Multiplex PCR for the detection of five important Staphylococcus sp. in bovine subclinical mastitis milk

Indian Journal of Animal Sciences

Volumn 82, Issue 1, 2012, Pages 9-14

  Shome, Bibek Ranjan ^a   Das Mitra, Susweta ^a   Bhuvana, Mani ^a   Krithiga, Natesan ^a   Shome, Rajeswari ^a   Velu, Dhanikachalam ^a   Prabhudas, Krishnamshetty ^a  

^a ICAR NATIONAL INSTITUTE OF VETERINARY EPIDEMIOLOGY AND DISEASE INFORMATICS   (India)

Author keywords

Bovine mastitis; Coagulase negative staphylococcus; Diagnosis; Multiplex PCR; Staphylococcus aureus

Indexed keywords

BACTERIA (MICROORGANISMS); BOVINAE; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS CHROMOGENES; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HAEMOLYTICUS; STAPHYLOCOCCUS SCIURI; STAPHYLOCOCCUS SP.;

EID: 84855535591     PISSN: 03678318     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Bansal B K and Gupta D K. 2009. Economic Analysis of bovine mastitis in India and Punjab-A Review. Indian Journal of Animal Sciences 62 (3): 337-45.
2. Capurro A, Artursson K, Waller K P, Bengtsson B, Unnerstad E H and Aspan A. 2009. Comparison of a commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequence-based genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis. Veterinary Microbiology 134: 327-33.
3. Cremonesi P, Castiglioni B, Malferrari G, Biunno I, Vimercati C, Moroni P, Morandi S and Luzzana M. 2006. Improved method for rapid DNA extraction of mastitis pathogens directly from milk. Journal of Dairy Science 89: 163-69. (Pubitemid 43105920)
4. Ghebremedhin B, Layer F, Konig W and Konig B. 2008. Genetic classification and distinguishing of Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences. Journal of Clinical Microbiology 46: 1019-25. (Pubitemid 351379996)
5. Koskinen M T, Holopainen J, PyÖrälä S, Bredbacka P, Pitkala A, Barkema H W, Bexiga R, Roberson J, Solverod L, Piccinini R, Kelton D, Lehmusto H, Niskala S and Salmikivi L. 2009. Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. Journal of Dairy Science 92: 952-59.
6. Kuang Y, Tani K, Synnott A J, Ohshima K, Higuchi H, Nagahata H and Tanji Y. 2009. Characterization of bacterial population of raw milk from bovine mastitis by culture- independent PCRDGGE method. Journal of Biochemical Engineering 45: 76-81.
7. Kumar A P. 2009. Evaluation of PCR test for detecting major pathogens of bubaline mastitis directly from mastitic milk samples of buffaloes. Tropical Animal Health and Production 41: 1643-51.
8. Lee K H, Lee W J, Wang S W, Liu L Y, Lee F M, Chuang S T, Shy Y M, Chang C L and Chi C H. 2008. Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples. Journal of Veterinary Diagnostic Investigation 20: 463-71.
9. National Mastitis Council: Guidelines on normal and abnormal raw milk based on somatic cell counts and signs of clinical mastitis, National Mastitis Council, 2820 Walton Commons West, Suite 131, Madison, WI 53718, USA. www.nmconline.org.
10. Phuektes P. 2003. Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in bulk milk samples. Journal of Dairy Research 70: 149-55. (Pubitemid 36599403)
11. Pyörälä S and Taponen S, 2009. Coagulase-negative staphylococci emerging mastitis pathogens. Veterinary Microbiology 134: 3-8.
12. Ruegg L P. 2009. The quest for the perfect test: Phenotypic versus gnotypic identification of coagulase-negative staphylococci associated with bovine mastitis. Veterinary Microbiology 134: 15-19.
13. Sasidhar P V K, Reddy R Y, Rao and S G V. 2002. Economics of mastitis. Indian Journal of Animal Sciences 72 (4): 439-440. (Pubitemid 34969711)
14. Sindhu N, Sharma A and Jain V K. 2010. Molecular detection of Staphylococus aureus mastitis in crossbred cows based on genus specific gap gene and species specific aro A gene PCR assay. Indian Journal of Animal Sciences 80 (1): 3-6.
15. Singh J and Kataria A K. 2009. Relationships between bacterial species and total somatic cell counts in sub clinical and latent mastitis in cattle. Indian Journal of Animal Sciences 79 (1): 38-40.
16. Sharma A, Sindhu N and Jain V K. 2009. 16-23S rRNA intergenic spacer based molecular detection of Staphylococcus aureus directly from mastitic milk of crossbred cows. Indian Journal of Animal Sciences 79 (3): 350-52.
17. Taponen S, Salmikivi L, Simojoki H, Koskinen, M T and Pyörälä S. 2009. Real Time Polymerase chain reaction based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. Journal of Dairy Science 92: 2610-17.
18. Viguier C, Arora S, Gilmartin N, Welbeck K and O'Kennedy R. 2009. Mastitis detection: current trends and future perspectives. Trends in Biotechnology 27: 486-93.
19. Wilson D, Gonzalez R and Das H. 1997. Bovine mastitis pathogens in New York and Pennsylvania: Prevalence and effects on somatic cell count and milk production. Journal of Dairy Science 80: 2592-98.