Real-time reverse transcription polymerase chain reaction to measure mRNA: Use, limitations, and presentation of results

Anatomical Record

Volumn 295, Issue 1, 2012, Pages 1-3

  Jensen, Ellen C ^a  

^a NONE   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA;

EDITORIAL; GENE EXPRESSION; NOMENCLATURE; NORTHERN BLOTTING; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 83655161352     PISSN: 19328486     EISSN: 19328494     Source Type: Journal    
DOI: 10.1002/ar.21487     Document Type: Editorial

References (11)

1. Bustin SA. 2000. Absolute quantification of mRNA using real-time reverse transcription PCR assays. J Mol Endocrinol 25: 169-193.
2. Bustin SA, Benes V, Garson JA, Hellemans J, Hugget J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Witter CT. 2009. The MIQE guidelines: Minimum Information for publication of Quantitative real-time PCR Experiments. Clin Chem 55: 1-12.
3. Cai JH, Deng H, Kumpf SW, Lee PA, Zagouras P, Ryan A, Gallagher DS. 2007. Validation of rat reference genes for improved quantitative gene expression analysis using low density arrays. BioTechniques 42: 503-512.
4. Cale JM, Millican DS, Itoh, H, Magness RR, Bird IM. 1997. Pregnancy induces an increase in the expression of glyceraldehyde-3-phosphate-dehydrogenase in uterine artery endothelial cells. J Soc Gynecol Investig 4: 284-292.
5. Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A. 2004. Validation of house-keeping genes for normalizing RNA expression in real-time PCR. BioTechniques 37: 112-119.
6. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25: 402-408.
7. Mackay IM, Arden EK, Nitsche A. 2002. Real-time PCR in virology Nucleic Acids Res 30: 1292-1305.
8. Mansur NR, Meyer-Siegler K, Wurzer JC, Sirover MA. 1993. Cell cycle regulation of the glyceraldehyde-3-phosphate-dehydrogenase/uracil DNA glycosylase gene in normal human cells. Nucleic Acids Res 21: 993-998.
9. T method. Nat Protoc 3: 1101-1108.
10. Udvardi MK, Czechowski T, Scheible WR. 2008. Eleven golden rules of quantitative RT-PCR. Plant Cell 20: 1736-1737.
11. VanGuilder HD, Vrana KE, Freeman WM. 2008. Twenty-five years of quantitative PCR for gene expression analysis. BioTechniques 44: 619-626.