Production and purification of a PER.C6-expressed IgM antibody therapeutic

BioProcess International

Volumn 9, Issue 11, 2011, Pages 28-37

  Valasek, Christopher ^a   Cole, Joshua ^a   Hensel, Frank ^b   Ye, Pei ^a   Conner, Michael A ^b   Ultee, Michiel E ^a  

^a Laureate Biopharmaceutical Services Inc   (United States)

^b Patrys Ltd   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN M ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; BIOREACTOR; CANCER THERAPY; CELL CULTURE; CELL GROWTH; CELL LINE; CHROMATOGRAPHY; HUMAN; NONHUMAN; PURIFICATION;

EID: 83755207401     PISSN: 15426319     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Article

References (15)

1. Reichert JM, Valge-Archer VE. Development Trends for Monoclonal Antibody Cancer Therapeutics. Nat. Rev. 6, 2007: 349-356. (Pubitemid 46696550)
2. Vollmers HP, Brandlein S. Natural Human Immunoglobulins in Cancer Immunotherapy. Immunother. 1(2) 2009: 241-248.
3. Janeway C. Approaching the Asymptote? Evolution and Revolution in Immunology. Cold Spring Harbor Symposium of Quantitative Biology 1989, 54:Part 1. Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, 1989; 1-13.
4. Medzhitov R. Toll-Like Receptors and Innate Immunity. Nat. Rev. Immunol. 1, 2001: 135-145. (Pubitemid 33741967)
5. Vollmers HP, Brandlein S. Nature's Best Weapons to Fight Cancer: Revival of Human Monoclonal Antibodies. Human Antibodies 11, 2002: 131-142. (Pubitemid 36470165)
6. Vollmers HP, Brandlein S. Natural IgM Antibodies, the Ignored Weapons in Tumor Immunity. Histol. Histopathol. 19, 2004: 897-905. (Pubitemid 38855071)
7. Vollmers HP, Brandlein S. Death By Stress: Natural IgM-Induced Apoptosis. Meth. Findings Experimental Clinical Pharmacol. 27, 2005: 185-191. (Pubitemid 40571213)
8. Hensel F, Gagnon P. An Effective Platform for Purification of IgM Monoclonal Antibodies. 5th International Conference on Hydroxyapatite and Related Products (Rottach-Egern, Germany, 11-14 October 2009). Dechema EV: Frankfurt Am Main, Germany.
9. Gagnon P, Hensel F, Richieri R. Purification of IgM Monoclonal Antibodies. BioPharm Int. March 2008; S26-S36.
10. Tchoudakova A, et al. High-Level Expression of Functional Human IgMs in Human PER.C6 Cells. MAbs 1(2) 2009: 163-171.
11. Cattaneo A, Neuberger MS. Polymeric Immunoglobulin M Is Secreted By Transfectants of Non Lymphoid Cells in the Absence of Immunoglobulin J Chain. EMBO J. 6, 1987: 2753-2758.
12. Cacciuttolo MA, et al. Large-Scale Production of a Monoclonal IgM in a Hybridoma Suspension Culture. BioPharm 20(9) 1998.
13. Lee J, et al. Adaptation of Hybridomas to Protein-Free Media Results in a Simplified Two-Step Immunoglobulin M Purification Process. J. Chromatogr. A 1216, 2009: 2683-2688.
14. Gagnon P, Hensel F, Richieri R. IgM Purification, the Next Generation. 13th Annual Waterside Conference (Miami, FL, 4-6 February 2008). Williamsburg Bioprocessing Foundation, www.wilbio.com.
15. Gagnon P, Hensel F. An Effective Platform for Purification of IgM Monoclonal Antibodies Using Hydroxyapatite. 5th International Conference on Hydroxyapatite and Related Products (Rottach-Egern, Germany, 11-14 October 2009). Dechema EV: Frankfurt Am Main, Germany.