Phosphorylation of NuSAP by Cdk1 regulates its interaction with microtubules in mitosis

Cell Cycle

Volumn 10, Issue 23, 2011, Pages 4083-4089

  Chou, Han Yi ^a   Wang, Ting Huang ^a,b   Lee, Sheng Chung ^a   Hsu, Pang Hung ^c   Tsai, Ming Daw ^c   Chang, Chih Ning ^a,b   Jeng, Yung Ming ^a,b  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

^c NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

Cdk1; Microtubule; Mitosis; NuSAP

Indexed keywords

CYCLIN DEPENDENT KINASE 1; MICROTUBULE PROTEIN; NUCLEOLAR AND SPINDLE ASSOCIATED PROTEIN; THREONINE; UNCLASSIFIED DRUG;

ANAPHASE; ARTICLE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE PROGRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; METAPHASE; MITOSIS; PROMETAPHASE; PROTEIN BINDING; PROTEIN DEPHOSPHORYLATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION;

EID: 82855169517     PISSN: 15384101     EISSN: 15514005     Source Type: Journal    
DOI: 10.4161/cc.10.23.18200     Document Type: Article

References (20)

1. Wittmann T, Hyman A, Desai A. The spindle: a dynamic assembly of microtubules and motors. Nat Cell Biol 2001; 3:28-34; PMID:11146647; DOI:10.1038/35050669.
2. Rusan NM, Fagerstrom CJ, Yvon AM, Wadsworth P. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein- alpha tubulin. Mol Biol Cell 2001; 12:971-80; PMID:11294900.
3. D'Avino PP, Savoian MS, Glover DM. Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy. J Cell Sci 2005; 118:1549-58; PMID:15811947; DOI:10.1242/jcs.02335.
4. Khmelinskii A, Scheibel E. Assembling the spindle midzone in the right place at the right time. Cell Cycle 2008; 7:283-6; PMID:18235228; DOI:10.4161/cc.7.3.5349.
5. Kline-Smith SL, Walczak CE. Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics. Mol Cell 2004; 15:317-27; PMID:15304213; DOI:10.1016/j.molcel.2004.07.012.
6. Sharp DJ, Rogers GC, Scholey JM. Microtubule motors in mitosis. Nature 2000; 407:41-7; PMID:10993066; DOI:10.1038/35024000.
7. Thein KH, Kleylein-Sohn J, Nigg EA, Gruneberg U. Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity. J Cell Biol 2007; 178:345-54; PMID:17664331; DOI:10.1083/ jcb.200701163.
8. Wong J, Fang G. HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture. J Cell Biol 2006; 173:879-91; PMID:16769820; DOI:10.1083/jcb.200511132.
9. Wittmann T, Wilm M, Karsenti E, Vernos I. TPX2, A novel xenopus MAP involved in spindle pole organization. J Cell Biol 2000; 149:1405-18; PMID:10871281; DOI:10.1083/jcb.149.7.1405.
10. Charrasse S, Schroeder M, Gauthier-Rouviere C, Ango F, Cassimeris L, Gard DL, et al. The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215. J Cell Sci 1998; 111:1371-83; PMID:9570755.
11. Raemaekers T, Ribbeck K, Beaudouin J, Annaert W, Van Camp M, Stockmans I, et al. NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization. J Cell Biol 2003; 162:1017-29; PMID:12963707; DOI:10.1083/jcb.200302129.
12. John PC, Mews M, Moore R. Cyclin/Cdk complexes: their involvement in cell cycle progression and mitotic division. Protoplasma 2001; 216:119-42; PMID:11732181; DOI:10.1007/BF02673865.
13. Chang W, Gruber D, Chari S, Kitazawa H, Hamazumi Y, Hisanaga S, et al. Phosphorylation of MAP4 affects microtubule properties and cell cycle progression. J Cell Sci 2001; 114:2879-87; PMID:11683421.
14. Fu C, Yan F, Wu F, Wu Q, Whittaker J, Hu H, et al. Mitotic phosphorylation of PRC1 at Thr470 is required for PRC1 oligomerization and proper central spindle organization. Cell Res 2007; 17:449-57; PMID:17438553.
15. Vasquez RJ, Gard DL, Cassimeris L. Phosphorylation by CDK1 regulates XMAP215 function in vitro. Cell Motil Cytoskeleton 1999; 43:310-21; PMID:10423272; DOI:10.1002/(SICI)1097- 0169(1999)43:4〈310::AID-CM4〉3. 0.CO;2-J.
16. Ookata K, Hisanaga S, Bulinski JC, Murofushi H, Aizawa H, Itoh TJ, et al. Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J Cell Biol 1995; 128:849-62; PMID:7876309; DOI:10.1083/ jcb.128.5.849.
17. Nigg EA. The substrates of the cdc2 kinase. Semin Cell Biol 1991; 2:261-70; PMID:1842344.
18. Wolf F, Sigl R, Geley S. 'The end of the beginning': Cdk1 thresholds and exit from mitosis. Cell Cycle 2007; 6:1408-11; PMID:17581279; DOI:10.4161/ cc.6.12.4361.
19. Li L, Zhou Y, Sun L, Xing G, Tian C, Sun J, et al. NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity. Cell Signal 2007; 19:2046-55; PMID:17618083; DOI:10.1016/j.cellsig. 2007.05.017.
20. Fang CY, Chen HY, Wang M, Chen PL, Chang CF, Chen LS, et al. Global analysis of modifications of the human BK virus structural proteins by LC-MS/ MS. Virology 2010; 402:164-76; PMID:20381826; DOI:10.1016/j.virol.2010.03.029.