Methods for separate isolation of cell-free DNA and cellular DNA from urine-application of Methylation-Specific PCR on both DNA fractions

Open Biomarkers Journal

Volumn 4, Issue 1, 2011, Pages 15-17

  Beermann, Agnes ^a   Ghanjati, Foued ^a   Hermanns, Thomas ^b   Poyet, Cedric ^b   Pereira, Joana ^a   Fischer, Johannes ^a   Wernet, Peter ^a   Santourlidis, Simeon ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

Cell free DNA; Diagnosis; DNA methylation; Urine

Indexed keywords

CELL DNA; DNA; PROTEINASE K;

ARTICLE; DNA DETERMINATION; DNA ISOLATION; DNA METHYLATION; DNA SEQUENCE; HUMAN; KINETICS; METHODOLOGY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEDIMENTATION; URINALYSIS;

EID: 84855513248     PISSN: None     EISSN: 18753183     Source Type: Journal    
DOI: 10.2174/1875318301104010015     Document Type: Article

References (9)

1. van Oorschot RA, Ballantyne KN, Mitchell RJ. Forensic traceDNA: a review. Investig Genet 2010; 1(1): 14.
2. Ray DA, Walker JA, Batzer MA. Mobile element-based forensicgenomics. Mutat Res 2007; 616(1-2): 24-33.
3. Proctor I, Stoeber K, Williams GH. Biomarkers in bladder cancer. Histopathology 2010; 57(1): 1-13.
4. Madu CO, Lu Y. Novel diagnostic biomarkers for prostate cancer. J Cancer 2010; 1: 150-77.
5. van der Vaart M, Pretorius PJ. The origin of circulating free DNA. Clin Chem. 2007; 53(12): 2215.
6. van der Vaart M, Pretorius PJ. Circulating DNA. Its origin andfluctuation. Ann N Y Acad Sci 2008; 1137: 18-26.
7. Seifert HH, Schmiemann V, Mueller M, et al. In situ detection ofglobal DNA hypomethylation in exfoliative urine cytology of patientswith suspected bladder cancer. Exp Mol Pathol 2007; 82(3):292-7.
8. Schulz WA, Hatina J. Epigenetics of prostate cancer: beyond DNAmethylation. J Cell Mol Med 2006; 10(1): 100-25.
9. Schulz WA. DNA methylation in urological malignancies (review). Int J Oncol 1998; 13(1): 151-67.