A new system for fast and quantitative analysis of heterologous gene expression in plants

New Phytologist

Volumn 193, Issue 2, 2012, Pages 504-512

  Thevenin J ^a   Dubos, C ^a   Xu, W ^a   Le Gourrierec, J ^b   Kelemen, Z ^a   Charlot, F ^a   Nogue F ^a   Lepiniec, L ^a   Dubreucq, B ^a  

^a INSTITUT JEAN PIERRE BOURGIN   (France)

^b UNIVERSITÉ D'ANGERS   (France)

Author keywords

cis element; Cytometry; Flavonoids; Physcomitrella patens; Protoplasts; Transcription factor

Indexed keywords

ARABIDOPSIS PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MULTIPROTEIN COMPLEX;

DICOTYLEDON; DISSECTION; FLOW CYTOMETRY; GENE EXPRESSION; PROTEIN; QUANTITATIVE ANALYSIS;

ARABIDOPSIS; ARTICLE; BINDING SITE; BIOLOGICAL MODEL; BRYOPSIDA; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETIC PROCEDURES; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; GENETICS; METABOLISM; PLANT SEED; PROMOTER REGION; PROTOPLAST; SACCHAROMYCES CEREVISIAE;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; BINDING SITES; BRYOPSIDA; GENE EXPRESSION; GENE EXPRESSION REGULATION, PLANT; GENETIC TECHNIQUES; GREEN FLUORESCENT PROTEINS; MODELS, GENETIC; MULTIPROTEIN COMPLEXES; PROMOTER REGIONS, GENETIC; PROTOPLASTS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SEEDS; TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC;

ARABIDOPSIS; ARABIDOPSIS THALIANA; PHYSCOMITRELLA; PHYSCOMITRELLA PATENS;

EID: 84155172191     PISSN: 0028646X     EISSN: 14698137     Source Type: Journal    
DOI: 10.1111/j.1469-8137.2011.03936.x     Document Type: Article

References (32)

1. Ashton NW, Cove DJ. 1977. The isolation and preliminary characterization of auxotrophic mutants of the moss, Physcomitrella patens. Molecular and General Genetics 154: 87-95.
2. Ashton NW, Cove DJ, Featherstone DR. 1979. The isolation and physiological analysis of mutants of the moss Physcomitrella patens, which over-produce gametophores. Planta 144: 437-442.
3. Baudry A, Caboche M, Lepiniec L. 2006. TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell-specific accumulation of flavonoids in Arabidopsis thaliana. Plant Journal 46: 768-779.
4. Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L. 2004. TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. Plant Journal 39: 366-380.
5. Berger B, Stracke R, Yatusevich R, Weisshaar B, Flugge UI, Gigolashvili T. 2007. A simplified method for the analysis of transcription factor-promoter interactions that allows high-throughput data generation. Plant Journal 50: 911-916.
6. Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J. 1996. Engineered GFP as a vital reporter in plants. Current Biology 6: 325-330.
7. Curtis MD, Grossniklaus U. 2003. A gateway cloning vector set for high-throughput functional analysis of genes inplanta. Plant Physiology 133: 462-469.
8. Dangl JL, Hauffe KD, Lipphardt S, Hahlbrock K, Scheel D. 1987. Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor. EMBO Journal 6: 2551-2556.
9. Debeaujon I, Nesi N, Perez P, Devic M, Grandjean O, Caboche M, Lepiniec L. 2003. Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development. Plant Cell 15: 2514-2531.
10. Dubos C, Le Gourrierec J, Baudry A, Huep G, Lanet E, Debeaujon I, Routaboul JM, Alboresi A, Weisshaar B, Lepiniec L. 2008. MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana. Plant Journal 55: 940-953.
11. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. 2010. MYB transcription factors in Arabidopsis. Trends in Plant Science 15: 573-581.
12. Ducrest AL, Amacker M, Lingner J, Nabholz M. 2002. Detection of promoter activity by flow cytometric analysis of GFP reporter expression. Nucleic Acids Research 30: e65.
13. Fukuda H, Ito M, Sugiyama M, Komamine A. 1994. Mechanisms of the proliferation and differentiation of plant cells in cell culture systems. International Journal of Developmental Biology 38: 287-299.
14. Galbraith DW, Anderson MT, Herzenberg LA. 1999b. Flow cytometric analysis and FACS sorting of cells based on GFP accumulation. Methods in Cell Biology 58: 315-341.
15. Galbraith DW, Herzenberg LA, Anderson MT. 1999a. Flow cytometric analysis of transgene expression in higher plants: green fluorescent protein. Methods in Enzymology 302: 296-315.
16. Hagenbeek D, Rock CD. 2001. Quantitative analysis by flow cytometry of abscisic acid-inducible gene expression in transiently transformed rice protoplasts. Cytometry 45: 170-179.
17. Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B. 1998. Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology 36: 741-754.
18. Horstmann V, Huether CM, Jost W, Reski R, Decker EL. 2004. Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude. BMC Biotechnology 4: 13.
19. Kalbin G, Strid Å, Frohnmeyer H. 1999. Transcriptional activation of the parsley chalcone synthase promoter in heterologous pea and yeast systems. Plant Physiology and Biochemistry 37: 821-829.
20. Kircher S, Wellmer F, Nick P, Rügner A, Schäfer E, Harter K. 1999. Nuclear import of the parsley bZIP transcription factor CPRF2 is regulated by phytochrome photoreceptors. Journal of Cell Biology 144: 201-211.
21. Lepiniec L, Debeaujon I, Routaboul J-M, Baudry A, Pourcel L, Nesi N, Caboche M. 2006. Genetics and biochemistry of seed flavonoids. Annual Review of Plant Biology 57: 405-430.
22. Marion J, Bach L, Bellec Y, Meyer C, Gissot L, Faure JD. 2008. Systematic analysis of protein subcellular localization and interaction using high-throughput transient transformation of Arabidopsis seedlings. Plant Journal 56: 169-179.
23. Matsui K, Umemura Y, Ohme-Takagi M. 2008. AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis. Plant Journal 55: 954-967.
24. Nakagawa T, Kurose T, Hino T, Tanaka K, Kawamukai M, Niwa Y, Toyooka K, Matsuoka K, Jinbo T, Kimura T. 2007. Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation. Journal of Bioscience and Bioengineering 104: 34-41.
25. Prigge MJ, Bezanilla M. 2010. Evolutionary crossroads in developmental biology: Physcomitrella patens. Development 137: 3535-3543.
26. Proust H, Hoffmann B, Xie X, Yoneyama K, Schaefer DG, Nogue F, Rameau C. 2011. Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Physcomitrella patens. Development 138: 1531-1539.
27. Quatrano RS, McDaniel SF, Khandelwal A, Perroud PF, Cove DJ. 2007. Physcomitrella patens: mosses enter the genomic age. Current Opinion in Plant Biology 10: 182-189.
28. Schaefer D, Zryd JP, Knight CD, Cove DJ. 1991. Stable transformation of the moss Physcomitrella patens. Molecular and General Genetics 226: 418-424.
29. Schaefer DG, Zrÿd JP. 1997. Efficient gene targeting in the moss Physcomitrella patens. Plant Journal 11: 1195-1206.
30. Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW. 1995. Green-fluorescent protein as a new vital marker in plant cells. Plant Journal 8: 777-784.
31. Trouiller B, Schaefer DG, Charlot F, Nogué F. 2006. MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens. Nucleic Acids Research 34: 232-242.
32. Xie DY, Sharma SB, Paiva NL, Ferreira D, Dixon RA. 2003. Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis. Science 299: 396-399.