Isolation of single, intact chromosomes from single, selected ovarian cancer cells for in situ hybridization and sequencing

Gene

Volumn 493, Issue 1, 2012, Pages 132-139

  Malecki, Marek ^a,c   Szybalski, Waclaw ^b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b Phoenix Biomolecular Engineering Foundation   (United States)

^c WUHS   (United States)

Author keywords

Chromosome 7; Epidermal growth factor receptor gene mutation deletion variant III (EGFRvIII); Fluorescent in situ hybridization; Gene therapy; Next generation sequencing; Ovarian cancer

Indexed keywords

DOUBLE STRANDED DNA; EPIDERMAL GROWTH FACTOR RECEPTOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ARTICLE; BLOOD; CANCER CELL; CANCER PATIENT; CELL ISOLATION; CHROMOSOME ISOLATION; FLUORESCENCE IN SITU HYBRIDIZATION; GENE DELETION; GENE MUTATION; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH; NEXT GENERATION SEQUENCING; NUCLEOTIDE SEQUENCE; OVARY CANCER; PERITONEAL FLUID; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TUMOR GENE;

CHROMOSOMES; DNA, RECOMBINANT; FEMALE; GENE TARGETING; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; OVARIAN NEOPLASMS; RECEPTOR, EPIDERMAL GROWTH FACTOR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES;

EID: 84655167605     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2011.11.044     Document Type: Article

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