Environmental aridity is associated with cytotype segregation and polyploidy occurrence in Brachypodium distachyon (Poaceae)

New Phytologist

Volumn 193, Issue 3, 2012, Pages 797-805

  Manzaneda, Antonio J ^a,b   Rey, Pedro J ^a   Bastida, Jesús M ^a   Weiss Lehman, Christopher ^b   Raskin, Evan ^b   Mitchell Olds, Thomas ^b  

^a UNIVERSITY OF JAÉN   (Spain)

^b DUKE UNIVERSITY   (United States)

Author keywords

Brachypodium distachyon; Carbon isotope discrimination; Cytotype segregation; Environmental aridity; Flow cytometry; Natural variation; Ploidy; Water use efficiency (WUE)

Indexed keywords

CARBON; RAIN; WATER;

ADAPTATION; ARIDITY; CHROMOSOME; DROUGHT RESISTANCE; ENVIRONMENTAL FACTOR; ENVIRONMENTAL STRESS; FLOW CYTOMETRY; FLOWERING; GRASS; POLYPLOIDY; TOLERANCE; WATER USE EFFICIENCY;

ARTICLE; BRACHYPODIUM; CHROMOSOME SEGREGATION; CYTOLOGY; DROUGHT; ECOTYPE; ENVIRONMENT; FLOWER; GENETICS; GEOGRAPHY; PHYSIOLOGY; POLYPLOIDY; SPAIN; STATISTICAL MODEL; TIME;

BRACHYPODIUM; CARBON ISOTOPES; CHROMOSOME SEGREGATION; DROUGHTS; ECOTYPE; ENVIRONMENT; FLOWERS; GEOGRAPHY; LOGISTIC MODELS; POLYPLOIDY; RAIN; SPAIN; TIME FACTORS; WATER;

IBERIAN PENINSULA;

BRACHYPODIUM; BRACHYPODIUM DISTACHYON; CUCURBITA; POACEAE;

EID: 84855817430     PISSN: 0028646X     EISSN: 14698137     Source Type: Journal    
DOI: 10.1111/j.1469-8137.2011.03988.x     Document Type: Article

References (53)

