CpG hypermethylation of the c-myc promoter by dsRNA results in growth suppression

Molecular Pharmaceutics

Volumn 8, Issue 6, 2011, Pages 2302-2309

  Mehndiratta, Mohit ^a,b   Palanichamy, Jayanth Kumar ^a   Pal, Arnab ^a,c   Bhagat, Mohita ^a   Singh, Anand ^a   Sinha, Subrata ^a,d   Chattopadhyay, Parthaprasad ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b UNIVERSITY COLLEGE OF MEDICAL SCIENCES   (India)

^c NORTH EASTERN INDIRA GANDHI REGIONAL INSTITUTE OF HEALTH AND MEDICAL SCIENCES   (India)

^d NATIONAL BRAIN RESEARCH CENTRE   (India)

Author keywords

c myc; cancer stem cells; DNA methylation; siRNA; transcriptional gene silencing

Indexed keywords

ACTIN; BETA ACTIN; DOUBLE STRANDED RNA; SMALL INTERFERING RNA;

ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CANCER STEM CELL; CARCINOGENESIS; CELL PROLIFERATION; CONTROLLED STUDY; CPG ISLAND; DOWN REGULATION; EPIGENETICS; GENE DOSAGE; GENE LOCUS; GENE OVEREXPRESSION; GENE SILENCING; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MARKER GENE; NANOG GENE; OCT 4 GENE; ONCOGENE; ONCOGENE C MYC; PRIORITY JOURNAL; PROMOTER REGION; SENESCENCE;

ANIMALS; BLOTTING, WESTERN; CACO-2 CELLS; CELL LINE, TUMOR; CELL PROLIFERATION; CPG ISLANDS; DNA METHYLATION; DOWN-REGULATION; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE SILENCING; GENES, MYC; HUMANS; PROMOTER REGIONS, GENETIC; RNA, DOUBLE-STRANDED;

EID: 82955240692     PISSN: 15438384     EISSN: 15438392     Source Type: Journal    
DOI: 10.1021/mp200177z     Document Type: Article

References (21)

1. Wierstra, I.; Alves, J. The c-myc promoter: still MysterY and Challenge Adv. Cancer Res. 2008, 99, 113-333 (Pubitemid 350137254)
2. Rich, J. N. Cancer stem cells in radiation resistance Cancer Res. 2007, 67, 8980-8984 (Pubitemid 47535877)
3. Wicha, M. S.; Liu, S.; Dontu, G. Cancer Stem Cells: An Old Idea-A Paradigm Shift Cancer Res. 2006, 66, 1883-1890
4. Fang, J. Y.; Xiao, S. D. Alteration in DNA methylation in gastrointestinal carcinogenesis J. Gastroenterol. Hepatol. 2001, 16, 960-968 (Pubitemid 32947136)
5. Fire, A.; Xu, S.; Montgomery, M. K.; Kostas, S. A.; Driver, S. E.; Mello, C. C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Nature 1998, 391, 806-811 (Pubitemid 28099681)
6. Mette, M. F.; Aufsatz, W.; Winden, J. V. D.; Matzke, M. A.; Matzke, A. J. M. Transcriptional silencing and promoter methylation triggered by double-stranded RNA EMBO J. 2000, 19, 5194-5201
7. Morris, K. V. RNA-Mediated Transcriptional Gene Silencing in Human Cells Curr. Top. Microbiol. Immunol. 2008, 320, 211-24
8. Palanichamy, J. K.; Mehndiratta, M.; Bhagat, M.; Ramalingam, P.; Das, B.; Das, P.; Sinha, S.; Chattopadhyay, P. Silencing of integrated human papillomavirus-16 oncogenes by small interfering RNA-mediated heterochromatization Mol. Cancer Ther. 2010, 9, 2114-22
9. Donze, O.; Picard, D. RNA interference in mammalian cells using siRNAs synthesised with T7 RNA polymerase Nucleic Acids Res. 2002, 30, e46
10. Vallot, C.; Stransky, N.; Bernard-Pierrot, I.; Hérault, A.; Zucman-Rossi, J.; Chapeaublanc, E.; Vordos, D.; Laplanche, A.; Benhamou, S.; Lebret, T.; Southgate, J; Allory, Y.; Radvanyi, F. A novel epigenetic phenotype associated with the most aggressive pathway of bladder tumor progression J. Natl. Cancer Inst. 2011, 103, 47-60
11. Salk Institute for Biological Studies, La Jolla, CA. Available at http://pingu.salk.edu/software.html.
12. Gary, R. K.; Kindell, S. M. Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts Anal. Biochem. 2005, 343, 329-34 (Pubitemid 41074047)
13. Bush, A.; Mateyak, M.; Dugan, K. c-myc null cells misregulate cad and gadd45 but not other proposed c-myc targets Genes Dev. 1998, 12, 3797-3802 (Pubitemid 29024841)
14. Clement, V.; Sanchez, P.; Tribolet, N.; Radovanovic, I.; Altaba, A. R. HEDGEHOG-GLI1 signaling regulates human glioma growth, cancer stem cell self-renewal and tumorigenicity Curr. Biol. 2007, 17, 165-172 (Pubitemid 46107823)
15. Fish, R. J.; Kruithof, E. K. O. Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors BMC Mol. Biol. 2004, 5, 9
16. Valdez, B. C.; Li, Y.; Murray, D.; Corn, P.; Champlin, R. E.; Andersson, B. S. 5-Aza-2′-deoxycytidine sensitizes busulfan-resistant myeloid leukemia cells by regulating expression of genes involved in cell cycle checkpoint and apoptosis Leuk. Res. 2010, 34, 364-72
17. Weinstein, I. B.; Joe, A. Oncogene addiction Cancer Res. 2008, 68, 3077-3080
18. Brooks, T. A.; Hurley, L. H. The role of supercoiling in transcriptional control of MYC and its importance in molecular therapeutics Nat. Rev. Cancer 2009, 9, 849-861
19. Bertrand, J. R.; Pottier, M.; Vekris, A.; Opolon, P.; Maksimenko, A.; Malvy, C. Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo Biochem. Biophys. Res. Commun. 2002, 296, 1000-1004
20. Wang, Y.; Liu, S.; Zhang, G.; Zhou, C. Q.; Zhu, H. X.; Zhou, X. B.; Quan, L. P.; Bai, J. F.; Xu, N. Z. Knockdown of c-Myc expression by RNAi inhibits MCF-7 breast tumor cells growth in vitro and in vivo Breast Cancer Res. 2005, 7, R220-R228
21. Napoli, S.; Pastori, C.; Magistri, M.; Carbone, G. M.; Catapano, C. V. Promoter-specific transcriptional interference and c-myc gene silencing by siRNAs in human cells EMBO J. 2009, 28, 1708-19