Development and validation of a cell-based SEAP reporter assay for the detection of neutralizing antibodies against an anti-IL-13 therapeutic antibody

Journal of Immunological Methods

Volumn 375, Issue 1-2, 2012, Pages 258-263

  Liao, Karen ^a   Sikkema, Dan ^a   Wang, Catherine ^a   Chen, Keguan ^a   DeWall, Stephen ^a   Lee, Thomas N ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

Cell based assay; IL 13; Inhibitory concentration; Neutralizing antibody; SEAP reporter

Indexed keywords

ALKALINE PHOSPHATASE; ALPHA INTERFERON; INTERLEUKIN 13; INTERLEUKIN 13 ANTIBODY; INTERLEUKIN 4; NEUTRALIZING ANTIBODY;

ANTIBODY DETECTION; ARTICLE; ASTHMA; CELL ASSAY; CELL STRAIN HEK293; CONTROLLED STUDY; CROSS REACTION; DOSE RESPONSE; DRUG CROSS REACTIVITY; FALSE POSITIVE RESULT; HUMAN; HUMAN CELL; IC 50; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

ALKALINE PHOSPHATASE; ANTIBODIES, ANTI-IDIOTYPIC; ANTIBODIES, MONOCLONAL; ANTIBODIES, NEUTRALIZING; ASTHMA; CELLS, CULTURED; CROSS REACTIONS; DOSE-RESPONSE RELATIONSHIP, DRUG; GENES, REPORTER; HEK293 CELLS; HUMANS; IMMUNOASSAY; INTERFERON-ALPHA; INTERLEUKIN-13; INTERLEUKIN-4; SENSITIVITY AND SPECIFICITY;

ORYCTOLAGUS CUNICULUS;

EID: 84855344982     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2011.09.015     Document Type: Article

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