Extracellular proteinase formation in carbon starving Aspergillus nidulans cultures - Physiological function and regulation

Journal of Basic Microbiology

Volumn 51, Issue 6, 2011, Pages 625-634

  Szilágyi, Melinda ^a   Kwon, Nak Jung ^b   Bakti, Fruzsina ^a   M Hamvas, Márta ^a   Jámbrik, Katalin ^a   Park, Heesoo ^b   Pócsi, István ^a   Yu, Jae Hyuk ^b   Emri, Tamás ^a  

^a UNIVERSITY OF DEBRECEN   (Hungary)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Aspergillus nidulans; Autolysis; Extracellular proteinase; FluG BrlA signaling; Heterologous protein production

Indexed keywords

CARBON; PEPTIDE HYDROLASE;

ARTICLE; ASPERGILLUS NIDULANS; AUTOLYSIS; CHEMISTRY; CULTURE MEDIUM; ENZYMOLOGY; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGY; SECRETION;

ASPERGILLUS NIDULANS; AUTOLYSIS; CARBON; CULTURE MEDIA; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; PEPTIDE HYDROLASES;

EID: 83155189208     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.201100068     Document Type: Article

References (48)

1. Sandhya, C., Sumantha, A., Pandey, A., 2005. Protease. In: Pandey, A., Webb, C., Soccol, C.R., Larroche, C. (eds.) Enzyme Technology. Asiatech Publishers Inc, New Delhi, pp. 319-332.
2. Donlon, J., 2007. Subtilisin. In: Polaina, J., MacCabe, A.P. (eds.), Industrial Enzymes. Dordrecht: Springer, pp. 197-206.
3. Sumantha, A., Larroche, C., Pandey, A., 2006. Microbiology and industrial biotechnology of food-grade proteases: a perspective. Food Technol. Biotechnol., 44, 211-220.
4. Terpe, K., 2006. Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol., 72, 211-222.
5. Lubertozzi, D., Keasling, J.D., 2009. Developing Aspergillus as a host for heterologous expression. Biotechnol. Adv., 27, 53-75.
6. de Vries, R.P., Burgers, K., van de Vondervoort, P. J., Frisvad, J. C. et al. 2004. A new black Aspergillus species, A. vadensis, is a promising host for homologous and heterologous protein production. Appl. Environ. Microbiol., 70, 3954-3959.
7. Kimura, S., Maruyama, J., Takeuchi, M., Kitamoto, K., 2008. Monitoring global gene expression of proteases and improvement of human lysozyme production in the nptB gene disruptant of Aspergillus oryzae. Biosci. Biotechnol. Biochem., 72, 499-505.
8. Yoon, J., Kimura, S., Maruyama, J., Kitamoto, K., 2009. Construction of quintuple protease gene disruptant for heterologous protein production in Aspergillus oryzae. Appl. Microbiol. Biotechnol., 82, 691-701.
9. Ahamed, A., Singh, A., Ward, O.P., 2007. Chymostatin can combine with pepstatin to eliminate extracellular protease activity in cultures of Aspergillus niger NRRL-3. J. Ind. Microbiol. Biotechnol., 34, 165-169.
10. Wang, L.P., Ridgway, D., Gu, T.Y., Moo-Young, M., 2005. Bioprocessing strategies to improve heterologous protein production in filamentous fungal fermentations. Biotechnol. Adv., 23, 115-129.
11. Li, Q., Harvey, L.M., McNeil, B., 2008. The effects of bioprocess parameters on extracellular proteases in a recombinant Aspergillus niger B1-D. Appl. Microbiol. Biotechnol., 78, 333-341.
12. Denison, S. H., 2000. pH regulation of gene expression in fungi. Fungal. Genet. Biol., 29, 61-71.
13. Pusztahelyi, T., Pócsi, I., Kozma, J., Szentirmai, A., 1997. Ageing of Penicillium chrysogenum cultures under carbon starvation I: morphological changes and secondary metabolite production. Biotechnol. Appl. Biochem., 25, 81-88.
