Phosphatidylinositol 3,4,5-trisphosphate activity probes for the labeling and proteomic characterization of protein binding partners

Biochemistry

Volumn 50, Issue 51, 2011, Pages 11143-11161

  Rowland, Meng M ^a   Bostic, Heidi E ^a   Gong, Denghuang ^a   Speers, Anna E ^b   Lucas, Nathan ^c   Cho, Wonhwa ^c   Cravatt, Benjamin F ^b   Best, Michael D ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVITY PROBES; BIFUNCTIONAL ACTIVITY; BINDING PROTEINS; BIOLOGICAL PROCESS; CANCER CELLS; CELL EXTRACTS; CLICK CHEMISTRY; COMPLEX SAMPLES; COVALENT LABELING; FLUORESCENT DYES; LABELED PROTEINS; MEMBRANE ASSOCIATION; OPTICAL DETECTION; PHOSPHATIDYLINOSITOL; PHOTOAFFINITY; POLYPHOSPHATES; PROTEIN BINDING PARTNERS; PROTEIN DENATURATION; PROTEIN LABELING; PURIFIED PROTEIN; SITE-SPECIFIC; SYNTHETIC STRATEGIES; TANDEM MASS SPECTROMETRY; TARGET PROTEINS;

ACETYLENE; AFFINITY CHROMATOGRAPHY; ASSOCIATION REACTIONS; BIOCHEMISTRY; GELS; LIPIDS; MASS SPECTROMETRY; PROBES;

PROTEINS;

BIOTIN; CELL EXTRACT; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE; PROTEIN KINASE B;

ADHESION; AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DERIVATIZATION; ENZYME ACTIVITY; FUNCTIONAL PROTEOMICS; HUMAN; HUMAN CELL; MOLECULAR PROBE; NUCLEOTIDE SEQUENCE; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN TARGETING; SYNTHESIS; TANDEM MASS SPECTROMETRY;

ALKYNES; CELL LINE, TUMOR; CLICK CHEMISTRY; FLUORESCENT DYES; HUMANS; LIGANDS; MELANOMA; MEMBRANE PROTEINS; MOLECULAR PROBES; NEOPLASM PROTEINS; OSMOLAR CONCENTRATION; PEPTIDE FRAGMENTS; PHOSPHATIDYLINOSITOL PHOSPHATES; PHOTOAFFINITY LABELS; PROTEIN BINDING; PROTEOMICS; PROTO-ONCOGENE PROTEINS C-AKT; SOLUBILITY; TANDEM MASS SPECTROMETRY;

EID: 84055193026     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201636s     Document Type: Article

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