Detection of NF-κB activity in skeletal muscle cells by electrophoretic mobility shift analysis

Methods in Molecular Biology

Volumn 798, Issue , 2012, Pages 505-516

  Dahlman, Jason M ^a   Guttridge, Denis C ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Atrophy; Differentiation; Muscular dystrophy; Myogenesis; NF B; Skeletal muscle; Transcription factors

Indexed keywords

IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PHOSPHORUS;

ANIMAL; ARTICLE; CELL CULTURE; CELL NUCLEUS; CHEMISTRY; GEL MOBILITY SHIFT ASSAY; ISOTOPE LABELING; METABOLISM; METHODOLOGY; MOUSE; OLIGONUCLEOTIDE PROBE; SKELETAL MUSCLE;

ANIMALS; CELL NUCLEUS; CELLS, CULTURED; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ISOTOPE LABELING; MICE; MUSCLE, SKELETAL; NF-KAPPA B; OLIGONUCLEOTIDE PROBES; PHOSPHORUS RADIOISOTOPES;

EID: 84555196412     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-343-1_30     Document Type: Article

References (10)

1. Fried M, Crothers DM (1981) Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res 9:6505-6525
2. Garner MM, Revzin A (1981) A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res 9:3047-3060
3. Revzin A, Ceglarek JA, Garner MM (1986) Comparison of ncleic acid-protein interactions in solution and in polyacrylamide gels. Anal Biochem 153:172-177
4. Chaturvedi MM, Kumar A, Darnay BG, Chainy GB, Agarwal S, Aggarwal BB (1997) Sanguinarine (pseudochelerythrine) is a potent inhibitor of NF-kappaB activation, IkappaBalpha phosphorylation, and degradation. J Biol Chem 272:30129-30134 (Pubitemid 27512211)
5. Cheshire JL, Baldwin AS, Jr (1997) Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation. Mol Cell Biol 17:6746-6754 (Pubitemid 27451222)
6. Dahlman JM, Bakkar N, He W, Guttridge DC (2010) NF-kappaB functions in stromal fibroblasts to regulate early postnatal muscle development. J Biol Chem 285:5479-5487
7. Dahlman JM, Wang J, Bakkar N, Guttridge DC (2009) The RelA/p65 subunit of NF-kappaB specifically regulates cyclin D1 protein stability: implications for cell cycle withdrawal and skeletal myogenesis. J Cell Biochem 106:42-51
8. Guttridge DC, Albanese C, Reuther JY, Pestell RG, Baldwin AS, Jr (1999) NF-kappaB controls cell growth and differentiation through transcriptional regulation of cyclin D1. Mol Cell Biol 19:5785-5799 (Pubitemid 29339933)
9. Kumar A, Lnu S, Malya R, Barron D, Moore J, Corry DB, Boriek AM (2003) Mechanical stretch activates nuclear factor-kappaB, activator protein-1, and mitogen-activated protein kinases in lung parenchyma: implications in asthma. Faseb J 17:1800-1811 (Pubitemid 37214774)
10. Mayo MW, Norris JL, Baldwin AS (2001) Ras regulation of NF-kappaB and apoptosis. Methods Enzymol 333:73-87