Preparation of single-stranded DNA from PCR products with streptavidin magnetic beads

Nucleic Acid Therapeutics

Volumn 21, Issue 6, 2011, Pages 437-440

  Wilson, Robert ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

APTAMER; SINGLE STRANDED DNA; SODIUM HYDROXIDE; STREPTAVIDIN;

ALKALINITY; ARTICLE; BIOTINYLATION; DEVICES; DNA DENATURATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTAVIDIN MAGNETIC BEAD;

DNA, SINGLE-STRANDED; IMMUNOMAGNETIC SEPARATION; POLYMERASE CHAIN REACTION; SODIUM HYDROXIDE; STREPTAVIDIN; WATER;

EID: 84555178994     PISSN: 21593337     EISSN: None     Source Type: Journal    
DOI: 10.1089/nat.2011.0322     Document Type: Article

References (13)

1. AVCI-ADALI, M., PAUL, A., WILHELM, N., ZIEMER, G., and WENDEL, H.P. (2010). Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation. Molecules 15, 1-11.
2. BLANK, M., WEINSCHENK, T., PRIEMER, M., and SCHLUES-ENER, H. (2001). Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels-Selective targeting of endothelial regulatory protein pigpen. J. Biol. Chem. 276, 16464-16468.
3. DRABOVICH, A.P., BEREZOVSKI, M., OKHONIN, V., and KRYLOV, S.N. (2006). Selection of smart aptamers by methods of kinetic capillary electrophoresis. Anal. Chem. 78, 3171-3178. (Pubitemid 43726140)
4. GOLD, L., AYERS, D., BERTINO, J., BOCK, C., BOCK, A., BRODY, E.N., CARTER, J., DALBY, A.B., EATON, B.E., FITZWATER, T., FLATHER, D., FORBES, A., FOREMAN, T., FOWLER, C., GAWANDER, B., GOSS, M., GUNN, M., GUPTA, S., HALLADAY, D., HEIL, J., HEILIG, J., HICKE, B., HUSAR, G., JANJIC, N., JARVIS, T., JENNINGS, S., KATI-LIUS, E., KEENEY, T.R., KIM, N., KOCH, T.H., KRAEMER, S., KROISS, L., LE, N., LEVINE, D., LINDSEY, W., LOLLO, B., MAYFIELD, W., MEHAN, M., MEHLER, R., NELSON, S.K., NELSON, M., NIEUWLANDT, D., NIKRAD, M., OCHSNER, U., OSTROFF, R.M., OTIS, M., PARKER, T., PIETRASIEWICZ, S., RESNICOW, D.I., ROHLOFF, J., SANDERS, G., SATTIN, S., SCHNEIDER, D., SINGER, B., STANTON, M., STERKEL, A., STEWART, A., STRATFORD, S., VAUGHT, J.D., VRKlJAN, M., WALKER, J.J., WATROBKA, M., WAUGH, S., WEISS, A., WILCOX, S.K., WOLFSON, A., WOLK, S.K., ZHANG, C., and ZICHI, D. (2010). Aptamer-based multiplexed proteomic technology for biomarker discovery. PLoS One 5, e15004.
5. KWAME, S., SHANGGUAN, D., XIONG, X., O'DONOGHUE, M.B., and TAN, W.H. (2010). Development of DNA aptamers using cell-SELEX. Nat. Protocols 5, 1169-1185.
6. LOU, X.H., QIAN, J.R., XIAO, Y., VIEL, L., GERDON, A.E., LAG-ALLY, E.T., ATZBERGER, P., TARASOW, T.M., HEEGER, A.J., and SOH, H.T. (2009). Micromagnetic selection of aptamers in microfluidic channels. Proc. Nat. Acad. Sci. USA 106, 2989-2994.
7. MARICIC, T., and PAABO, S. (2009). Optimisation of 454 sequencing library preparation from small amounts of DNA permits sequence determination of both DNA strands. Bio-Techniques 46, 51-57.
8. MURPHY, M.B., FULLER, S.T., RICHARDSON, P.M., and DOYLE, S.A. (2003). An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Res. 31, e110.
9. OH, S.S., PLAKOS, K., LOU, X.H., XIAO, Y., and SOH, H.T. (2010). In vitro selection of structure-switching, self-reporting aptamers. Proc. Nat. Acad. Sci. USA 107, 14053-14058.
10. PAUL, A., AVCI-ADALI, M., ZIEMER, G., and WENDEL, H.P. (2009). Streptavidin-coated magnetic beads for DNA strand separation implicate a multitude of problems during cell-SELEX. Oligonucleotides 19, 243-254.
11. SHUM, K.T., CHAN, C., LEUNG, C.M., and TANNER, J.A. (2011). Identification of a DNA aptamer that inhibits scler-ostin's antagonistic effect on Wnt signaling. Biochem. J. 434, 493-501
12. TOK, J.B.H., and FISCHER, N.O. (2008). Single microbead SE-LEX for efficient ssDNA aptamer generation against botuli-num neurotoxin. Chem. Commun. Apr. 28, 1883-1885. (Pubitemid 351503452)
13. WOCHNER, A., CECH, B., MENGER, M., ERDMANN, V.A., and GLOKLER, J. (2007). Semi-automated selection of DNA aptamers using magnetic particle handling. BioTechniques 43, 344-353. (Pubitemid 47417275)