Primer design and transcript quantification of a highly multiplexed RT-PCR for a nonmodel avian species

Molecular Ecology Resources

Volumn 12, Issue 1, 2012, Pages 116-122

  Balenger, Susan L ^a   McClure, Christopher J W ^a   Hill, Geoffrey E ^a  

^a AUBURN UNIVERSITY   (United States)

Author keywords

Carpodacus mexicanus; Gene expression; House finch; Standard curve estimator

Indexed keywords

PRIMER DNA; TRANSCRIPTOME;

ANIMAL; ARTICLE; COMPUTER PROGRAM; GENETICS; MALE; MOLECULAR GENETICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SONGBIRD;

ANIMALS; DNA PRIMERS; MALE; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SOFTWARE; SONGBIRDS; TRANSCRIPTOME;

AVES; CARPODACUS MEXICANUS; PASSERI;

EID: 83955161740     PISSN: 1755098X     EISSN: 17550998     Source Type: Journal    
DOI: 10.1111/j.1755-0998.2011.03058.x     Document Type: Article

References (14)

1. Akaike H (1974) A new look at the statistical model identification. IEEE Transactions on Automatic Control, 19, 716-723.
2. Bonneaud C, Balenger SL, Russell AF, Zhang J, Hill GE, Edwards SV (2011) Rapidly evolving disease resistance manifest through functional changes in gene expression in a wild bird. Proceedings of the National Academy of Sciences of the United States of America, 108, 7866-7871.
3. Brisson M, Hall S, Hamby RK, Park R, Srere HK (2004) Optimization of single and multiplex real-time PCR. In: A-Z of Quantitative PCR (ed. Bustin SA), pp. 619-642. International University Line, La Jolla, California.
4. Burnham KP, Anderson DR (2002) Model Selection and Multimodel Inference: A Practical Information-Theoretic Approach, pp. 488. Springer-Verlag, New York.
5. Chen QR, Vansant G, Oades K et al. (2007) Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Journal of Molecular Diagnostics, 9, 80-88.
6. Hoffmann AA, Willi Y (2008) Detecting genetic responses to environmental change. Nature Reviews Genetics, 9, 421-432.
7. Nagel MA, Gilden D, Shade T, Gao B, Cohrs RJ (2009) Rapid and sensitive detection of 68 unique varicella zoster virus gene transcripts in five multiplex reverse-transcription-polymerase chain reactions. Journal of Virological Methods, 157, 62-68.
8. Piertney SB, Webster LMI (2010) Characterising functionally important and ecologically meaningful genetic diversity using a candidate gene approach. Genetica, 138, 419-432.
9. Rai AJ, Kamath RM, Gerald W, Fleisher M (2009) Analytical validation of the GeXP analyzer and design of workflow for cancer-biomarker discovery using multiplexed gene-expression profiling. Analytical & Bioanalytical Chemistry, 393, 1505-1511.
10. Shiu SH, Borevitz JO (2008) The next generation of microarray research: applications in evolutionary and ecological genomics. Heredity, 100, 141-149.
11. Therianos S, Zhu M, Pyun E, Coleman PD (2004) Single-channel quantitative multiplex reverse transcriptase-polymerase chain reaction for large numbers of gene products differentiates nondemented from neuropathological Alzheimer's disease. American Journal of Pathology, 164, 795-806.
12. Ungerer MC, Johnson LC, Herman MA (2008) Ecological genomics: understanding gene and genome function in the natural environment. Heredity, 100, 178-183.
13. Vasemägi A, Primmer CR (2005) Challenges for identifying functionally important genetic variation: the promise of combining complementary research strategies. Molecular Ecology, 14, 3623-3642.
14. Wang Z, Farmer KL, Hill GE, Edwards SV (2006) A cDNA macroarray approach to parasite-induced gene expression in a songbird host: genetic response of House Finches to experimental infection by Mycoplasma gallisepticum. Molecular Ecology, 15, 1263-1273.