The use of temperature-sensitive plasmids in mycobacteria

Methods in Molecular Biology

Volumn 465, Issue , 2008, Pages 229-242

  Portevin, Damien ^a   Malaga, Wladimir ^b   Guilhot, Christophe ^c  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

^b UNIVERSITÉ PAUL SABATIER   (France)

^c INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

Author keywords

Allelic exchange; Conditional mutants; Essentiality testing; Mycobacteria; Thermosensitive plasmids

Indexed keywords

CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS;

EID: 84555196915     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-207-6_15     Document Type: Article

References (19)

1. McFadden, J. (1996) Recombination in mycobacteria. Mol. Microbiol. 21, 205-211. (Pubitemid 26291527)
2. Parish, T., and Stoker, N.G. (1998) Electroporation of mycobacteria, in Mycobacteria protocols (Parish, T. and Stoker, N.G., eds.) Humana Press, Inc., Totowa, NJ, pp. 129-144.
3. Guilhot, C., Gicquel, B. and Martin, C. (1992) Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000. FEMS Microbiol. Lett. 98, 181-186.
4. Pelicic, V., Jackson, M., Reyrat, J.M., Jacobs Jr, W.R., Gicquel, B. and Guilhot; C. (1997) Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. U.S.A. 94, 10955-10960. (Pubitemid 27430842)
5. Jackson, M., Crick, D.C. and Brennan, P.J. (2000) Phosphatidylinositol is an essential phospholipid of mycobacteria. J. Biol. Chem. 275, 30092-30099.
6. Irani, V.R., Lee, S.H., Eckstein, T.M., Inamine, J.M., Belisle, J.T. and Maslow, J.N. (2004) Utilization of a ts-sacB selection system for the generation of a Mycobacterium avium serovar-8 specific glycopeptidolipid allelic exchange mutant. Ann. Clin. Microbiol. Antimicrob. 30, 3-18.
7. Reed, R.R. (1981) Transposon-mediated site-specific recombination: a defined in vitro system. Cell 25, 713-719. (Pubitemid 11022972)
8. Malaga, W., Perez, E. and Guilhot, C. (2003) Production of unmarked mutations in mycobacteria using site-specific recombination. FEMS Microbiol. Lett. 219, 261-268. (Pubitemid 36302607)
9. Portevin, D., de Sousa-D'Auria, C., Houssin, C., Grimaldi, C., Chami, M., Daffé, M. and Guilhot, C. (2004) A polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms. Proc. Natl. Acad. Sci. U.S.A. 101, 314-319. (Pubitemid 38084247)
10. Portevin, D., de Sousa-D'Auria, C., Montrozier, H., Houssin, C., Stella, A., Lanéelle, M.A., Bardou, F., Guilhot, C., and Daffé, M. (2005) The acyl-AMP ligase FadD32 and AccD4-containing acyl-CoA carboxylase are required for the synthesis of mycolic acids and essential for mycobacterial growth: identification of the carboxylation product and determination of the acyl-CoA carboxylase components. J. Biol. Chem. 280, 8862-8874. (Pubitemid 40409576)
11. Chalut, C., Botella, L., de Sousa-D'Auria, C. Houssin, C. and Guilhot, C. (2006) The nonredundant roles of two 4'-phosphopantetheinyl transferases in vital processes of Mycobacteria. Proc. Natl. Acad. Sci. U.S.A. 103, 8511-8516. (Pubitemid 43838486)
12. Snapper, S.B., Melton, R.E., Mustapha, S., Kieser, T. and Jacobs Jr, W.R. (1990) Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Molec. Microbiol. 4, 1911-1919.
13. Quandt, J., and Hynes, M.F. (1993) Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127, 15-21. (Pubitemid 23140440)
14. Zalacain, M., Gonzalez, A., Guerrero, M.C., Maltaliano, R.J., Malpartida, F. and Jimenez, A. (1986) Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus. Nucleic Acids Res. 14, 1565-1581. (Pubitemid 16145774)
15. Guilhot, C., Gicquel, B., Davies, J. and Martin, C. (1992) Isolation and analysis of IS6120, a new insertion sequence from Mycobacterium smegmatis. Mol. Microbiol. 6, 107-113.
16. Guilhot, C., Otal, I., van Rompaey, I., Martín, C. and Gicquel, B. (1994) Efficient transposition in mycobacteria: construction of Mycobacterium smegmatis insertional mutant libraries. J. Bacteriol. 176, 535-539. (Pubitemid 24034077)
17. Le Dantec, C., Winter, N., Gicquel, B., Vincent, V. and Picardeau, M. (2001) Genomic sequence and transcriptional analysis of a 23-kb mycobacterial linear plasmid: evidence for horizontal transfer and identification of plasmid maintenance systems. J. Bacteriol. 183, 2157-2164. (Pubitemid 32240382)
18. Sambrook, J., and Russell, D.W. (2001) Molecular Cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Pethe, K., Alonso, S., Biet, F., Delogu, G., Brennan, M.J., Locht, C. and Menozzi, F.D. (2001) The heparin-binding haemagglutinin of M. tuberculosis is required for extrapulmonary dissemination. Nature 412, 190-194. (Pubitemid 32674716)