An improved intracellular staining protocol for efficient detection of nuclear proteins in YFP-expressing cells

BioTechniques

Volumn 51, Issue 6, 2011, Pages 417-420

  Grupillo, Maria ^a,b,c   Lakomy, Robert ^a   Geng, Xuehui ^a   Styche, Alexis ^a   Rudert, William A ^a   Trucco, Massimo ^a   Fan, Yong ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b RiMED Foundation   (Italy)

^c UNIVERSITY OF PISA   (Italy)

Author keywords

And t regulatory cells; Flow cytometry; Foxp3; Intracellular staining; Membrane permeabilization; YFP

Indexed keywords

CELL NUCLEUS ANTIGEN; NUCLEAR PROTEIN; TRANSCRIPTION FACTOR FOXP3; YELLOW FLUORESCENT PROTEIN;

ARTICLE; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CYTOPLASM; FLOW CYTOMETRY; GENETIC TRANSDUCTION; HUMAN; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REGULATORY T LYMPHOCYTE; SIGNAL TRANSDUCTION; SPLEEN CELL; STAINING; TRANSGENIC MOUSE;

ANIMALS; BACTERIAL PROTEINS; CELL MEMBRANE PERMEABILITY; CYTOPLASM; LUMINESCENT PROTEINS; MICE; NUCLEAR PROTEINS; SPLEEN; STAINING AND LABELING; T-LYMPHOCYTES, REGULATORY; TISSUE FIXATION;

EID: 83255176240     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113780     Document Type: Article

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