Strain improvement in Trichoderma viride through mutation for overexpression of cellulase and characterization of mutants using random amplified polymorphic DNA (RAPD)

African Journal of Biotechnology

Volumn 10, Issue 84, 2011, Pages 19590-19597

  Shafique, Shazia ^a   Bajwa, Rukhsana ^a   Shafique, Sobiya ^a  

^a UNIVERSITY OF THE PUNJAB   (Pakistan)

Author keywords

Cellulase activity; EMS treatment; RAPD PCR; Trichoderma viride; UV irradiation

Indexed keywords

CELLULASE; MESYLIC ACID ETHYL ESTER;

AGAR DIFFUSION; ARTICLE; CONTROLLED STUDY; DNA FRAGMENTATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; FUNGAL GENOME; FUNGAL STRAIN; FUNGUS ISOLATION; FUNGUS MUTANT; FUNGUS MUTATION; GENETIC DIFFERENCE; GENETIC STABILITY; GENETIC VARIABILITY; GENOTYPE; MOLECULAR PHYLOGENY; MUTAGENESIS; NONHUMAN; PHYLOGENETIC TREE; RANDOM AMPLIFIED POLYMORPHIC DNA; STRAIN IMPROVEMENT; TRICHODERMA VIRIDE; ULTRAVIOLET RADIATION;

FUNGI; TRICHODERMA VIRIDE;

EID: 84855782145     PISSN: 16845315     EISSN: None     Source Type: Journal    
DOI: 10.5897/AJB10.2464     Document Type: Article

References (22)

1. Azin DA, Noroozi RF (2001). Effect of chemicals on the improved gluconate productivity by an Aspergillus niger strain. Appl. Biochem. Biotechnol. 61(3): 393-397.
2. Beauchemin KA, Colombatto D, Morgavi DP, Yang WZ (2003). Use of exogenous fibrolytic enzymes to improve animal feed utilization by ruminants. J. Ani. Sci. 81(2): 37-47.
3. Chakraborty BN, Chakraborty U, Saha A, Dey PL, Sunar K (2010). Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their PCR-RAPD Profiles. Glob. J. Biotechnol. Biochem. 5(1): 55-61.
4. Chand P, Aruna A, Maqsood AM, Rao LV (2005). Novel mutation method for increased cellulase production. J. App. Microbiol. 98(2): 318-323.
5. Devehand M, Gwynne DI (1991). Expression of heterologous proteins in Aspergillus. J. Biotechnol. 17: 3-10.
6. th Ed. John Wiley and Sons, Inc. 304-305.
7. Hamad A, Haq I, Qadeer MA, Javed I (2001). Screening of Bacillus licheniformis mutants for improved production of alpha-amylase. Pak. Jour. Bot. 33(special issue): 517-525.
8. Jones C, Kortenkamp A (2000). RAPD library fingerprinting of bacterial and human DNA: Applications in mutation detection. Teratogen. Carcinogen. Mutagene. 20(2): 49-63.
9. Keinath AP, Farnham MW, Zitter TA (1995). Morphological, pathological, genetic differentiation of Didymella bryoniae and Phoma species isolated from cucurbits. Phytopathol. 85: 364-369.
10. Kotchoni OS, Shonukan OO (2002). Regulatory mutations affecting the synthesis of cellulose in B. pumilus. World J. Microbiol. Biotechnol. 18: 487-491.
11. McDermott JM, Brandle U, Dutty F, Haemmerli UA, Keller S, Muller KE, Wolfe MS (1994). Genetic variation in powdery mildew of barly: Development of RAPD, Scar and VNTR markers. Phytopathol. 84: 1316-1321.
12. Miller GL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Ana. Chem. 31: 426-428.
13. Minitab (2004). Multivariate cluster analysis. Compact disc software version. 13.0, USA.
14. Mohsin MJ (2006). Improvement of a thermophilic fungal strain for cellulase production by chemical and UV mutagenesis, Ph. D. thesis, Dept. of Bot. GC Univ. Lahore. Ph. D. thesis.
15. Morikawa Y, Kawamori M, Ado I, Shinsha I, Oda F, Takasawasa S, (1985). Improvement of cellulase production in Trichoderma reesei. Agric. Bio. Chem. 49: 1869.
16. Nei N, Li W (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Aca. Sci. USA 76: 5269-5273.
17. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984). Fungal DNA Isolation. PNAS. 81: 8014-8018.
18. Schulein M (2002). Protein engineering of cellulases: a review. Biochem. Biophy. Acta 1543: 239-252.
19. Shafique S, Bajwa R, Shafique S (2009). Cellulase biosynthesis by selected Trichoderma species. Pak. J. Bot. 41(2): 907-916.
20. Steel RGD, Torrie JH (1980). Principles and procedures of statistics, McGraw Hill Book Co., Inc. New York USA.
21. Sun Y, Cheng J (2002). Hydrolysis of lignocellulosic material from ethanol production: a review. Biores. Tech. 83: 1-11.
22. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids Res. 18: 6531-6535.