Detection of cytoplasmic and nuclear functions of mTOR by fractionation

Methods in Molecular Biology

Volumn 821, Issue , 2012, Pages 105-124

  Rosner, Margit ^a   Hengstschläger, Markus ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

CHAPS; Cytoplasm; Cytoplasmic nuclear translocation; Fractionation; mTORC1; mTORC2; Nucleus

Indexed keywords

CRTC2 PROTEIN, HUMAN; MTORC1 COMPLEX, HUMAN; MULTIPROTEIN COMPLEX; PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL; ARTICLE; CELL FRACTIONATION; CELL NUCLEUS; CYTOPLASM; CYTOSOL; FIBROBLAST; GENE VECTOR; HUMAN; METABOLISM; METHODOLOGY; PHOSPHORYLATION; SIGNAL TRANSDUCTION;

ANIMALS; CELL FRACTIONATION; CELL NUCLEUS; CYTOPLASM; CYTOSOL; FIBROBLASTS; GENETIC VECTORS; HUMANS; MULTIPROTEIN COMPLEXES; PHOSPHORYLATION; PROTEINS; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS;

MAMMALIA;

EID: 84555178353     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-430-8_8     Document Type: Article

References (26)

1. Laplante M, Sabatini DM (2009) mTOR signaling at a glance. J Cell Sci 122:3589-3594.
2. Foster KG, Fingar DC (2010) Mammalian target of rapamycin (mTOR): conducting the cellular signaling symphony. J Biol Chem 19:14071-14077.
3. Averous J, Proud CG (2006) When translation meets transformation: the mTOR story. Oncogene 25:6423-6435. (Pubitemid 44582286)
4. Proud CG (2007) Signalling to translation: how signal transduction pathways control the protein synthetic machinery. Biochem J 403: 217-234. (Pubitemid 46596577)
5. Yang TT, Yu RY, Agadir A et al (2008) Integration of protein kinases mTOR and extracellular signal-regulated kinase 5 in regulating nucleocytoplasmic localiztaion of NFATc4. Mol Cell Biol 28:3489-3501. (Pubitemid 351657110)
6. Cunningham JT, Rodgers JT, Arlow DH et al (2007) mTOR controls mitochondrial oxidative function through a YY1-PGC-1alpha transcriptional complex. Nature 450:736-740. (Pubitemid 350207689)
7. Tsang CK, Liu H, Zheng XF (2010) mTOR binds to the promoters of RNA polymerase Iand III-transcribed genes. Cell Cycle 9: 953-957.
8. Desai BN, Myers BR, Schreiber SL (2002) FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction. Proc Natl Acad Sci USA 99:4319-4324. (Pubitemid 34285983)
9. Schieke SM, Phillips D, McCoy JP Jr et al (2006) The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity. J Biol Chem 281:27643-27652. (Pubitemid 44401789)
10. Drenan RM, Liu X, Bertram PG et al (2004) FKBP12-rapamycin-associated protein or mammalian target of rapamycin (FRAP/ mTOR) localization in the endoplasmic reticulum and Golgi apparatus. J Biol Chem 279:772-778. (Pubitemid 38044883)
11. Liu X, Zheng XF (2007) Endoplasmic reticulum and Golgi localization sequences for mammalian target of rapamycin. Mol Biol Cell 18:1073-1082. (Pubitemid 46399510)
12. Flinn RJ, Yan Y, Goswami S et al (2010) The late endosome is essential for mTORC1 signaling. Mol Biol Cell 21:833-841.
13. Sancak Y, Bar-Peled L, Zoncu R et al (2010) Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell 14:290-303.
14. Partovian C, Ju R, Zhuang ZW et al (2008) Syndecan-4 regulates subcellular localization of mTOR Complex2 and Akt activation in a PKCalpha-dependent manner in endothelial cells. Mol Cell 32:140-149.
15. Kim JE, Chen J (2000) Cytoplasmic-nuclear shuttling of FKBP12-rapamycin-associated protein is involved in rapamycin-sensitive signaling and translation initiation. Proc Natl Acad Sci USA 97:14340-14345. (Pubitemid 32016577)
16. Park IH, Bachmann R, Shirazi H et al (2002) Regulation of ribosomal S6 kinase by mammalian target of rapamycin. J Biol Chem 277: 31423-31429. (Pubitemid 34968942)
17. Zhang X, Shu L, Hosoi H et al (2002) Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture. J Biol Chem 277:28127-28134. (Pubitemid 34966765)
18. Bernardi R, Guernah I, Jin D et al (2006) PML inhibits HIF-1 alpha translation and neoangiogenesis through repression of mTOR. Nature 442:779-785. (Pubitemid 44258904)
19. Panasyuk G, Nemazanyy I, Zhyvoloup A et al (2009) mTORbeta splicing isoform promotes cell proliferation and tumorigenesis. J Biol Chem 284:30807-30814.
20. Goh ET, Pardo OE, Michael N et al (2010) Involvement of heterogeneous ribonucleoprotein F in the regulation of cell proliferation via the mammalian target of rapamycin/S6 kinase 2 pathway. J Biol Chem 285:17065-17076.
21. Kantidakis T, Ramsbottom BA, Birch JL et al (2010) mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf-1. Proc Natl Acad Sci USA 107:11823-11828.
22. Shor B, Wu J, Shakey Q et al (2010) Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells. J Biol Chem 285: 15380-15392.
23. Kim DH, Sarbassov DD, Ali SM et al (2002) mTOR interacts with raptor to form a nutrientsensitive complex that signals to the cell growth machinery. Cell 110:163-175. (Pubitemid 34876545)
24. Rosner M, Hengstschläger M (2008) Cytoplasmic and nuclear distribution of the protein complexes mTORC1 and mTORC2: rapamycin triggers dephosphorylation and delocalization of the mTORC2 components rictor and sin1. Hum Mol Genet 17: 2934-2948.
25. Rosner M, Fuchs C, Siegel N et al (2009) Functional interaction of mammalian target of rapamycin complexes in regulating mammalian cell size and cell cycle. Hum Mol Genet 18:3298-3310.
26. Rosner M, Siegel N, Fuchs C et al (2010) Efficient siRNA-mediated prolonged gene silencing in human amniotic fluid stem cells. Nat Protoc 5:1081-1095.