Silencing of gene expression by gymnotic delivery of antisense oligonucleotides

Methods in Molecular Biology

Volumn 815, Issue , 2012, Pages 333-346

  Soifer, Harris S ^a   Koch, Troels ^b   Lai, Johnathan ^b   Hansen, Bo ^b   Hoeg, Anja ^b   Oerum, Henrik ^b   Stein, C A ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b Santaris Pharma AS   (Denmark)

Author keywords

Antisense; gap mer; Gymnosis; Locked nucleic acid; Phosphorothioate; Silencing

Indexed keywords

ANTISENSE OLIGODEOXYNUCLEOTIDE; LOCKED NUCLEIC ACID; OLIGONUCLEOTIDE; PROTEIN;

ARTICLE; CULTURE TECHNIQUE; DOWN REGULATION; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; HUMAN; METHODOLOGY; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR CELL LINE; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; DOWN-REGULATION; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; HUMANS; OLIGODEOXYRIBONUCLEOTIDES, ANTISENSE; OLIGONUCLEOTIDES; PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION;

EID: 84555187851     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-424-7_25     Document Type: Article

References (9)

1. Lebedeva, I, Benimetskaya, L, Stein, CA, Vilenchik, M (2000) Delivery of oligonucleotides to cells. Euro J Pharm and Biopharm 50:101-119.
2. Akhtar, S, Basu, S, Wickstrom, E, Juliano, R (1991) Interactions of antisense DNA oligonucleotide analogs with phospholipid membranes (liposomes). Nucl Acids Res 19:5551-5559. (Pubitemid 21912954)
3. Stein, CA, Hansen, B, Lai, J, Wu, S, Voskresenskiy, A, Hoeg, A, Worm, J Hedtjarn, M, Souleimanian, N, Miller, P, Soifer, H, Castanotto, D, Benimetskaya, L, Oerum, H, Koch, T (2009) Effi cient gene silencing by delivery of locked nucleic acid antisense oligonucleotides unassisted by transfection reagents. Nucl Acids Res 38:e3.
4. Fisher, T, Terhorst, T, Cao, X, Wagner, R (1993) Intracellular disposition and metabolism of fl uorescently- labeled unmodifi ed and modifi ed oligonucleotides microinjected into mammalian cells. Nucl Acids Res 21:3857-3865. (Pubitemid 23254510)
5. Moulds, C, Lewis, J, Froehler, B, Grant,D, Huang, T, Milligan, J, Matteuci, M, Wagner, R (1995) Site and mechanism of antisense inhibition of C-5 propyne oligonucleotides. Biochem 34:5044-5053.
6. Gibbings, DJ, Ciaudo, C, Erhardt, M, and Voinnet, O (2009) Multivesicular bodies associate with components of miRNA effector complexes and modulate miRNA activity. Nat Cell Biol 11: 1143-1149.
7. Lee, YS, Pressman, S, Andress, AP, Kim, K, White, JL, Cassidy, JJ, Li, X, Lubell, K, Lim, do H, Cho, IS, Nakahara, K, Preall, JB, Bellare, P, Sontheimer, EJ, Carthew, RJ (2009) Silencing by small RNAs is linked to endosomal traffi cking. Nat Cell Biol 11: 1150-1160.
8. Straarup, E, Fisker, N, Hedtjarn, M, Lindholm, M, Rosenbohm, C, Aarup, V, Hansen, H, Oerum, H, Hansen, J, Koch, T (2010) Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B expression and improve the lipoprotein profi le of mice and non-human primates. Nucl Acids Res 38:7100-7111.
9. Koch, T, Rosenbohm, C, Hansen, HF, Hansen, B, Straarup, EM, Kauppinen, S (2008) Locked Nucleic Acid: Properties and therapeutic aspects. In: Kurreck, J. (ed) Therapeutic Oligonucleotides. RSC Publishing, Cambridge, p. 103-34.