1. Anthos. 2008. Information system of the plants of Spain. Real Jardín Botánico, CSIC - Fundación Biodiversidad. [WWW document]. URL: [accessed on 2 March 2008].
2. Baack EJ. 2004. Cytotype segregation on regional and microgeographic scales in snow buttercups (Ranunculus adoneus: Ranunculaceae). American Journal of Botany 91: 1783-1788.
3. Baack EJ. 2005. Ecological factors influencing tetraploid establishment in snow buttercups (Ranunculus adoneus, Ranunculaceae): minority cytotype exclusion and barriers to triploid formation. American Journal of Botany 92: 1827-1835.
4. Baack EJ, Stanton ML. 2005. Ecological factors influencing tetraploid speciation in snow buttercups (Ranunculus adoneus): niche differentiation and tetraploid establishment. Evolution 59: 1936-1944.
5. Balao F, Casimiro-Soriguer R, Talavera M, Herrera J, Talavera S. 2009. Distribution and diversity of cytotypes in Dianthus broteri as evidenced by genome size variations. Annals of Botany 104: 965-973.
6. Besnard G, García-Verdugo C, Rubio de Casas R. 2008. Polyploidy in the olive (Olea europaea): evidence from flow cytometry and nuclear microsatellite analyses. Annals of Botany 101: 25-30.
7. Brochmann C, Brysting AK, Alsos IG, Borgen L, Grundt HH, Scheen AC, Elven R. 2004. Polyploidy in arctic plants. Biological Journal of the Linnean Society 82: 521-536.
8. Buggs RJA, Pannell JR. 2006. Rapid displacement of a monoecious plant lineage is due to pollen swamping by a dioecious relative. Current Biology 16: 996-1000.
9. Buggs RJA, Pannell JR. 2007. Ecological differentiation and diploid superiority across a moving ploidy contact zone. Evolution 61: 125-140.
10. Catalán P, Olmstead RG. 2000. Phylogenetic reconstruction of the genus Brachypodium P. Beauv. (Poaceae) from combined sequences of chloroplast ndhF gene and nuclear ITS. Plant Systematics and Evolution 220: 1-19.
11. Doležel J, Bartos J. 2005. Plant DNA flow cytometry and estimation of nuclear genome size. Annals of Botany 95: 99-110.
12. Duchoslav M, Šafářová L, Krahulec F. 2010. Complex distribution patterns, ecology and coexistence of ploidy levels of Allium oleraceum (Alliaceae) in the Czech Republic. Annals of Botany 105: 719-735.
13. Farquhar GD, Ehleringer JR, Hubick KT. 1989. Carbon isotope discrimination and photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology 40: 503-537.
14. Firth D. 1993. Bias reduction of maximum likelihood estimates. Biometrika 80: 27-38.
15. Gibson DJ. 2009. Grasses and grassland ecology. New York, NY, USA: Oxford University Press.
16. Hasterok R, Draper J, Jenkins G. 2004. Laying the cytotaxonomic foundations of a new model grass, Brachypodium distachyon (L.) Beauv. Chromosome Research 12: 397-403.
17. Heschel MS, Donohue K, Hausmann N, Schmitt J. 2002. Population differences and natural selection for water-use efficiency in Impatiens capensis (Balsaminaceae). International Journal of Plant Science 163: 907-912.
18. Husband BC, Schemske DW. 1998. Cytotype distribution at a diploid-tetraploid contact zone in Chamerion (Epilobium) angustifolium (Onagracae). American Journal of Botany 85: 1688-1694.
19. Johnson MTJ, Husband BC, Burton TL. 2003. Habitat differentiation between diploid and tetraploid Galax urceolata (Diapensiaceae). International Journal of Plant Science 164: 703-710.
20. Keeler KH. 1990. Distribution of polyploid variation in big bluestem (Andropogon gerardii, Poaceae) across the tallgrass prairie region. Genome 33: 95-100.
21. Knight CA, Vogel H, Kroymann J, Shumate A, Witsenboer H, Mitchell-Olds T. 2006. Expression profiling and local adaptation of Boechera holboelli populations for water use efficiency across a naturally occurring water stress gradient. Molecular Ecology 15: 1229-1237.
22. Kubátová B, Travnicek P, Bastlova D, Curn V, Jarolimova V, Suda J. 2008. DNA ploidy-level variation in native and invasive populations of Lythrum salicaria at a large geographical scale. Journal of Biogeography 35: 167-176.
23. Levin DA. 2001. 50years of plant speciation. Taxon 50: 69-91.
24. Levin DA. 2002. The role of chromosomal change in plant evolution. New York, NY, USA: Oxford University Press.
25. Levy AA, Feldman M. 2002. The impact of polyploidy on grass genome evolution. Plant Physiology 130: 1587-1593.
26. Li WL, Berlyn GP, Ashton PMS. 1996. Polyploids and their structural and physiological characters relative to water deficit in Betula papyrifera (Betulaceae). American Journal of Botany 83: 15-20.
