ICSH recommendations for the measurement of Haemoglobin A 2

International Journal of Laboratory Hematology

Volumn 34, Issue 1, 2012, Pages 1-13

  Stephens, A D ^a   Angastiniotis, M ^b   Baysal, E ^c   Chan, V ^d   Fucharoen, S ^e   Giordano, P C ^f   Hoyer, J D ^g   Mosca, A ^h   Wild, B ⁱ  

^a UNIVERSITY COLLEGE LONDON HOSPITALS   (United Kingdom)

^b Dubai Health Authority ^*

^c DUBAI HEALTH AUTHORITY   (United Arab Emirates)

^d UNIVERSITY OF HONG KONG   (Hong Kong)

^e MAHIDOL UNIVERSITY   (Thailand)

^f LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^g Mayo Clinic College of Medicine   (United States)

^h UNIVERSITY OF MILAN   (Italy)

ⁱ UK NEQAS (H)   (United Kingdom)

Author keywords

Capillary electrophoresis; Haemoglobinopathy; Hb A 2; HPLC; Recommended methods; Thalassaemia

Indexed keywords

HEMOGLOBIN A2; LUTEINIZING HORMONE;

ARTICLE; BETA THALASSEMIA; CAPILLARY ELECTROPHORESIS; COLUMN CHROMATOGRAPHY; ELECTROPHORESIS; HEMOGLOBIN BLOOD LEVEL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LABORATORY TEST; PRIORITY JOURNAL;

BETA-THALASSEMIA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, CAPILLARY; HEMOGLOBIN A2; HETEROZYGOTE; HUMANS; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TERMINOLOGY AS TOPIC;

EID: 84155184261     PISSN: 17515521     EISSN: 1751553X     Source Type: Journal    
DOI: 10.1111/j.1751-553X.2011.01368.x     Document Type: Article

References (51)

