously unknown zinc finger protein DST regulates drought and salt tolerance in rice via stomatal aperture control. Genes Develop 2009, 23:1709-1713. 10.1101/gad.1834509, 2720263, 19651983.
66. Kong Z, Li M, Yang W, Xu W, Xue Y. A novel nuclear-localized CCCH-type zinc finger protein OsDOS is involved in delaying leaf senescence in rice. Plant Physiol 2006, 141:1376-1388. 10.1104/pp.106.082941, 1533915, 16778011.
67. Guo YH, Yu YP, Wang D, Wu C-A, Yang G-D, Huang J-G, Zheng C-C. GhZFP1 a novel CCCH-type zinc finger protein from cotton enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5. New Phytol 2009, 183:62-75. 10.1111/j.1469-8137.2009.02838.x, 19402879.
68. Wang D, Guo YH, Wu CA, Yang GD, Li YY, Zheng CC. Genome-wide analysis of CCCH zinc finger family in Arabidopsis and rice. BMC Genom 2008, 9:44.
69. Nam J, de Pamphilis CW, Ma H, Nei M. Antiquity and evolution of the MADS-box gene family controlling flower development in plants. Mol Biol Evol 2003, 20:1435-1447. 10.1093/molbev/msg152, 12777513.
70. Cooper B, Clarke JD, Budworth P, Kreps J, Hutchinson D, Park S, Guimil S, Dunn M, Luginbuhl P, Ellero C, Goff SA, Glazebrook J. A network of rice genes associated with stress response and seed development. Proc Natl Acad Sci USA 2003, 100:4945-4950. 10.1073/pnas.0737574100, 153660, 12684538.
71. Hayano-Kanashiro C, Calderón-Vázquez C, Ibarra-Laclette E, Herrera-Estrella L, Simpson J. Analysis of gene expression and physiological responses in three Mexican maize landraces under drought stress and recovery irrigation. PLoS One 2009, 4:e7531. 10.1371/journal.pone.0007531, 2766256, 19888455.
72. Mohammadi M, Kav NNV, Deyholos MK. Transcriptional profiling of hexaploid wheat (Triticum aestivum L.) roots identifies novel dehydration responsive genes. Plant Cell Environ 2007, 30:630-645. 10.1111/j.1365-3040.2007.01645.x, 17407540.
73. Mariac C, Jehin L, Saïdou AA, Thuillet A-C, Couderc M, Sire P, Jugde H, Adam H, Bezancon G, Pham J-L, Vigouroux Y. Genetic basis of pearl millet adaptation along an environmental gradient investigated by a combination of genome scan and association mapping. Mol Ecol 2011, 20:80-91. 10.1111/j.1365-294X.2010.04893.x, 21050293.
74. Tardif G, Kane NA, Adam H, Labrie L, Major G, Gulick P, Sarhan F, Laliberté J-F. Interaction network of proteins associated with abiotic stress response and development in wheat. Plant Mol Biol 2007, 63:703-718. 10.1007/s11103-006-9119-6, 17211514.
75. Setter TL, Yan J, Warburton M, Ribaut J-M, Xu Y, Sawkins M, Buckler ES, Zhang Z, Gore MA. Genetic association mapping identifies single nucleotide polymorphisms in genes that affect abscissic acid levels in maize floral tissues during drought. J Exp Bot 2010, 62:701-716. 3003815, 21084430.
76. Mishra RN, Reddy PS, Nair S, Markandeya G, Reddy AR, Sopory SK, Reddy MK. Isolation and characterization of expressed sequence tags (ESTs) from subtracted cDNA libraries of Pennisetum glaucum seedlings. Plant Mol Biol 2007, 64:713-732. 10.1007/s11103-007-9193-4, 17558562.
77. Focke M, Gieringer E, Schwan S, Jänsch L, Binder S, Braun HP. Fatty acid biosynthesis in mitochondria of grasses: malonyl-coenzyme A is generated by a mitochondrial-localized acetyl-coenzyme A carboxylase. Plant Physiol 2003, 133:875-884. 10.1104/pp.103.027375, 219060, 12972648.
78. Zuther E, Huang S, Jelenska J, Eilenberg H, Arnold EM, Su X, Sirikhachornkit A, Podkowinski J, Zilberstein A, Haselkorn R, Gornicki P. Complex nested promoters control tissue-specific expression of acetyl-CoA carboxylase genes in wheat. Proc Natl Acad Sci USA 2004, 101:1403-1408. 10.1073/pnas.0307846100, 337065, 14734803.
79. Kholová J, Hash CT, Kočová M, Vadez V. Constitutive water conserving mechanisms are correlated with the terminal drought tolerance of pearl millet (Pennisetum americanum L.). J Exp Bot 2010, 61:369-377. 10.1093/jxb/erp314, 2803210, 19861657.
80. Sharma PC, Sehgal D, Singh D, Singh G, Yadav RS. A major terminal drought tolerance QTL is also associated with reduced salt uptake and enhanced growth under salt stress. Mol Breed 2011, 27:207-222.
81. Chen X, Salamini F, Gebhardt C. A potato molecular function map for carbohydrate metabolism and transport. Theor Appl Genet 2001, 102:284-295.
82. Yadav RS, Sehgal D, Vadez V. Using genetic mapping and genomics approaches in understanding and improving drought tolerance in pearl millet. J Exp Bot 2011, doi:101093/jxb/erq265.
83. Feltus FA, Singh HP, Lohithaswa HC, Schulze SR, Silva TD, Paterson AH. A comparative genomics strategy for targeted discovery of single-nucleotide polymorphisms and conserved-noncoding sequences in orphan crops. Plant Physiol 2006, 140:1183-1191. 10.1104/pp.105.074203, 1435799, 16607031.
84. Sehgal D, Rajpal VR, Raina SN, Sasanuma T, Sasakuma T. Assaying polymorphism at DNA sequence level for new and novel genetic diversity diagnostics of the safflower (Carthamus tinctorius L.). Genetica 2009, 135:457-470. 10.1007/s10709-008-9292-4, 18649115.
85. Stam P. Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 1993, 3:739-744.
86. Voorrips RE. MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 2002, 93:77-78. 10.1093/jhered/93.1.77, 12011185.