A simple polymerase chain reaction screen for homologous targeting in embryonic stem cells

Analytical Biochemistry

Volumn 332, Issue 2, 2004, Pages 401-403

  McDermott, Jeffrey ^a   Zhao, Ying ^b   Sauer, Brian ^a  

^a STOWERS INSTITUTE FOR MEDICAL RESEARCH   (United States)

^b OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

STEM CELLS;

EMBRYONIC STEM CELLS;

POLYMERASE CHAIN REACTION;

DNA; DNA POLYMERASE;

ACCURACY; ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; ELECTROPORATION; EMBRYO; EMBRYO CELL; GENE LOCUS; GENE TARGETING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; SEQUENCE HOMOLOGY; STEM CELL;

EID: 8444231589     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.05.027     Document Type: Article

References (6)

1. te Riele H., Maandag E.R., Berns A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl. Acad. Sci. USA. 89:1992;5128-5132
2. Thomas K.R., Capecchi M.R. Site-directed mutagenesis by gene targetting in mouse embryo-derived stem cells. Cell. 51:1987;503-512
3. Köntgen F., Stewart C.L. Simple screening procedure to detect gene targeting events in embryonic stem cells. Methods Enzymol. 225:1993;878-890
4. Ramírez-Solis R., Davis A.C., Bradley A. Gene targeting in embryonic stem cells. Methods Enzymol. 225:1993;855-878
5. Nitschke L., Kopf M., Lamers M.C. Quick nested PCR screening of ES cell clones for gene targeting events. Biotechniques. 14:1993;914-916
6. Tybulewicz V.L., Crawford C.E., Jackson P.K., Bronson R.T., Mulligan R.C. Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene. Cell. 65:1991;1153-1163