Fluorescence detection methods for microfluidic droplet platforms

Journal of Visualized Experiments

Volumn , Issue 58, 2011, Pages

  Casadevall i Solvas, Xavier ^a   Niu, Xize ^a   Leeper, Katherine ^a   Cho, Soongwon ^a   Chang, Soo Ik ^b   Edel, Joshua B ^a   deMello, Andrew J ^c  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b Chungbuk National University   (South Korea)

^c ETH ZURICH   (Switzerland)

Author keywords

Bioengineering; Droplet microfluidics; Fluorescence lifetime imaging; Fluorescence spectroscopy; Issue 58; Single cell assays; Single molecule assays

Indexed keywords

EID: 83455244357     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3437     Document Type: Article

References (11)

1. Whitesides, G.M. The origins and the future of microfluidics. Nature. 442, 368 (2006).
2. Zheng, B. & Ismagilov Angew, R.F. A Microfluidic Approach for Screening Submicroliter Volumes against Multiple Reagents by Using Preformed Arrays of Nanoliter Plugs in a Three-Phase Liquid/Liquid/Gas Flow. Chem. Int. Ed. 44, 2520 (2005).
3. Huebner, A., Sharma, S., Srisa-Art, M., Hollfelder, F., Edel, J.B., & Demello, A.J. Microdroplets: A sea of applications? Lab on a Chip. 8, 1244 (2008).
4. deMello, A.J. Control and detection of chemical reactions in microfluidic systems. Nature. 442, 394 (2006).
5. http://mems.gatech.edu/msmawebsite/members/processes/processes_files/SU8/Data%20Sheet%2050-100.pdf
6. Thorsen, T., Roberts, R.W., Arnold, F.H., & Quake, S.R. Dynamic pattern formation in a vesicle-generating microfluidic device. Phys. Rev. Lett. 86, 4163 (2001).
7. Anna, S.L., Bontoux, N., & Stone, H.A. Formation of dispersions using flow focusing in microchannels. Appl. Phys. Lett. 82, 364 (2003).
8. Casadevall i Solvas, X., Srisa-Art, M., deMello, A.J. & Edel, B.J. Mapping of Fluidic Mixing in Microdroplets with 1 μs Time Resolution Using Fluorescence Lifetime Imaging. Anal. Chem. 82, 3950 (2010).
9. Robinson, T.A., Schaerli, Y., Wootton, R., Hollfelder, F., Dunsby, C., Baldwin, G., Neil, M., French, P., & deMello, A.J. Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab. Chip. 9, 3437-3441 (2009).
10. Huebner, A., Srisa-Art, M., Holt, D., Abell, C., Hollfelder, F., deMello, A.J., & Edel, J.B. Quantitative detection of protein expression in single cells using droplet microfluidics. Chem. Commun. 1218 (2007).
11. Edel, J.B, Eid, J.S. & Meller, A. Accurate single molecule FRET efficiency determination for surface immobilized DNA using maximum likelihood calculated lifetimes. J. Phys. Chem. B. 73, 2986-2990 (2007).