Dried culture spots for Xpert MTB/RIF external quality assessment: Results of a phase 1 pilot study in South Africa

Journal of Clinical Microbiology

Volumn 49, Issue 12, 2011, Pages 4356-4360

  Scott, L E ^a   Gous, N ^a   Cunningham, B E ^a   Kana, B D ^b   Perovic, O ^b   Erasmus, L ^c   Coetzee, G J ^c   Koornhof, H ^c   Stevens, W ^a,d  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^b UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^c National TB Reference Laboratory (NTBRL)   (South Africa)

^d NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

RIFAMPICIN;

ANALYTICAL ERROR; ANTIBIOTIC RESISTANCE; ARTICLE; ASSAY; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CULTURE TECHNIQUE; DRIED CULTURE SPOT; FLOW CYTOMETRY; MYCOBACTERIUM TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS RIFAMPIN RESISTANCE ASSAY; NONHUMAN; PILOT STUDY; PRIORITY JOURNAL; QUALITY CONTROL; SOUTH AFRICA;

DIAGNOSTIC ERRORS; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS; PILOT PROJECTS; QUALITY ASSURANCE, HEALTH CARE; REFERENCE STANDARDS; SOUTH AFRICA; TUBERCULOSIS;

EID: 82455220460     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.05167-11     Document Type: Article

References (28)

1. Armand, S., P. Vanhuls, G. Delcroix, R. Courcol, and N. Lemaitre. 2011. Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J. Clin. Microbiol. 49:1772-1776.
2. Blackwood, K. S., et al. 2005. Viability testing of material derived from Mycobacterium tuberculosis prior to removal from a containment level-III laboratory as part of a Laboratory Risk Assessment Program. BMC Infect. Dis. 5:4.
3. Blakemore, R., et al. 2010. Evaluation of the analytical performance of the Xpert MTB/RIF assay. J. Clin. Microbiol. 48:2495-2501.
4. Boehme, C. C., et al. Rapid molecular detection of tuberculosis and rifampin resistance. N. Engl. J. Med. 363:1005-1015.
5. Boehme, C. C., et al. 2011. Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study. Lancet 377:1495-1505.
6. Glencross, D. K., H. M. Aggett, W. S. Stevens, and F. Mandy. 2008. African regional external quality assessment for CD4 T-cell enumeration: development, outcomes, and performance of laboratories. Cytometry B Clin. Cytom. 74(Suppl. 1):S69-S79. (Pubitemid 351569274)
7. Guio, H., et al. 2006. Method for efficient storage and transportation of sputum specimens for molecular testing of tuberculosis. Int. J. Tuberc. Lung Dis. 10:906-910. (Pubitemid 44197852)
8. Helb, D., et al. 2009. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48:229-237.
9. Hillemann, D., S. Rusch-Gerdes, C. Boehme, and E. Richter. 2011. Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system. J. Clin. Microbiol. 49:1202-1205.
10. Holm, C., T. Mathiasen, and L. Jespersen. 2004. A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk. J. Appl. Microbiol. 97:935-941. (Pubitemid 39390220)
11. Kana, B. D., et al. 2008. The resuscitation-promoting factors of Mycobacterium tuberculosis are required for virulence and resuscitation from dormancy but are collectively dispensable for growth in vitro. Mol. Microbiol. 67:672-684.
12. Malbruny, B., G. Le Marrec, K. Courageux, R. Leclercq, and V. Cattoir. Rapid and efficient detection of Mycobacterium tuberculosis in respiratory and non-respiratory samples. Int. J. Tuberc. Lung Dis. 15:553-555.
13. Marlowe, E. M., et al. Evaluation of the Cepheid Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis complex in respiratory specimens. J. Clin. Microbiol. 49:1621-1623.
14. Miotto, P., F. Piana, G. B. Migliori, and D. M. Cirillo. 2008. Evaluation of the GenoCard as a tool for transport and storage of samples for tuberculosis molecular drug susceptibility testing. New Microbiol. 31:147-150. (Pubitemid 351422085)
15. Moure, R., et al. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method. J. Clin. Microbiol. 49:1137-1139.
16. Norden, M. A., T. A. Kurzynski, S. E. Bownds, S. M. Callister, and R. F. Schell. 1995. Rapid susceptibility testing of Mycobacterium tuberculosis (H37Ra) by flow cytometry. J. Clin. Microbiol. 33:1231-1237.
17. Parsons, L. M., et al. Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities. Clin. Microbiol. Rev. 24:314-350.
18. Pina-Vaz, C., S. Costa-de-Oliveira, and A. G. Rodrigues. 2005. Safe susceptibility testing of Mycobacterium tuberculosis by flow cytometry with the fluorescent nucleic acid stain SYTO 16. J. Med. Microbiol. 54:77-81.
19. Rachow, A. 2010. Detection of Mycobacterium tuberculosis using the Cepheid Xpert MTB/RIF assay: a clinical validation study from Tanzania. 41st Union World Conference on Lung Health, Berlin, Germany.
20. Reis, R. S., I. Neves, Jr., S. L. Lourenco, L. S. Fonseca, and M. C. Lourenco. 2004. Comparison of flow cytometric and Alamar Blue tests with the proportional method for testing susceptibility of Mycobacterium tuberculosis to rifampin and isoniazid. J. Clin. Microbiol. 42:2247-2248. (Pubitemid 38637855)
21. Sakamoto, C., N. Yamaguchi, and M. Nasu. 2005. Rapid and simple quantification of bacterial cells by using a microfluidic device. Appl. Environ. Microbiol. 71:1117-1121. (Pubitemid 40223607)
22. Schellenberg, J., T. Blake Ball, M. Lane, M. Cheang, and F. Plummer. 2008. Flow cytometric quantification of bacteria in vaginal swab samples self-collected by adolescents attending a gynecology clinic. J. Microbiol. Methods 73:216-226.
23. Soejima, T., K. Iida, T. Qin, H. Taniai, and S. Yoshida. 2009. Discrimination of live, anti-tuberculosis agent-injured, and dead Mycobacterium tuberculosis using flow cytometry. FEMS Microbiol. Lett. 294:74-81.
24. Van Deun, A., et al. 2009. Mycobacterium tuberculosis strains with highly discordant rifampin susceptibility test results. J. Clin. Microbiol. 47:3501-3506.
25. Van Rie, A., L. Page-Shipp, L. Scott, I. Sanne, and W. Stevens. 2010. Xpert MTB/RIF for point-of-care diagnosis of TB in high-HIV burden, resourcelimited countries: hype or hope? Expert Rev. Mol. Diagn. 10:937-946.
26. WHO. March 2011. Rapid implementation of the Xpert MTB/RIF diagnostic test. Technical and operation 'how-to' practical considerations. WHO, StopTB. WHO, Geneva, Switzerland. http://www.stoptb.org.
27. WHO. 2004. Laboratory biosafety manual, 3rd ed. HO/CDS/CSR/LYO/2004.11. WHO Geneva, Switzerland. http://www.who.int/csr/resources/publications/ biosafety/Biosafety7.pdf.
28. WHO. 8 December 2010, posting date. WHO endorses new rapid tuberculosis test. WHO, Geneva, Switzerland. http://www.who.int/mediacentre/news/releases/ 2010/tb-test-20101208/en/index.html.