Morphological analysis of Drosophila larval peripheral sensory neuron dendrites and axons using genetic mosaics

Journal of Visualized Experiments

Volumn , Issue 57, 2011, Pages

  Rezaul Karim, M ^a,b   Moore, Adrian W ^a  

^a RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

^b SAITAMA UNIVERSITY   (Japan)

Author keywords

Dendritic arborization neurons; Developmental biology; Drosophila; Flp out; Immunohistochemistry; Issue 57; Larvae; MARCM; Neuroscience; Peripheral nervous system; Sensory neuron

Indexed keywords

EID: 83455195380     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3111     Document Type: Article

References (38)

1. Grueber, W.B., Jan, L.Y., & Jan, Y.N. Tiling of the Drosophila epidermis by multidendritic sensory neurons. Development. 129, 2867-2878 (2002).
2. Crozatier, M., & Vincent, A. Control of multidendritic neuron differentiation in Drosophila: the role of Collier. Dev Biol. 315, 232-242 (2008).
3. Hattori, Y., Sugimura, K., & Uemura, T. Selective expression of Knot/Collier, a transcriptional regulator of the EBF/Olf-1 family, endows the Drosophila sensory system with neuronal class-specific elaborated dendritic patterns. Genes Cells. 12, 1011-1022 (2007).
4. Jinushi-Nakao, S., et al. Knot/Collier and cut control different aspects of dendrite cytoskeleton and synergize to define final arbor shape. Neuron. 56, 963-978 (2007).
5. Sugimura, K., Satoh, D., Estes, P., Crews, S., & Uemura, T. Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt. Neuron. 43, 809-822 (2004).
6. Li, W., Wang, F., Menut, L., & Gao, F.B. BTB/POZ-zinc finger protein abrupt suppresses dendritic branching in a neuronal subtype-specific and dosage-dependent manner. Neuron. 43, 823-834 (2004).
7. Zlatic, M., Landgraf, M., & Bate, M. Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system. Neuron 37, 41-51 (2003).
8. Grueber, W.B., Ye, B., Moore, A.W., Jan, L.Y., & Jan, Y.N. Dendrites of distinct classes of Drosophila sensory neurons show different capacities for homotypic repulsion. Curr Biol. 13, 618-626 (2003).
9. Grueber, W.B., Jan, L.Y., & Jan, Y.N. Different levels of the homeodomain protein cut regulate distinct dendrite branching patterns of Drosophila multidendritic neurons. Cell. 112, 805-818 (2003).
10. Moore, A.W., Jan, L.Y., & Jan, Y.N. hamlet, a binary genetic switch between single- and multiple- dendrite neuron morphology. Science. 297, 1355-1358 (2002).
11. Gao, F. B., Brenman, J. E., Jan, L. Y., & Jan, Y. N. Genes regulating dendritic outgrowth, branching, and routing in Drosophila. Genes Dev. 13, 2549-2561 (1999).
12. Corty, M. M., Matthews, B. J., & Grueber, W. B. Molecules and mechanisms of dendrite development in Drosophila. Development. 136, 1049-1061 (2009).
13. Moore, A. W. Intrinsic mechanisms to define neuron class-specific dendrite arbor morphology. Cell Adh. Migr. 2, 81-82 (2008).
14. Hughes, C. L., & Thomas, J. B. A sensory feedback circuit coordinates muscle activity in Drosophila. Mol. Cell. Neurosci. 35, 383-396 (2007).
15. Nishimura, Y., et al. Selection of Behaviors and Segmental Coordination During Larval Locomotion Is Disrupted by Nuclear Polyglutamine Inclusions in a New Drosophila Huntington's Disease-Like Model. J Neurogenet. 24, 194-206 (2010).
16. Song, W., Onishi, M., Jan, L.Y., & Jan, Y.N. Peripheral multidendritic sensory neurons are necessary for rhythmic locomotion behavior in Drosophila larvae. Proc. Natl. Acad. Sci. U. S. A. 104, 5199-5204 (2007).
