Functional study of acetylcholinesterase genes in Bombyx mori ovary cells using RNA interference

Entomologia Experimentalis et Applicata

Volumn 142, Issue 2, 2012, Pages 140-144

  Cao, Y Q ^a   Liu, L H ^a,b   Wang, J M ^a   Wang, Y H ^a   Shen, W D ^a   Li, B ^a  

^a SOOCHOW UNIVERSITY   (China)

^b TONGHUA NORMAL UNIVERSITY   (China)

Author keywords

Ace genes; AChE; Bombycidae; Lepidoptera; Nucleopolyhedrovirus; QRT PCR; RNAi; Transcription

Indexed keywords

ENZYME ACTIVITY; FUNCTIONAL ROLE; GENE EXPRESSION; MOTH; POLYMERASE CHAIN REACTION; REAL TIME; RNA;

BOMBYCIDAE; BOMBYX MORI; BOMBYX MORI NPV; LEPIDOPTERA; NUCLEOPOLYHEDROVIRUS;

EID: 84855522778     PISSN: 00138703     EISSN: 15707458     Source Type: Journal    
DOI: 10.1111/j.1570-7458.2011.01207.x     Document Type: Article

References (30)

1. Arunkumar KP, Metta M & Nagaraju J (2006) Molecular phylogeny of silkmoths reveals the origin of domesticated silkmoth Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA. Molecular Phylogenetics and Evolution 40: 419-427.
2. Baek JH, Kim JI, Lee DW, Chung BK, Miyata T & Lee SH (2005) Identification and characterization of ace1-type acetylcholinesterase likely associated with organophosphate resistance in Plutella xylostella. Pesticide Biochemistry and Physiology 81: 164-175.
3. Chen HJ, Zhen L, Hui XM, Li GQ, Li F et al. (2009) Ace2, rather than ace1, is the major acetylcholinesterase in the silkworm, Bombyx mori. Insect Science 16: 297-303.
4. Duysen EG, Stribley JA, Fry DL, Hinrichs SH & Lockridge O (2002) Rescue of the acetylcholinesterase knockout mouse by feeding a liquid diet: phenotype of the adult acetylcholinesterase deficient mouse. Developmental Brain Research 137: 43-54.
5. Ellman GL, Courtney KD, Andres VJ & Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 17: 88-95.
6. Fournier D & Mutero A (1994) Modification of acetylcholinesterase as a mechanism of resistance to insecticides. Comparative Biochemistry and Physiology 108: 19-31.
7. Fournier D, Bride JM, Karch F & Bergé JB (1988) Acetylcholinesterase from Drosophila melanogaster- identification of two subunits encoded by the same gene. FEBS Letters 238: 333-337.
8. Grace TDC (1967) Establishment of a line of cells from the silkworm, Bombyx mori. Nature 216: 613.
9. Hall JC & Kankel DR (1976) Genetics of acetylcholinesterase in Drosophila melanogaster. Genetics 83: 517-535.
10. Hall LM & Spierer P (1986) The Ace locus of Drosophila melanogaster: structural gene for acetylcholinesterase with an unusual 5- leader. EMBO Journal 5: 2949-2954.
11. Huchard E, Martinez M, Alout H, Douzery EJP, Lutfalla G et al. (2006) Acetylcholinesterase genes within the Diptera: takeover and loss in true flies. Proceedings of the Royal Society of London, Series B 273: 2595-2604.
12. Kasprzak H & Salpeter MM (1985) Recovery of acetylcholinesterase at intact neuromuscular junctions after in vivo inactivation with di-isopropylfluorophosphate. Neuroscience 5: 951-955.
13. Kumar M, Gupta GP & Rajam MV (2009) Silencing of acetylcholinesterase gene of Helicoverpa armigera by siRNA affects larval growth and its life cycle. Journal of Insect Physiology 55: 273-278.
14. Lang GJ, Zhang MY, Li BL, Yu LL, Lu XM & Zhang CX (2010) Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans. BMB Reports 43: 573-578.
15. Lee DW, Kim SS, Shin SW, Kim WT & Boo KS (2006) Molecular characterization of two acetylcholinesterase genes from the oriental tobacco budworm, Helicoverpa assulta (Guenee). Biochimica et Biophysica Acta 1760: 125-133.
16. Li B, Wang YH, Liu HT, Xu YX, Wei ZG et al. (2010) Genotyping of acetylcholinesterase in insects. Pesticide Biochemistry and Physiology 98: 19-25.
17. Li B, Wang YH, Wang JM & Shen WD (2011) Cloning and expression analysis of acetylcholinesterase Gene (Bm-ace1, Bm-ace2) from domesticated silkworm, Bombyx mori. Advances in Materials Research 175-176: 13-18.
18. -△△CT method. Methods 25: 402-408.
19. Maeda S (1994) Expression of foreign genes in insect cells using baculovirus vectors. Insect Cell Biotechnology (ed. by K Maramorosch & AH McIntosh), pp. 1-31. CRC Press, Boca Raton, FL, USA.
20. Miao YG, Zhang YS, Nakagaki K, Zhao TF, Zhao AC et al. (2006) Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system. Applied Microbiology and Biotechnology 71: 192-199.
21. Mizuno H, Tomita T, Kasai S, Komagata O, Imanishi S & Kono Y (2006) Identification and expression of two types of acetylcholinesterases in a cultured cell line of Aedes albopictus, compared to mosquito whole body extracts. Applied Entomology and Zoology 41: 445-453.
22. Motohashi T, Shimojima T, Fukagawa T, Maenaka K & Park EY (2005) Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system. Biochemical and Biophysical Research Communications 326: 564-569.
23. Schefe JH, Lehmann KE, Buschmann IR, Unger T & Funke-Kaiser H (2006) Quantitative real-time RT-PCR data analysis: current concepts and the novel "gene expression's CT difference" formula. Journal of Molecular Medicine 84: 901-910.
24. Seino A, Kazuma T, Tan AJ, Tanaka H, Kono Y et al. (2007) Analysis of two acetylcholinesterase genes in Bombyx mori. Pesticide Biochemistry and Physiology 88: 92-101.
25. Shang JY, Shao YM, Lang GJ, Yuan G, Tang ZH & Zhang CX (2007) Expression of two types of acetylcholinesterase gene from the silkworm, Bombyx mori, in insect cells. Insect Science 14: 443-449.
26. Shimizu K, Shiomi K, Kajiura Z & Nakagaki M (2004) Nephila clavata flagelliform silk protein expressed in insect cell. Journal of Sericultural Science of Japan 73: 23-29.
27. Shinpei K, Nobumitsu H, Yutaka S, Hiroaki A, Sumio S et al. (2009) The Bombyx ovary-derived cell line endogenously expresses PIWI/PIWI-interacting RNA complexes. RNA Society 15: 1258-1264.
28. Voss G & Matsumura F (1964) Resistance to organophosphorus compounds in the two-spotted spider mite: two different mechanisms of resistance. Nature 202: 319-320.
29. Weill M, Fort P, Berthomieu A, Dubois MP, Pasteur N & Raymond M (2002) A novel acetylcholinesterase gene in mosquitoes codes for the insecticide target and is nonhomologous to the ace gene in Drosophila. Proceedings of the Royal Society of London, Series B 269: 2007-2016.
30. Xia QY, Zhou ZY, Lu C, Cheng DJ, Dai FY et al. (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306: 1937-1940.