Induced cytokine response of human PMBC-cultures: Correlation of gene expression and secretion profiling and the effect of cryopreservation

Cellular Immunology

Volumn 272, Issue 2, 2012, Pages 144-153

  Radke, Lars ^a   López Hemmerling, Diana A ^a   Lubitz, Annika ^b   Giese, Christoph ^b   Frohme, Marcus ^a  

^a Technische und forschende Hochschule Wildau   (Germany)

^b PROBIOGEN AG   (Germany)

Author keywords

Bead based multiplex assay; Cryopreservation; Cytokine; MRNA profiling; PBMC stimulation; Protein profiling

Indexed keywords

CONCANAVALIN A; GAMMA INTERFERON; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 12; INTERLEUKIN 13; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; MESSENGER RNA; OKT 3; POKEWEED MITOGEN; STAPHYLOCOCCUS ENTEROTOXIN B; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; CRYOPRESERVATION; CYTOKINE PRODUCTION; CYTOKINE RELEASE; CYTOKINE RESPONSE; FREEZE THAWING; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; IMMUNE RESPONSE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CONCANAVALIN A; CRYOPRESERVATION; ENTEROTOXINS; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; IMMUNOLOGIC FACTORS; INTERFERON-GAMMA; INTERLEUKIN-2; INTERLEUKIN-4; LEUKOCYTES, MONONUCLEAR; MUROMONAB-CD3; POKEWEED MITOGENS; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA;

EID: 83955162316     PISSN: 00088749     EISSN: 10902163     Source Type: Journal    
DOI: 10.1016/j.cellimm.2011.10.018     Document Type: Article

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