Genetic background and mobility of variants of the gene nleA in attaching and effacing Escherichia coli

Applied and Environmental Microbiology

Volumn 77, Issue 24, 2011, Pages 8705-8713

  Creuzburg, Kristina ^a,b   Heeren, Sabine ^a   Lis, Claudia M ^a   Kranz, Markus ^a   Hensel, Michael ^b   Schmidt, Herbert ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

^b UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SEQUENCING; EFFECTOR GENES; ENTEROPATHOGENIC ESCHERICHIA COLI; FLANKING REGIONS; GENE ORDERS; GENETIC BACKGROUNDS; PROPHAGES; REFERENCE STRAINS; SEROGROUPS;

ENCODING (SYMBOLS); ESCHERICHIA COLI; POLYMERASE CHAIN REACTION;

GENE ENCODING;

ADHESIN; BACTERIAL DNA; EAEA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; NLEA PROTEIN, E COLI; VIRULENCE FACTOR;

BACTERIOPHAGE; COLIFORM BACTERIUM; GENETIC ANALYSIS; HYBRIDIZATION; MOBILITY; MORPHOGENESIS; PATHOGENICITY; POLYMERASE CHAIN REACTION; PROTEIN; TOXIN;

ARTICLE; CHEMISTRY; DNA SEQUENCE; ENTEROPATHOGENIC ESCHERICHIA COLI; GENE ORDER; GENETICS; HORIZONTAL GENE TRANSFER; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PROPHAGE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SYNTENY;

ADHESINS, BACTERIAL; DNA, BACTERIAL; ENTEROPATHOGENIC ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE ORDER; GENE TRANSFER, HORIZONTAL; MOLECULAR SEQUENCE DATA; PROPHAGES; SEQUENCE ANALYSIS, DNA; SHIGA-TOXIGENIC ESCHERICHIA COLI; SYNTENY; VIRULENCE FACTORS;

HONSHU; JAPAN; KINKI; SHIGA;

EID: 83155193001     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.06492-11     Document Type: Article

References (30)

1. Asadulghani, M., et al. 2009. The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinants. PLoS Pathog. 5:e1000408.
2. Buss, C., D. Müller, C. Rüter, G. Heusipp, and M. A. Schmidt. 2009. Identification and characterization of Ibe, a novel type III effector protein of A/E pathogens targeting human IQGAP1. Cell. Microbiol. 11:661-677.
3. Chang, A. C., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
4. Coombes, B. K., et al. 2008. Molecular analysis as an aid to assess the public health risk of non-O157 Shiga toxin-producing Escherichia coli strains. Appl. Environ. Microbiol. 74:2153-2160.
5. Creuzburg, K., et al. 2011. Evolutionary analysis and distribution of type III effector genes in pathogenic Escherichia coli from human, animal and food sources. Environ. Microbiol. 13:439-452.
6. Creuzburg, K., et al. 2005. The Shiga toxin 1-converting bacteriophage BP- 4795 encodes an NleA-like type III effector protein. J. Bacteriol. 187:8494-8498.
7. Creuzburg, K., and H. Schmidt. 2007. Molecular characterization and distribution of genes encoding members of the type III effector NleA family among pathogenic Escherichia coli strains. J. Clin. Microbiol. 45:2498-2507.
8. Garmendia, J., G. Frankel, and V. F. Crepin. 2005. Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation. Infect. Immun. 73:2573-2585.
9. Gruenheid, S., et al. 2004. Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157:H7. Mol. Microbiol. 51:1233-1249.
10. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
11. Hayashi, T., et al. 2001. Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 8:11-22.
12. Hendrix, R. W., J. G. Lawrence, G. F. Hatfull, and S. Casjens. 2000. The origins and ongoing evolution of viruses. Trends Microbiol. 8:504-508.
13. Iguchi, A., et al. 2009. Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69. J. Bacteriol. 191:347-354.
14. Kim, J., et al. 2007. The bacterial virulence factor NleA inhibits cellular protein secretion by disrupting mammalian COPII function. Cell Host Microbe 2:160-171.
15. Lee, S. F., et al. 2008. A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. Cell. Microbiol. 10:499-513.
16. Loukiadis, E., et al. 2008. Distribution, functional expression, and genetic organization of Cif, a phage-encoded type III-secreted effector from enteropathogenic and enterohemorrhagic Escherichia coli. J. Bacteriol. 190:275-285.
17. Lukashin, A. V., and M. Borodovsky. 1998. GeneMark.hmm: new solutions for gene finding. Nucleic Acids Res. 26:1107-1115.
18. Mundy, R., et al. 2004. Identification of a novel Citrobacter rodentium type III secreted protein, EspI, and roles of this and other secreted proteins in infection. Infect. Immun. 72:2288-2302.
19. Nataro, J. P., and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201.
20. Ogura, Y., et al. 2008. Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of whole-genome PCR scanning and fosmid mapping. J. Bacteriol. 190:6948-6960.
21. Ogura, Y., et al. 2009. Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 106:17939-17944.
22. Perna, N. T., et al. 2001. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409:529-533.
23. Pridmore, R. D. 1987. New and versatile cloning vectors with kanamycinresistance marker. Gene 56:309-312.
24. Recktenwald, J., and H. Schmidt. 2002. The nucleotide sequence of Shiga toxin (Stx) 2e-encoding phage P27 is not related to other Stx phage genomes, but the modular genetic structure is conserved. Infect. Immun. 70: 1896-1908.
25. Schmidt, H., M. Bielaszewska, and H. Karch. 1999. Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage 538 isolated from Escherichia coli O157:H7. Appl. Environ. Microbiol. 65:3855-3861.
26. Stavrinides, J., H. C. McCann, and D. S. Guttman. 2008. Host-pathogen interplay and the evolution of bacterial effectors. Cell. Microbiol. 10:285-292.
27. Thanabalasuriar, A., et al. 2010. The bacterial virulence factor NleA is required for the disruption of intestinal tight junctions by enteropathogenic E. coli. Cell. Microbiol. 12:31-41.
28. Tobe, T., et al. 2006. An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination. Proc. Natl. Acad. Sci. U. S. A. 103:14941-14946.
29. Wong, A. R., et al. 2011. Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements. Mol. Microbiol. 80:1420-1438.
30. Zhou, Z., et al. 2010. Derivation of Escherichia coli O157:H7 from its O55:H7 precursor. PLoS One 5:e8700.