Production, harvest, and concentration of feline immunodeficiency virus-based lentiviral vector from cells grown in CF10 or CF2 devices

Cold Spring Harbor Protocols

Volumn 7, Issue 1, 2012, Pages 118-123

  Saenz, Dyana T ^a   Barraza, Román ^a   Loewen, Nils ^a   Teo, Wulin ^a   Poeschla, Eric M ^a  

^a Simons Center for Quantitative Biology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EDETIC ACID; LENTIVIRUS VECTOR; TRYPSIN;

ARTICLE; CELL TRANSFER; CULTURE MEDIUM; FELINE IMMUNODEFICIENCY VIRUS; GOOD MANUFACTURING PRACTICE; NONHUMAN; PLASMID; PRECIPITATION; ROOM TEMPERATURE; SUPERNATANT; TISSUE CULTURE; ULTRACENTRIFUGE; CELL LINE; CULTURE TECHNIQUE; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY;

CELL CULTURE TECHNIQUES; CELL LINE; GENETIC VECTORS; HUMANS; IMMUNODEFICIENCY VIRUS, FELINE;

EID: 84255168814     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot067546     Document Type: Article

References (3)

1. Saenz DT, Barraza R, Loewen N, Teo W, Poeschla EM. 2012a. Feline immunodeficiency virus-based lentiviral vectors. Cold Spring Harb Protoc doi: 10.1101/pdb.ip067579.
2. Saenz DT, Barraza R, Loewen N, Teo W, Poeschla EM. 2012b. Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks. Cold Spring Harb Protoc doi: 10.1101/pdb.prot067553.
3. Saenz DT, Barraza R, Loewen N, Teo W, Poeschla EM. 2012c. Titration of feline immunodeficiency virus-based lentiviral vector preparations. Cold Spring Harb Protoc doi: 10.1101/pdb.prot067561.