Cryopreservation of mouse embryos by ethylene glycol-based vitrification

Journal of Visualized Experiments

Volumn , Issue 57, 2011, Pages

  Mochida, Keiji ^a   Hasegawa, Ayumi ^a   Taguma, Kyuichi ^a   Yoshiki, Atsushi ^a   Ogura, Atsuo ^a  

^a RIKEN BIORESOURCE CENTER   (Japan)

Author keywords

Cryopreservation; Developmental biology; Embryo; Ethylene glycol; Issue 57; Mouse; Vitrification

Indexed keywords

EID: 83455163647     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3155     Document Type: Article

References (10)

1. Whittingham, D.G., Leibo, S.P., & Mazur, P. Survival of mouse embryos frozen to -196° and -269°C. Science. 187, 411-414 (1972).
2. Rall, W.F. & Fahy, G.M. Ice-free cryopreservation of mouse embryos at -196°C by vitrification. Nature. 313, 573-575 (1985).
3. Yoshiki, A., et al. The mouse resources at the RIKEN BioResource center. Exp. Anim. 58, 85-96 (2009).
4. Mochida, K. & Ogura, A. Cryopreservation of embryos in laboratory species. J. Mamm. Ova. Res. 27, 87-92 (2010).
5. Kasai, M., et al. A simple method for mouse embryos cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fert. 89, 91-97 (1990).
6. Dinnyes, A., Wallace, G.A., & Rall, W.F. Effect of genotype on the efficiency of mouse embryo cryopreservation by vitrification or slow freezing methods. Mol. Reprod. Dev. 40, 429-435 (1995).
7. Kasai, M. & Mukaida, T. Cryopreservation of animal and human embryos by vitrification. Reprod. Biomed. Online. 9, 164-70 (2004).
8. Miyake, T., et al. Vitrification of mouse oocytes and embryos at various stages of development in an ethylene glycol-based solution by a simple method. Theriogenology. 40, 121-134 (1993).
9. Han, M.S., Niwa, K., & Kasai, M. Vitrification of rat embryos at various developmental stages. Theriogenology. 59, 1851-1863 (2003).
10. Jin, B., et al. Equilibrium vitrification of mouse embryos. Biol. Reprod. 82, 444-450 (2010).