18F-FDG labelled leukocytes in vitro functional tests: Viability, chemotaxis and phagocytosis assays

Open Nuclear Medicine Journal

Volumn 3, Issue , 2011, Pages 25-29

  Lafont, Pannie ^a   Morelec, Isabelle ^a   Fraysse, Marc ^a   Got, Philippe ^a   Houzard, Claire ^a,b   Confavreux, Antoine ^a   Foucher, Antoine ^a   Giammarile, Francesco ^a,b  

^a CENTRE HOSPITALIER LYON SUD   (France)

^b UNIVERSITÉ LYON 1   (France)

Author keywords

18F FDG; Chemotaxis; Infection; Inflammation; Leukocytes; PET CT; Phagocytosis; Zymosan

Indexed keywords

AGAROSE; CHEMOATTRACTANT; FLUORODEOXYGLUCOSE F 18; TRYPAN BLUE; ZYMOSAN;

ADULT; ARTICLE; CELL ASSAY; CELL ISOLATION; CELL LABELING; CELL VIABILITY; CHEMOTAXIS; FEMALE; FUNCTIONAL ASSESSMENT; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; LEUKOCYTE FUNCTION; LEUKOCYTE MIGRATION; MALE; PHAGOCYTOSIS; PRIORITY JOURNAL; VOLUNTEER;

EID: 82955201634     PISSN: None     EISSN: 1876388X     Source Type: Journal    
DOI: 10.2174/1876388X01103010025     Document Type: Article

References (30)

1. 18F] fluoro-2-deoxy-D-glucose as a potential in vitro agent for labelling granulocytes for clinical studies by positron emission tomography. Int J Rad Appl Instrum B 1992; 19: 183-90.
2. Hoffman JM, Gamnhir SS. Molecular imaging: The vision and opportunity for radiology in the future. Radiology 2007; 244: 39-47.
3. Oehr P. Metabolism and transport of glucose and FDG. In: Ruhlmann J, Oehr P, Biersack HJ, Eds. Pet in Oncology: basics and clinical Application. Springer: Berlin 1999.
4. Saguwara Y, Braun DK, Kison PV, Russo JE, Zasadny KR, Wahl RL. Rapid detection of human infections with fluorine-18 fluorodeoxyglucose and positron-emission tomography: preliminary results. Eur J Nucl Med 1998; 25: 1238-43.
5. Bakheet SM, Saleem M, Powe J, et al. F-18-fluorodeoxyglucose chest uptake in lung inflammation and infection. Clin Nucl Med 2000; 25: 273-278.
6. Zhuang HA. 18-Fluorodeoxyglucose positron emission tomographic imaging in the detection and monitoring of infection and inflammation. Semin Nucl Med 2002; 32: 47-59.
7. Dadparvar S, Anderson GS, Bhargava P, et al. Paraneoplastic encephalitis associated with cystic teratoma is detected by fluorodeoxyglucose positron emission tomography with negative magnetic resonance image findings. Clin Nucl Med 2003; 28: 893-6.
8. De Winter F, Gemmel F, Van de Wiele C, et al. 18-Fluorine fluorodeoxyglucose positron emission tomography for the diagnosis of infection in the postoperative spine. Spine 2003; 28: 1314-9.
9. Yu JQ, Kumar R, Xiu Y et al. Diffuse FDG uptake in the lungs in aspiration pneumonia on positron emission tomographic imaging. Clin Nucl Med 2004; 29: 567-568.
10. Bleeker-Rovers CP, de Kleijn E, Corstens FHM, et al. Clinical value of FDG-PET in patients with fever of unknown origin and patients suspected of focal infection or inflammation. Eur J Nucl Med Mol Imaging 2004; 31: 29-37.
11. Yu JQ, Kung JW, Potenta S. et al. Chronic cholecystitis detected by FDG-PET. Clin Nucl Med 2004; 29: 496-7.
12. Rennen HJJM, Corstens FHM, Oyen WJG, Boerman OC. New concepts in infection/inflammation imaging. Q J Nucl Med 2001; 45: 167-73.
13. Jones HA, Schofield JB, Krausz T, Boobis AR, Haslett C. Pulmonary fibrosis correlates with duration of tissue neutrophil activation. Am J Respir Crit Care Med 1998; 158: 620-8.
14. 18FFDG labelling of human leukocytes. Nucl Med Commun 2000; 21: 691-4.
15. 18F]-fluoro-D-glucose labelled white blood cells. Mol Imaging Biol 2003; 5: 271-7.
16. 18F] fluorodeoxyglucose labelled leukocytes in normal human subjects. Nucl Med Commun 2002; 23: 721-725.
17. Rini JN, Bhargava KK, Tronco GG, et al. PET with FDG-labeled leukocytes versus scintigraphy with 111In-Oxine-labeled leukocytes for detection of infection. Radiology 2006; 238(3): 978-987.
18. Dumarey N, Egrise D, Blocklet D, et al. Imaging infection with 18F-FDG-labeled leukocytes PET/CT: initial experience in 21 patients. J Nucl Med 2006; 47: 625-32.
19. 18F-FDG. J Nucl Med 2005; 46: 1522-30.
20. Sigma Catalog. Use of trypan blue stain and the hemocytometer to determine viable cell number. 1992; Available at: www.sigmaaldr iche.com
21. Nelson RD, Quie PG, Simmons RL. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol 1975; 115 (6): 1650-6.
22. Smith GS, Limsden JH, Wilcock BP. Chemotaxis of Porcine neutrophils under agarose. Can J Comp Med 1985; 49: 43-9.
23. Kälicke T, Schmitz A, Risse JH, et al. Fluorine-18 fluorodeoxyglucose PET in infectious bone diseases: results of histologically confirmed cases. Eur J Nucl Med 2000; 27(5): 524-8.
24. McAfee JG, Subramanian G, Gagne G. Present trends and future directions in leukocyte labeling, In: Thakur ML, Ed. Radiolabeled cellular blood elements: pathophysiology, techniques and scintigraphic applications. Plenum Press: New York 1983.
25. Warren S. Trypan blue exclusion test of cell viability. Curr Protoc Immunol 1997; A.3B.1-A.3B.2
26. Kwok AKH, Yeung CK, Lai TYY, Chan KP, Pang CP. Effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells. Br J Ophthalmol 2004; 88: 1590-4.
27. Lane TA, Beutler E, West C, Lamkin G. Glycolytic enzymes of stored granulocytes. Transfusion 1984; 24: 153-6.
28. Rijksen G, Staal GEJ, Beks PJ, Streefkerk, Akkerman JWN. Compartmentation of hexokinase in human blood cells characterization of soluble and particulate enzymes. Acta Biochim Biophys 1982; 719: 431-7.
29. Pellegrino D, Bonab AA, Dragotakes SC, Pitman JT, Mariani G, Carter EA. Inflammation and infection: imaging properties of 18FFDG- labeled white blood cells versus 18F-FDG. J Nucl Med 2005; 46: 1522-30.
30. Rini JN, Palestro CJ. Imaging of infection and inflammation with 18F-FDG-labeled leukocytes. Q J Nucl Med Mol Imaging 2006; 50: 143-6.