1. Baglioni C. (1962) Abnormal human haemoglobins. VII. Chemical studies on haemoglobin D. Biochimica et Biophysica Acta 59, 437-449.
2. 2 and related performance data [Online]. Available at: (accessed 1 February 2011).
3. Baysal E. (2001) Hemoglobinopathies in the United Arab Emirates. Hemoglobin 25, 247-253.
4. Bouva M.J., Harteveld C.L., Van Delft P. & Giordano P.C. (2006) Known and new delta globin gene mutations and their diagnostic significance. Haematologica 91, 129-132.
5. Bunn H.F., Haney D.N., Kamin S., Gabbay K.H. & Gallop P.M. (1976) The biosynthesis of human hemoglobin A1c. Slow glycosylation of hemoglobin in vivo. Journal of Clinical Investigation 57, 1652-1659.
6. 2 concentrations in untreated and Fe-treated iron deficiency anaemia. Biomedicine 23, 85-87.
7. 2 concentrations in beta-thalassemia trait and effect of iron deficiency anaemia. Biomedicine 25, 282-284.
8. Chernoff A.I., Minnich V. & Chongchareonsuk S. (1954) Hemoglobin E, a hereditary abnormality of human hemoglobin. Science 120, 605-606.
9. Galacteros F., Amaudric F., Prehu C., Feingold N., Doucet-Populaire F., Sobel A. & Rosa J. (1993) Acquired unbalanced hemoglobin chain synthesis during HIV infection. Comptes Rendus de l'Academie des Sciences. Serie III, Sciences de la Vie 316, 437-440.
10. Galanello R., Turco M.P., Barella S., Giagu N., Dessi C., Cornacchia G. & Cao A. (1990) Iron stores and iron deficiency anemia in children heterozygous for beta-thalassemia. Haematologica 75, 319-322.
11. 2 levels: implication for beta-thalassemia carrier screening. American Journal of Hematology 46, 79-81.
12. Gerald P.S. & Ingram V.M. 1961. Recommendations for the nomenclature of hemoglobins. The Journal of Biological Chemistry 236, 2155-2156.
13. 2 values in an Italian population with a high prevalence of beta-thalassemia. Haematologica 93, 1380-1384.
14. Giordano P.C., Bakker-Verwij M. & Harteveld C.L. (2009) Frequency of alpha-globin gene triplications and their interaction with beta-thalassemia mutations. Hemoglobin 33, 124-131.
15. Hempe J.M. & Craver R.D. 1999. Laboratory diagnosis of structural hemoglobinopathies and Thalssemias by capillary isoelectric focusing. In: Clinical Applications of Capillary Electrophoresis (ed. S.M. Palfreys ), 81-98. Humana Press, Totowa, New Jersey.
16. Higgs D.R., Clegg J.B., Weatherall D.J., Serjeant B.E. & Serjeant G.R. (1984) Interaction of the alpha alpha alpha globin gene haplotype and sickle haemoglobin. British Journal Haematology 57, 671-678.
17. Huang X., Coleman W.F. & Zare R.N. (1989) Analysis of factors causing peak broadening in capillary zone electrophoresis. Journal of Chromatography 480, 95-110.
18. Huisman T.H.J. 1986. The Hemoglobinopathies. Methods in Hematology. Churchill Livingstone, Edinburgh.
19. Huisman T.H.J. & Dozy A.M. (1962) Studies on the heterogeneity of hemoglobin. V. Binding of hemoglobin with oxidised glutathione. Journal of Clinical and Laboratory Medicine 60, 302-318.
20. Hunt J.A. & Ingram V.M. (1960) Abnormal human haemoglobins. IV. The chemical difference between normal human haemoglobin and haemoglobin C. Biochimica et Biophysica Acta 42, 409-421.
21. Hunt J.A. & Ingram V.M. (1961) Abnormal human haemoglobins. VI. The chemical difference between haemoglobins A and E. Biochimica et Biophysica Acta 49, 520-536.
22. 2 reference preparation. British Journal of Haematology 38, 573-578.
23. Itano H.A. (1951) A third abnormal hemoglobin associated with hereditary hemolytic anemia. Proceedings of the National Academy of Sciences of the United States of America 37, 775-784.
24. Itano H.A. & Neel J.V. (1950) A new inherited abnormality of human hemoglobin. Proceedings of the National Academy of Sciences of the United States of America 36, 613-617.
25. Kattamis C., Lagos P., Metaxotou-Mavromati A. & Matsaniotis N. (1972) Serum iron and unsaturated iron-binding capacity in the -thalassaemia trait: their relation to the levels of haemoglobins A, A 2, and F. Journal of Medical Genetics 9, 154-159.
26. Kunkel H.G. & Bearn A.G. (1957) Minor hemoglobin components of normal human blood. Federation Proceedings 16, 760-762.
27. Kunkel H.G. & Wallenius G. (1955) New hemoglobin in normal adult blood. Science, New Series 122, 288.
28. Kunkel H.G., Ceppellinini R., Müller-Eberhard U. & Wolf J. (1957) Observations on the minor basic hemoglobin component in the blood of normal individuals and patients with thalassaemia. Journal of Clinical Investigation 36, 1615-1625.
29. Lehmann H. (1957) The hemoglobinopathies (Report of the ISH Meeting in Boston). Blood 12, 90-92.
30. 2 levels in beta-thalassaemia trait with coincident iron deficiency. Indian Journal of Pathology and Microbiology 41, 309-313.
31. Mantikou E., Arkesteijn S.G., Beckhoven Van J.M., Kerkhoffs J.L., Harteveld C.L. & Giordano P.C. (2009) A brief review on newborn screening methods for hemoglobinopathies and preliminary results selecting beta thalassemia carriers at birth by quantitative estimation of the HbA fraction. Clinical Biochemistry 42, 1780-1785.
32. 2 testing in the diagnosis of thalassaemias and related haemoglobinopathies. Journal of Clinical Pathology 62, 13-17.
33. Neel J. (1953) Statement concerning a system of nomenclature for the varieties of human hemoglobin. Blood 8, 386-387.
34. 2β-thalassaemia in Europe - characterisation of eight mutations, including a novel Poly A mutation (-AA). 10th International Conference on Thalassaemia & Haemoglobinopathies: abstracts published in conference proceedings, Singapore: EPI 49, 99.
35. Paglietti E., Galanello R., Addis M. & Cao A. (1985) Genetic counseling and genetic heterogeneity in the thalassemias. Clinical Genetics 28, 1-7.
36. 2 measurement: data from an Italian pilot study with fresh whole blood samples and commercial HPLC systems. Clinical Chemistry and Laboratory Medicine 45, 88-92.
37. 2. Clinical Chemistry and Laboratory Medicine 48, 1611-1618.
38. Pauling L., Itano H.A., Singer S.J. & Wells I.C. (1949) Sickle cell anemia, a molecular disease. Science 109, 443.
39. Phylipsen M., Gallivan M.V., Arkesteeijn S.G., Harteveld C.L. & Giordano P.C. (2011) Occurrence of common and rare δ-globin gene defects in two multiethnic populations:thirteen new mutationsand the significance of δ-globin gene defects in β-thalassaemia diagnostics. International Journal of Laboratory Hematology 33, 85-91.
40. 2 values in an HIV-1-infected patient receiving antiretroviral drugs: a pitfall for thalassemia antenatal diagnosis. Hemoglobin 33, 158-161.
41. Routy J.P., Monte M., Beaulieu R., Toma E., St-Pierre L. & Dumont M. (1993) Increase of hemoglobin A2 in human immunodeficiency virus-1-infected patients treated with zidovudine. American Journal of Hematology 43, 86-90.
42. Schnek A.G. & Schroeder W.A. 1961. The relation between the minor components of whole normal human adult hemoglobin as isolated by chromatography and starch block electrophoresis. Journal of the American Chemical Society 83, 1472-1478.
43. 2 levels in beta-thalassemia. American Journal of the Medical Sciences 305, 312-313.
44. Stern K., Reiner M. & Silber R. 1945. On the electrophoretic pattern of red blood cell proteins. Journal of Biological Chemistry 161, 731.
45. Tietz N., Finley P.R. & Pruden E.L. (1990) Clinical Guide to Laboratory Tests, 2nd edn. W.B. Saunders Company, Philadelphia.
46. Van Delft P., Lenters E., Bakker-Verweij M., De Korte M., Baylan U., Harteveld C.L. & Giordano P.C. (2009) Evaluating five dedicated automatic devices for haemoglobinopathy diagnostics in multi-ethnic populations. International Journal of Laboratory Hematology 31, 484-495.
47. 2 and E. Journal of Laboratory and Clinical Medicine 71, 85-91.
48. Weatherall D.J. & Clegg J.B. (2001) The Thalassaemia Syndrome. Blackwell Scientific, Oxford.
49. Wild B.J., Green B.N. & Stephens A.D. (2004) The potential of electrospray ionisation mass spectrometry for the diagnosis of hemoglobin variants found in newborn screening. Blood Cells, Molecules and Diseases 33, 308-317.
50. Wild B.J., Green B.N., Cooper E.K., Lalloz M.R.A., Erten S., Stephens A.D. & Layton D.M. (2001) Rapid identification of haemoglobin variants by electrospray ionisation mass spectrometry. Blood Cells, Molecules and Diseases 27, 691-704.
51. World Health Organization Expert Committee on Biological Standardization 1994. Biological Standardization Forty Fourth Report. WHO Technical Report Series - 848. World Health Organization, Geneva.