17. Hwang, R.Y., et al. Nociceptive neurons protect Drosophila larvae from parasitoid wasps. Curr Biol. 17, 2105-2116 (2007).
18. Xiang, Y., et al. Light-avoidance-mediating photoreceptors tile the Drosophila larval body wall. Nature. 468, 921-926 (2010).
19. Cheng, L.E., Song, W., Looger, L.L., Jan, L.Y., & Jan, Y.N. The role of the TRP channel NompC in Drosophila larval and adult locomotion. Neuron. 67, 373-380 (2010).
20. Babcock, D.T., Landry, C., & Galko, M.J. Cytokine signaling mediates UV-induced nociceptive sensitization in Drosophila larvae. Curr Biol. 19, 799-806 (2009).
21. Hafer, N. & Schedl, P. Dissection of Larval CNS in Drosophila Melanogaster. J. Vis. Exp. (1), e85, DOI: 10.3791/85 (2006).
22. Grueber, W.B., et al. Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology. Development. 134, 55-64 (2007).
23. Merritt, D.J. & Whitington, P.M. Central projections of sensory neurons in the Drosophila embryo correlate with sensory modality, soma position, and proneural gene function. J Neurosci. 15, 1755-1767 (1995).
24. Blair, S.S. Genetic mosaic techniques for studying Drosophila development. Development. 130, 5065-5072 (2003).
25. Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron. 22, 451-461 (1999).
26. Wong, A. M., Wang, J. W., & Axel, R. Spatial representation of the glomerular map in the Drosophila protocerebrum. Cell. 109, 229-241 (2002).
27. Shimono, K. et al. Multidendritic sensory neurons in the adult Drosophila abdomen: origins, dendritic morphology, and segment- and age-dependent programmed cell death. Neural Dev. 4, 37 (2009).
28. Feng, Y., Ueda, A., & Wu, C.F. A modified minimal hemolymph-like solution, HL3.1, for physiological recordings at the neuromuscular junctions of normal and mutant Drosophila larvae. J Neurogenet. 18, 377-402 (2004).
29. Sullivan, W., Ashburner, M., & Hawley, R.S. Drosophila Protocols. (Cold Spring Harbor Laboratory Press, 2000).
30. Kaczynski, T.J. & Gunawardena, S. Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos. J. Vis. Exp. (46), e2150, DOI: 10.3791/2150 (2010).
31. Featherstone, D.E., Chen, K., & Broadie, K. Harvesting and preparing Drosophila embryos for electrophysiological recording and other procedures. J Vis Exp. (2009).
32. Medina, P.M., Swick, L.L., Andersen, R., Blalock, Z., & Brenman, J.E. A novel forward genetic screen for identifying mutations affecting larval neuronal dendrite development in Drosophila melanogaster. Genetics. 172, 2325-2335 (2006).
33. Mirouse, V., Swick, L.L., Kazgan, N., St Johnston, D., & Brenman, J.E. LKB1 and AMPK maintain epithelial cell polarity under energetic stress. J Cell Biol. 177, 387-392 (2007).
34. Brent, J., Werner, K., & McCabe, B.D. Drosophila Larval NMJ Immunohistochemistry. J. Vis. Exp. (25), e1108, DOI: 10.3791/1108 (2009).
35. Brand, A.H. & Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 118, 401-415 (1993).
36. Sugimura, K., et al. Distinct developmental modes and lesion-induced reactions of dendrites of two classes of Drosophila sensory neurons. J Neurosci. 23, 3752-3760 (2003).
37. Zito, K., Parnas, D., Fetter, R.D., Isacoff, E.Y., & Goodman, C.S. Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila. Neuron. 22, 719-729 (1999).
38. Landgraf, M., Sanchez-Soriano, N., Technau, G.M., Urban, J., & Prokop, A. Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites. Dev Biol. 260, 207-225 (2003).