14. Emri, T., Molnár, Zs., Pusztahelyi, T., Pócsi, I., 2004. Physiological and morphological changes in autolysing Aspergillus nidulans cultures. Folia Microbiol., 49, 277-284.
15. McIntyre, M., Berry, D.R., McNeil, B., 2000. Role of proteases in autolysis of Penicillium chrysogenum chemostat cultures in response to nutrient depletion. Appl. Microbiol. Biotechnol., 53, 235-242.
16. Emri, T., Molnár, Zs., Veres, T., Pusztahelyi, T. et al., 2006. Glucose mediated repression of autolysis and conidiogenesis in Emericella nidulans. Mycol. Res., 110, 1172-1178.
17. Emri, T., Molnár, Zs., Szilágyi, M., Pócsi, I., 2008. Regulation of autolysis in Aspergillus nidulans. Appl. Biochem. Biotechnol., 151, 211-220.
18. Emri, T., Szilágyi, M., László, K., Hamvas, M., Pócsi, I., 2009. PepJ is a new extracellular protease of Aspergillus nidulans. Folia Microbiol., 54, 105-109.
19. Peña-Montes, C., González, A., Castro-Ochoa, D., Farrés, A., 2008. Purification and biochemical characterization of a broad substrate specificity thermostable alkaline protease from Aspergillus nidulans. Appl. Microbiol. Biotechnol., 78, 603-612.
20. Fujinami, S., Fujisawa, M., 2010. Industrial applications of alkaliphiles and their enzymes - past, present and future. Environ. Technol., 31, 845-856.
21. Chi, Z., Chi, Z., Zhang, T., Liu, G. et al., 2009. Production, characterization and gene cloning of the extracellular enzymes from the marine-derived yeasts and their potential applications. Biotechnol. Adv., 27, 236-255.
22. Ueda, M., Kubo, T., Miyatake, K., Nakamura, T., 2007. Purification and characterization of fibrinolytic alkaline protease from Fusarium sp. BLB. Appl. Microbiol. Biotechnol., 74, 331-338.
23. Pócsi, I., Leiter, É., Kwon, N.J., Shin, K.S. et al., 2009. Asexual sporulation signaling regulates autolysis of Aspergillus nidulans via modulating the chitinase ChiB production. J. Appl. Microbiol., 107, 514-523.
24. Szilágyi, M., Kwon, N.J., Dorogi, Cs., Pócsi, I. et al., 2010. The extracellular β-1, 3-endoglucanase EngA is involved in autolysis of Aspergillus nidulans. J. Appl. Microbiol., 109, 1498-1508.
25. Shin, K.S., Kwon, N.J., Kim, Y.H., Park, H.S. et al., 2009. Differential roles of the ChiB chitinase in autolysis and cell death of Aspergillus nidulans. Eukaryot. Cell, 8, 738-746.
26. Emri, T., Szilágyi, M., Justyák, A., Pócsi, I., 2008. Heterotrimeric G protein mediated regulation of proteinase production in Aspergillus nidulans. Acta Microbiol. Immunol. Hung., 55, 111-117.
27. Katz, M.E., Evans, C.J., Heagney, E.E., van Kuyk, P.A. et al., 2009. Mutations in genes encoding sorting nexins alter production of intracellular and extracellular proteases in Aspergillus nidulans. Genetics, 181, 1239-1247.
28. Shroff, R.A., O'Connor, S.M., Hynes, M.J., Lockington, R.A., Kelly, J.M., 1997. Null alleles of creA, the regulator of carbon catabolite repression in Aspergillus nidulans. Fungal Genet. Biol., 22, 28-38.
29. Chang, M.H., Chae, K.S., Han, D.M., Jahng, K.Y., 2004. The GanB Galpha-protein negatively regulates asexual sporulation and plays a positive role in conidial germination in Aspergillus nidulans. Genetics, 167, 1305-1315.
30. Han, K.H., Seo, J.A., Yu, J.H., 2004. Regulators of G-protein signalling in Aspergillus nidulans: RgsA downregulates stress response and stimulates asexual sporulation through attenuation of GanB (Gα) signalling. Mol. Microbiol., 53, 529-540.
