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Volumn 634, Issue , 2010, Pages 3-14

Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension

(1)  Alcalde, Miguel a  

a NONE

Author keywords

Combinatorial saturation mutagenesis; Directed evolution; In vivo recombination; IVOE; Saccharomyces cerevisiae

Indexed keywords

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 84055185824     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-652-8_1     Document Type: Article
Times cited : (36)

References (15)
  • 2
    • 0036897839 scopus 로고    scopus 로고
    • Milestones in directed enzyme evolution
    • Tao H, Cornish VW (2002) Milestones in directed enzyme evolution. Curr Opin Chem Biol 6:858-864
    • (2002) Curr Opin Chem Biol , vol.6 , pp. 858-864
    • Tao, H.1    Cornish, V.W.2
  • 3
    • 23444450226 scopus 로고    scopus 로고
    • Semi-rational approaches to engineering enzyme activity: Combining the benefits of directed evolution and rational design
    • Chica RA, Doucet N, Pelletier JN (2005) Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. Curr Opin Biotechnol 16:378-384
    • (2005) Curr Opin Biotechnol , vol.16 , pp. 378-384
    • Chica, R.A.1    Doucet, N.2    Pelletier, J.N.3
  • 8
    • 0034667190 scopus 로고    scopus 로고
    • High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: Statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2
    • Abecassis V, Pompon D, Truan G (2000) High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Res 28:1-10
    • (2000) Nucleic Acids Res , vol.28 , pp. 1-10
    • Abecassis, V.1    Pompon, D.2    Truan, G.3
  • 11
    • 0024520745 scopus 로고
    • Site-directed mutagenesis by overlap extension using the polymerase chain reaction
    • Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59
    • (1989) Gene , vol.77 , pp. 51-59
    • Ho, S.N.1    Hunt, H.D.2    Horton, R.M.3    Pullen, J.K.4    Pease, L.R.5
  • 12
    • 84859241501 scopus 로고    scopus 로고
    • Arnold FH, Gergiou G (eds) Directed evolution library creation. Methods and protocols. Humana Press, Totowa, NJ
    • Bulter T, Alcalde M (2003) Preparing libraries in S. cerevisiae. In: Arnold FH, Gergiou G (eds) Directed evolution library creation. Methods and protocols. Humana Press, Totowa, NJ, pp 17-22
    • (2003) Preparing Libraries in S. Cerevisiae , pp. 17-22
    • Bulter, T.1    Alcalde, M.2
  • 15
    • 0141923574 scopus 로고    scopus 로고
    • Screening mutant libraries in
    • Arnold FH, Gergiou G (eds) Directed enzyme evolution. Screening and selection methods, Humana Press, Totowa, NJ
    • Bulter T, Sieber V, Alcalde M (2003) Screening mutant libraries in Saccharomyces cerevisiae. In: Arnold FH, Gergiou G (eds) Directed enzyme evolution. Screening and selection methods, vol 230. Humana Press, Totowa, NJ, pp 99-108
    • (2003) Saccharomyces Cerevisiae , vol.230 , pp. 99-108
    • Bulter, T.1    Sieber, V.2    Alcalde, M.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.