Dynamic instability in a DNA-segregating prokaryotic actin homolog

Science

Volumn 306, Issue 5698, 2004, Pages 1021-1025

  Garner, Ethan C ^a   Campbell, Christopher S ^a   Mullins, R Dyche ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; CHROMOSOMES; DNA; ENERGY TRANSFER; FLUORESCENCE; HYDROLYSIS; LIGHT REFLECTION; MICROSCOPIC EXAMINATION; POLYMERIZATION;

DNA SEGREGATION; EUKARYOTIC MICROTUBULES;

CYTOLOGY;

ACTIN; ADENOSINE TRIPHOSPHATE; DNA; MONOMER; POLYMER; TUBULIN;

BIOLOGY;

ACTIN FILAMENT; ACTIN POLYMERIZATION; ARTICLE; CELL FUNCTION; CHROMOSOME SEGREGATION; ENERGY TRANSFER; EVOLUTIONARY HOMOLOGY; FLUORESCENCE MICROSCOPY; HYDROLYSIS; MICROTUBULE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROKARYOSIS;

ACTINS; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEINS; BIOPOLYMERS; DNA, BACTERIAL; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS; KINETICS; MICROSCOPY, FLUORESCENCE; MUTAGENESIS;

EUKARYOTA; PROKARYOTA;

EID: 8344247018     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1101313     Document Type: Article

References (32)

1. T. Kruse, J. Møller-Jensen, A. Løbner-Olesen, K. Gerdes, EMBO J. 22, 5283 (2003).
2. H. J. Soufo, P. L. Graumann, Curr. Biol. 13, 1916 (2003).
3. Z. Gitai, N. Dye, L. Shapiro, Proc. Natl. Acad. Sci. U.S.A. 101, 8643 (2004).
4. K. Nordström, L. C. Ingram, A. Lundbäck, J. Bacteriol. 110, 562 (1972).
5. K. Gerdes, S. Molin, J. Mol. Biol. 190, 269 (1986).
6. K. Gerdes, J. E. Larsen, S. Molin, J. Bacteriol. 161, 292 (1985).
7. J. Møller-Jensen, R. B. Jensen, J. Lowë, K. Gerdes, EMBO J. 21, 3119 (2002).
8. J. Møller-Jensen et al., Mol. Cell 12, 1477 (2003).
9. M. Dam, K. Gerdes, J. Mol. Biol. 236, 1289 (1994).
10. F. van den Ent, J. Møller-Jensen, L. A. Amos, K. Gerdes, J. Lowë, EMBO J. 21, 6935 (2002).
11. See supporting data on Science Online.
12. T. Mitchison, M. Kirschner, Nature 312, 237 (1984).
13. H. Wendel, P. Dancker, Biochim. Biophys. Acta 915, 199 (1987).
14. E. Nishida, H. Sakai, J. Biochem. (Tokyo) 93, 1011 (1983).
15. F. Oosawa, M. Kasai, J. Mol. Biol. 4, 10 (1962).
16. E. M. Mandelkow, G. Lange, A. Jagla, U. Spann, E. Mandelkow, EMBO J. 7, 357 (1988).
17. M. F. Carlier, R. Melki, D. Pantaloni, T. L. Hill, Y. Chen, Proc. Natl. Acad. Sci. U.S.A. 84, 5257 (1987).
18. E. C. Garner, C. S. Campbell, R. D. Mullins, data not shown.
19. C. Combeau, M. F. Carlier, J. Biol. Chem. 263, 17429 (1988).
20. L. Blanchoin, T. D. Pollard, Biochemistry 41, 597 (2002).
21. L. Romberg, T. J. Mitchison, Biochemistry 43, 282 (2004).
22. S. Vorobiev et al., Proc. Natl. Acad. Sci. U.S.A. 100, 5760 (2003).
23. D. Panda, H. P. Miller, L. Wilson, Biochemistry 41, 1609 (2002).
24. D. N. Drechsel, M. W. Kirschner, Curr. Biol. 4, 1053 (1994).
25. V. K. Vinson, E. M. De La Cruz, H. N. Higgs, T. D. Pollard, Biochemistry 37, 10871 (1998).
26. M. D. Welch, R. D. Mullins, Annu. Rev. Cell Dev. Biol. 18, 247 (2002).
27. S. H. Zigmond, Curr. Opin. Cell Biol. 16, 99 (2004).
28. M. Pring, M. Evangelista, C. Boone, C. Yang, S. H. Zigmond, Biochemistry 42, 486 (2003).
29. T. J. Mitchison, E. D. Salmon, Nature Cell Biol. 3, E17 (2001).
30. R. B. Jensen, K. Gerdes, J. Mol. Biol. 269, 505 (1997).
31. R. B. Jensen, K. Gerdes, EMBO J. 18, 4076 (1999).
32. We thank members of the Mullins lab for moral support and helpful discussions; L. Frost for R1 plasmid; N. Stuurman and A. Douglas for invaluable assistance with TIRF microscopy; M. Tanegawa for advice on FRET; and T. Mitchison, K. Ryan, R. Vale, M. Dayel, and Q. Justman for critical reading of the manuscript. Supported by NIH grant GM61010-01, Pew Charitable Trust grant P032SSC, and a grant from the Sandler Family Supporting Foundation (R.D.M.). Both E.C.G. and C.S.C. are supported by NSF Predortoral Fellowships. C.S.C. acknowledges B. Millie for continuing support and advice, and E.C.G. acknowledges B. Zakopyko for being triumphant.