31. Rosén, S., Yu, J.H., Adams, T.H., 1999. The Aspergillus nidulans sfaD gene encodes a G protein beta subunit that is required for normal growth and repression of sporulation. EMBO J., 18, 5592-5600.
32. Seo, J.A., Han, K.H., Yu, J.H., 2005. Multiple roles of a heterotrimeric G-protein gamma-subunit in governing growth and development of Aspergillus nidulans. Genetics, 171, 81-89.
33. Kwon, N.J., Shin, K.S., Yu, J.H., 2010. Characterization of the developmental regulator FlbE in Aspergillus fumigatus and Aspergillus nidulans. Fungal Genet. Biol., 47, 981-993.
34. Tomarelli, R.M., Charney, J., Harding, M.L., 1949. The use of azoalbumin as a substrate in the colorimetric determination of peptic and tryptic activity. J. Lab. Clin. Med., 34, 428-433.
35. Yu, J.H., Hamari, Z., Han, K.H., Seo, J.A. et al., 2004. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol., 41, 973-981.
36. Emri, T., Molnár, Zs., Pusztahelyi, T., Varecza, Z., Pócsi, I., 2005. The FluG-BrlA pathway contributes to the initialisation of autolysis in submerged Aspergillus nidulans cultures. Mycol. Res., 109, 757-763.
37. Yu, J.H., 2006. Heterotrimeric G protein signaling and RGSs in Aspergillus nidulans. J. Microbiol., 44, 145-154.
38. Tilburn, J., Sarkar, S., Widdick, D.A., Espeso, E.A. et al., 1995. The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J., 14, 779-790.
39. Wong, K.H., Hynes, M.J., Todd, R.B., Davis, M.A., 2007. Transcriptional control of nmrA by the bZIP transcription factor MeaB reveals a new level of nitrogen regulation in Aspergillus nidulans. Mol. Microbiol., 66, 534-551.
40. Szilágyi, M., Pócsi, I., Forgács, K., Emri, T., 2010. MeaB dependent nutrition sensing regulates autolysis in carbon starving Aspergillus nidulans cultures. Ind. J. Microbiol., 50, 104-108.
41. Pusztahelyi, T., Pócsi, I., Szentirmai, A., 1997. Ageing of Penicillium chrysogenum cultures under carbon starvation. II. Protease and N-acetyl-β -D-hexosaminidase production. Biotechnol. Appl. Biochem., 25, 87-93.
42. Adams, T.H., Wieser, J.K., Yu, J.H., 1998. Asexual sporulation in Aspergillus nidulans. Microbiol. Mol. Biol., 62, 35-54.
43. Katz, M.E., Gray, K.A., Cheetham, B.F., 2006. The Aspergillus nidulans xprG (phoG) gene encodes a putative transcriptional activator involved in the response to nutrient limitation. Fungal Genet. Biol., 43, 190-199.
44. G203R and ΔflbA mutations on the morphology and physiology of submerged Aspergillus nidulans cultures. Appl. Biochem. Biotechnol., 118, 349-360.
45. Molnár, Zs., Emri, T., Zavaczki, E., Pusztehelyi, T., Pócsi, I., 2006. Effects of mutations in the GanB/RgsA G protein mediated signaling on the autolysis of Aspergillus nidulans. J. Basic Microbiol., 46, 495-603.
46. Broekhuijsen, M.P., Mattern, I.E., Contreras, R., Kinghorn, J.R., van den Hondel, C.A.M.J.J., 1993. Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein. J. Biotechnol., 31, 135-145.
47. Wiebe, M.G., Karandikar, A., Robson, G.D., Trinci, A.P. et al. 2001. Production of tissue plasminogen activator (t-PA) in Aspergillus niger. Biotechnol. Bioeng., 76, 164-174.
48. Mattern, I.E., van Noort J.M., van den Berg, P., Archer, D. B. et al., 1992. Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases. Mol. Gen. Genet., 234, 332-336.