Immobilized trypsin on epoxy organic monoliths with modulated hydrophilicity: Novel bioreactors useful for protein analysis by liquid chromatography coupled to tandem mass spectrometry

Journal of Chromatography A

Volumn 1218, Issue 49, 2011, Pages 8937-8945

  Calleri, E ^a   Temporini, C ^a   Gasparrini, F ^b   Simone, P ^b   Villani, C ^b   Ciogli, A ^b   Massolini, G ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Hydrophilicity modulated monoliths; Immobilized trypsin; Kinetic studies; Liquid chromatography tandem mass spectrometry; Micro IMERs; Protein digestion

Indexed keywords

HYDROPHILICITY-MODULATED MONOLITHS; IMMOBILIZED TRYPSIN; KINETIC STUDIES; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; MICRO-IMERS; PROTEIN DIGESTION;

ACRYLIC MONOMERS; AMIDES; AMINO ACIDS; BIOREACTORS; CASEIN; COLUMN CHROMATOGRAPHY; ENZYME IMMOBILIZATION; HIGH PRESSURE LIQUID CHROMATOGRAPHY; HYDROPHILICITY; LIQUIDS; MASS SPECTROMETRY; MONOLITHIC INTEGRATED CIRCUITS; MONOMERS; POLYMERS; RATE CONSTANTS;

PROTEINS;

BETA CASEIN; EPOXY ORGANIC MONOLITH; HUMAN SERUM ALBUMIN; POLYMER; RIBONUCLEASE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; BIOREACTOR; CONTROLLED STUDY; ENZYME IMMOBILIZATION; HYDROPHILICITY; LIPOPHILICITY; LIQUID CHROMATOGRAPHY; MICHAELIS CONSTANT; MOLECULAR WEIGHT; PARTICLE SIZE; POROSITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN POLYMERIZATION; ROOM TEMPERATURE; SURFACE PROPERTY; SYNTHESIS; TANDEM MASS SPECTROMETRY;

ANIMALS; BIOREACTORS; CATTLE; CHROMATOGRAPHY, LIQUID; ENZYMES, IMMOBILIZED; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; METHACRYLATES; MICROSCOPY, ELECTRON, SCANNING; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PROTEINS; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 82955163251     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.05.059     Document Type: Article

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