Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Patel, Asmita ^a   Muñoz, Anisleidys ^a,b   Halvorsen, Katherine ^a   Rai, Priyamvada ^a,c  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b Department of Biology   (United States)

^c UNIVERSITY OF MIAMI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CDNA LIBRARIES; CLONING VECTORS; COMPLEMENTARY SEQUENCES; DNA POLYMERASE; EXONUCLEASE ACTIVITY; GENE OVER-EXPRESSION; GENE TRANSDUCTION; HIGH-THROUGHPUT; HUMAN LUNG; HUMAN PROSTATE CANCER CELLS; LABORATORY SCALE; MAMMALIAN CELLS; MITOTIC CELLS; OVER-EXPRESSION; RESTRICTION ENZYMES; RETROVIRAL TRANSDUCTION; RETROVIRAL VECTORS; TRANSDUCTION EFFICIENCY;

BACTERIOPHAGES; CELL CULTURE; CONVOLUTION; DISEASES; DNA; GENE EXPRESSION; POLYMERASE CHAIN REACTION; VECTORS;

CLONING;

CATALASE; COMPLEMENTARY DNA; DNA POLYMERASE; PROTEIN BAX; PROTEIN P53; RESTRICTION ENDONUCLEASE; RETROVIRUS VECTOR; SPLEEN EXONUCLEASE;

ARTICLE; CANCER CELL CULTURE; DNA SEQUENCE; ENZYME ACTIVITY; GENE INSERTION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; LIGATION INDEPENDENT CLONING; LUNG CANCER; MALE; MAMMAL CELL; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION; PROSTATE CANCER; STABLE EXPRESSION; VIRAL GENE DELIVERY SYSTEM;

BCL-2-ASSOCIATED X PROTEIN; CATALASE; CELL LINE, TUMOR; CLONING, MOLECULAR; DNA RESTRICTION ENZYMES; DNA, COMPLEMENTARY; GENE EXPRESSION; GENETIC VECTORS; HUMANS; PLASMIDS; REPRODUCIBILITY OF RESULTS; RETROVIRIDAE; TRANSDUCTION, GENETIC; TUMOR SUPPRESSOR PROTEIN P53;

MAMMALIA;

EID: 84855789039     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-3     Document Type: Article

References (12)

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