Di(2-ethylhexyl)phthalate metabolites as markers for blood transfusion in doping control: Intra-individual variability of urinary concentrations

Drug Testing and Analysis

Volumn 3, Issue 11-12, 2011, Pages 892-895

  Solymos, E ^a   Guddat, S ^b   Geyer, H ^b   Thomas, A ^b   Thevis, M ^b   Schanzer W ^b  

^a EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^b GERMAN SPORT UNIVERSITY COLOGNE   (Germany)

Author keywords

Blood transfusion; DEHP metabolites; Liquid chromatography mass spectrometry; Sports drug testing

Indexed keywords

BIOMOLECULES; BLOOD; DIRECT INJECTION; DRUG PRODUCTS; ENZYMATIC HYDROLYSIS; ESTERS; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLITES;

BLOOD TRANSFUSION; DI(2-ETHYLHEXYL) PHTHALATE METABOLITE; DI(2-ETHYLHEXYL)PHTHALATE; DOPING CONTROL; DRUG TESTING; INDIVIDUAL VARIABILITY; LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIES; PHTHALATE METABOLITES; PHTHALATES; SPORT DRUG TESTING;

SPORTS;

BIOLOGICAL MARKER; GLUCURONIDE; ISOTOPE; MONO(2 ETHYL 5 HYDROXYHEXYL)PHTHALATE; MONO(2 ETHYL 5 OXOHEXYL)PHTHALATE; PHTHALIC ACID 2 ETHYLHEXYL MONOESTER; PHTHALIC ACID BIS(2 ETHYLHEXYL) ESTER; PHTHALIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BLOOD TRANSFUSION; CONTROLLED STUDY; DIET; DOPING; DRUG DETERMINATION; DRUG URINE LEVEL; ENVIRONMENTAL EXPOSURE; FEMALE; HUMAN; HUMAN EXPERIMENT; HYDROLYSIS; INJECTION; INTRARATER RELIABILITY; ISOTOPE DILUTION ASSAY; LIQUID CHROMATOGRAPHY; MALE; METABOLITE; NORMAL HUMAN; OCCUPATIONAL EXPOSURE; PRIORITY JOURNAL; SPORT; TANDEM MASS SPECTROMETRY; URINALYSIS;

EID: 84855214505     PISSN: 19427603     EISSN: 19427611     Source Type: Journal    
DOI: 10.1002/dta.377     Document Type: Article

References (16)

1. ATSDR. Toxicological Profile for Di (2-ethylhexyl) phthalate. US Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry, Atlanta, GA, 2002.
2. P. W. Albro, R. Thomas, L. Fishbein (1973) Metabolism of diethylhexyl phthalate by rats, isolation and characterisation of the urinary metabolite. J. Chromatogr. 1973, 76, 321.
3. H. M. Koch, H. M. Bolt, J. Angerer Di(2-ethylhexyl)phthalate (DEHP) metabolites in human urine and serum after a single oral dose of deuterium-labelled DEHP. Arch. Toxicol. 2004, 78, 123.
4. M. J. Silva, D. B. Barr, J. A. Reidy, M. Kato, N. A. Malek, C. C. Hodge, D. Hurtz, A. M. Calafat, L. L. Needham, J. W. Brock Glucuronidation patterns of common urinary and serum monoester phthalate metabolites. Arch. Toxicol. 2003, 77, 561.
5. N. Monfort, R. Ventura, A. Latorre, V. Belalcazar, M. López, J. Segura Urinary di-(2-ethylhexyl)phthalate metabolites in athletes as screening measure for illicit blood doping: a comparison study with patients receiving blood transfusion. Transfusion 2010, 50, 145.
6. E. Solymos, S. Guddat, H. Geyer, U. Flenker, A. Thomas, J. Segura, R. Ventura, P. Platen, M. Schulte-Mattler, M. Thevis, W. Schänzer Rapid determination of urinary di(2-ethylhexyl) phthalate metabolites based on liquid chromatography/tandem mass spectrometry as a marker for blood transfusion in sports drug testing. Anal. Bioanal. Chem. 2011, 401, 517.
7. WADA website. Available at: [November 2009].
8. CDC. Fourth National Report on Human Exposure to Environmental Chemicals. U.S. Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA, 2009.
9. CDC. Fourth National Report on Human Exposure to Environmental Chemicals - Updated Tables. U.S. Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA, 2010.
10. J. D. Peck, A. M. Sweeney, E. Symanski, J. Gardiner, M. J. Silva, A. M. Calafat, S. L. Schantz Intra- and inter-individual variability of urinary phthalate metabolite concentrations in hmong women of reproductive age. J. Expo. Sci. Environ. Epidemiol. 2010, 20, 90.
11. M. S. Park, Y. J. Yang, Y. P. Hong, S. Y. Kim, Y. P. Lee Assessment of Di (2-ethylhexyl) phthalate exposure by urinary metabolites as a function of sampling time. J. Prev. Med. Public Health 2010, 1043, 301.
12. J. J. Adibi, R. M. Whyatt, P. L. Williams, A. M. Calafat, D. Camann, R. Herrick, H. Nelson, H. K. Bhat, F. P. Perera, M. J. Silva, R. Hause Characterization of phthalate exposure among pregnant women assessed by repeat air and urine samples. Environ. Health Perspect. 2008, 116, 467.
13. H. Fromme, G. Bolte, H. M. Koch, J. Angerer, S. Boehmer, H. Drexler, R. Mayer, B. Liebl Occurrence and daily variation of phthalate metabolites in the urine of an adult population. Int. J. Hyg. Envir. Heal. 2007, 210, 1.
14. H. M. Koch, B. Rossbach, H. Drexler, J. Angerer Internal exposure of the general population to DEHP and other phthalates - determination of secondary and primary phthalate monoester metabolites in urine. Environ. Res. 2003, 93, 177.
15. J. L. Preau Jr, L.-Y. Wong, M. J. Silva, L. L. Needham, A. M. Calafat Variability over one week in the urinary concentrations of metabolites of diethyl phthalate and Di(2-Ethylhexyl) phthalate among 8 adults: an observational study. Environ. Health Perspect. 2010, 118, 1748.
16. J. A. Hoppin, J. W. Brock, B. J. Davis, D. D. Baird Reproducibility of urinary phthalate metabolites in first morning urine samples. Environ. Health Perspect. 2002, 110, 515.