Building a double hexamer of DNA helicase at eukaryotic replication origins

EMBO Journal

Volumn 30, Issue 24, 2011, Pages 4853-4855

  Labib, Karim ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; HELICASE; MINICHROMOSOME MAINTENANCE PROTEIN 2; MINICHROMOSOME MAINTENANCE PROTEIN 7; REPLICATION LICENSING FACTOR CDT1;

ARTICLE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; DNA REPLICATION; ENZYME ACTIVE SITE; EUKARYOTE; PRIORITY JOURNAL;

CELL CYCLE PROTEINS; DNA HELICASES; DNA REPLICATION; DNA-BINDING PROTEINS; REPLICATION ORIGIN; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 83455213558     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.1038/emboj.2011.437     Document Type: Article

References (11)

1. Chen S, Bell SP (2011) CDK prevents Mcm2-7 helicase loading by inhibiting Cdt1 interaction with Orc6. Genes Dev 25: 363-372
2. Chen S, de Vries MA, Bell SP (2007) Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading. Genes Dev 21: 2897-2907 (Pubitemid 350133438)
3. Evrin C, Clarke P, Zech J, Lurz R, Sun J, Uhle S, Li H, Stillman B, Speck C (2009) A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. Proc Natl Acad Sci USA 106: 20240-20245
4. Fu YV, Yardimci H, Long DT, Ho TV, Guainazzi A, Bermudez VP, Hurwitz J, van Oijen A, Scharer OD, Walter JC (2011) Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase. Cell 146: 931-941
5. Gambus A, Jones RC, Sanchez-Diaz A, Kanemaki M, van Deursen F, Edmondson RD, Labib K (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat Cell Biol 8: 358-366
6. Gambus A, Khoudoli GA, Jones RC, Blow JJ (2011) MCM2-7 form double hexamers at licensed origins in Xenopus egg extract. J Biol Chem 286: 11855-11864
7. Labib K (2010) How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev 24: 1208-1219
8. Moyer SE, Lewis PW, Botchan MR (2006) Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Proc Natl Acad Sci USA 103: 10236-10241 (Pubitemid 44051150)
9. Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF (2009) Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. Cell 139: 719-730
10. Takara TJ, Bell SP (2011) Multiple Cdt1 molecules act at each origin to load replication-competent Mcm2-7 helicases. EMBO J 30: 4885-4896
11. Yardimci H, Loveland AB, Habuchi S, van Oijen AM, Walter JC (2010) Uncoupling of sister replisomes during eukaryotic DNA replication. Mol Cell 40: 834-840