NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1

Biomolecular NMR Assignments

Volumn 5, Issue 2, 2011, Pages 131-133

  Parnham, Stuart ^a   Gaines, William A ^b   Duggan, Brendan M ^a   Marcotte Jr , William R ^b   Hennig, Mirko ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b CLEMSON UNIVERSITY   (United States)

Author keywords

Major ampullate spidroin; N terminal domain; Nephila clavipes; Spider dragline silk

Indexed keywords

CARBON; FIBROIN; NITROGEN; SPIDROIN 1;

ANIMAL; ARTICLE; CHEMISTRY; NUCLEAR MAGNETIC RESONANCE; PROTEIN TERTIARY STRUCTURE; SPIDER;

ANIMALS; CARBON ISOTOPES; FIBROINS; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, TERTIARY; SPIDERS;

EID: 84855368233     PISSN: 18742718     EISSN: 1874270X     Source Type: Journal    
DOI: 10.1007/s12104-010-9284-z     Document Type: Article

References (10)

1. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6: 277-293
2. Gaines WAT, Marcotte WR Jr (2008) Identification and characterization of multiple spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes. Insect Mol Biol 17: 465-474
3. Hinman MB, Lewis RV (1992) Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a twoprotein fiber. J Biol Chem 267: 19320-19324
4. Lewis RV (2006) Spider silk: ancient ideas for new biomaterials. Chem Rev 106: 3762-3774
5. Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensionalNMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog Nucl Magn Reson Spectrosc 34: 93-158
6. Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS?: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44: 213-223
7. Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59: 687-696
8. Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4: 171-180
9. Xu M, Lewis RV (1990) Structure of a protein superfiber: spider dragline silk. Proc Natl Acad Sci USA 87: 7120-7124
10. Yamazaki T, Forman-Kay JD, Kay LE (1993) Two-dimensional NMR experiments for correlating 13Cb and 1Hd/e chemical shifts of aromatic residues in 13C-labeled proteins via scalar couplings. J Am Chem Soc 115: 11054-11055