Bioengineering of coagulation factor VIII for efficient expression through elimination of a dispensable disulfide loop

Journal of Thrombosis and Haemostasis

Volumn 10, Issue 1, 2012, Pages 107-115

  Selvaraj, S R ^a   Scheller, A N ^a   Miao, H Z ^a   Kaufman, R J ^b   Pipe, S W ^a  

^a UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^b BURNHAM INSTITUTE   (United States)

Author keywords

Bioengineering; Disulfide bonds; Factor VIII; Hemophilia A; Secretion

Indexed keywords

CYSTEINE; GLYCINE; RECOMBINANT BLOOD CLOTTING FACTOR 8; SERINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOENGINEERING; CELL STRAIN COS1; CHO CELL; CONTROLLED STUDY; DISULFIDE BOND; GENETIC TRANSFECTION; HEMOPHILIA A; MOUSE; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN SECRETION; PROTEIN STABILITY; WILD TYPE;

CELL LINE; DISULFIDES; FACTOR VIII; HUMANS; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; TRANSFECTION;

EID: 84855363773     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/j.1538-7836.2011.04545.x     Document Type: Article

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