27. Liancourt P, Tielbörger K. 2009. Competition and a short growing season lead to ecotypic differentiation at the two extremes of the ecological range. Functional Ecology 23: 397-404.
28. Lumaret R, Guillerm J-L, Delay J, Ait Lhaj Loutfi A, Izco J, Jay M. 1987. Polyploidy and habitat differentiation in Dactylis glomerata L. from Galicia (Spain). Oecologia 73: 436-446.
29. Luo N, Liu J, Yub X, Jiang Y. 2011. Natural variation of drought response in Brachypodium distachyon. Physiologia Plantarum 141: 19-29.
30. Maherali H, Walden AE, Husband BC. 2009. Genome duplication and the evolution of physiological responses to water stress. New Phytologist 184: 721-731.
31. Manly BFJ. 1991. Randomization and Monte Carlo methods in biology. London, UK & New York, NY, USA: Chapman and Hall.
32. Martin SL, Husband BC. 2009. Influence of phylogeny and ploidy on species ranges of North American angiosperms. Journal of Ecology 97: 913-922.
33. McKay JK, Richards JH, Mitchell-Olds T. 2003. Genetics of drought adaptation in Arabidopsis thaliana: I. Pleitropy contributes to genetic correlations among ecological traits. Molecular Ecology 12: 1137-1151.
34. Mur LAJ, Allainguillaume J, Catalán P, Hasterok R, Jenkins G, Lesniewska K, Thomas I, Vogel JP. 2011. Exploiting the Brachypodium tool box in cereal and grass research. New Phytologist 191: 334-347.
35. Ninyerola M, Pons X, Roure JM. 2005. Atlas Climático Digital de la Península Ibérica. Metodología y aplicaciones en bioclimatología y geobotánica. ISBN 932860-8-7. Universidad Autónoma de Barcelona, Bellaterra, Spain. [WWW doument]. URL: [accessed on 5 April 2010].
36. Opanowicz M, Vain P, Draper J, Parker D, Doonan JH. 2008. Brachypodium distachyon: making hay with a wild grass. Trends in Plant Science 13: 172-177.
37. Otto SP. 2007. The evolutionary consequences of polyploidy. Cell 131: 452-462.
38. Parisod C, Holderegger R, Brochmann C. 2010. Evolutionary consequences of autopolyploidy. New Phytologist 186: 5-17.
39. Petit C, Bretagnolle F, Felber F. 1999. Evolutionary consequences of diploid-polyploid hybrid zones in wild species. Trends in Ecology and Evolution 14: 306-311.
40. Raabová J, Fischer M, Munzbergová Z. 2008. Niche differentiation between diploid and hexaploid Aster amellus. Oecologia 158: 463-472.
41. Ramsey J. 2011. Polyploidy and ecological adaptation in wild yarrow. Proceedings of the National Academy of Sciences, USA 108: 7096-7101.
42. Robertson IH. 1981. Chromosome numbers in Brachypodium Beauv. (Gramineae). Genetica 56: 55-60.
43. Savage JA, Cavender-Bares JM. 2011. Contrasting drought survival strategies of sympatric willows (genus: Salix): consequences for coexistence and habitat specialization. Tree Physiology 31: 604-614.
44. Schwartz CJ, Doyle MR, Manzaneda AJ, Rey PJ, Mitchell-Olds T, Amasino RM. 2010. Natural variation of flowering time and vernalization responsiveness in Brachypodium distachyon. Bioenergy Research 3: 38-46.
45. Shang Y, Ma L, Wang H, Feng W, Chen P, Cao X, Liu D, Wang X-E. 2011. The evolutionary history of PDR in Brachypodium distachyon polyploids. Molecular Biology Reports 38: 2211-2217.
46. Soltis DE, Buggs RJA, Doyle JJ, Soltis PS. 2010. What we still don't know about polyploidy. Taxon 59: 1387-1403.
47. Soltis DE, Soltis PS, Tate JA. 2004. Advances in the study of polyploidy since Plant Speciation. New Phytologist 161: 173-191.
48. Sonnleitner M, Flatscher R, Escobar García P, Rauchová J, Suda J, Schneeweiss GM, Hülber K, Schönswetter P. 2010. Distribution and habitat segregation on different spatial scales among diploid, tetraploid and hexaploid cytotypes of Senecio carniolicus (Asteraceae) in the Eastern Alps. Annals of Botany 106: 967-977.
49. Suda J, Kron P, Husband BC, Trávnícek P. 2007. Flow cytometry and ploidy: applications in plant systematic, ecology and evolutionary biology. In: Doležel J, Greilhuber J, Suda J, eds. Flow cytometry with plant cells. Weinheim, Germany: Wiley-VCH, 103-130.
50. Treier UA, Broennimann O, Normand S, Guisan A, Schaffner U, Steinger T, Müller-Schärer H. 2009. Shift in cytotype frequency and niche space in the invasive plant Centaurea maculosa. Ecology 90: 1366-1377.
51. Vogel JP, Garvin DF, Leong OM, Hayden DM. 2006. Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon. Plant Cell, Tissue and Organ Culture 84: 199-211.
52. Vogel JP, Tuna M, Budak H, Huo N, Gu YQ, Steinwand MA. 2009. Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon. BMC Plant Biology 9: 88.
53. Wolny E, Lesniewska K, Hasterok R, Langdon T. 2011. Compact genomes and complex evolution in the genus Brachypodium. Chromosoma 120: 199-212.