Thermally denaturing high-performance liquid chromatography analysis of primase activity

Analytical Biochemistry

Volumn 332, Issue 2, 2004, Pages 330-336

  Koepsell, Scott ^a,b   Bastola, Dhundy ^a   Hinrichs, Steven H ^a,b   Griep, Mark A ^b,c  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^b USA   (United States)

^c UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

DnaG primase; Enzyme Inhibitors; Enzymology; Escherichia coli; HPLC; Nucleic acid synthesis Inhibitors; Oligonucleotides

Indexed keywords

DENATURATION; ENZYMES; ESCHERICHIA COLI; OLIGONUCLEOTIDES; RADIOGRAPHY; RATE CONSTANTS; RNA;

COPOLYMER MICROSPHERES; DNAG PRIMASE; ELECTROPHORETIC SEPARATIONS; ENZYME INHIBITORS; ENZYMOLOGY; HPLC; POLYSTYRENE DIVINYLBENZENES; SCINTILLATION COUNTING;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

DIVINYLBENZENE; DNA PRIMASE; POLYMER; POLYSTYRENE; PRIMER DNA; RECOMBINANT DNA; RECOMBINANT RNA;

ALKYLATION; ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; DNA DENATURATION; DRUG ACTIVITY; DRUG SYNTHESIS; ELUTION; ENERGY YIELD; ENZYME ACTIVITY; ENZYME ASSAY; GEL MOBILITY SHIFT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; INFORMATION PROCESSING; ISOTOPE LABELING; KINETICS; NONHUMAN; PHASE SEPARATION; POROSITY; PRIORITY JOURNAL; PROKARYOTE; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; THERMAL ANALYSIS; THERMODYNAMICS;

ESCHERICHIA COLI; PROKARYOTA;

EID: 8444222286     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.06.019     Document Type: Article

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