Elderberry (Sambucus nigra) bark contains two structurally different Neu5Ac(α2,6)Gal/GalNAc-binding type 2 ribosome-inactivating proteins

European Journal of Biochemistry

Volumn 245, Issue 3, 1997, Pages 648-655

  Van Damme, Els J M ^a   Roy, Soma ^a   Barre, Annick ^b   Citores, Lucia ^c   Mostafapous, Kazem ^d   Rougé, Pierre ^b   Van Leuven, Fred ^a   Girbés, Tomas ^c   Goldstein, Irwin J ^d   Peumans, Willy J ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITY OF VALLADOLID   (Spain)

^d UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

elderberry; lectin; ribosome inactivating protein; Sambucus nigra

Indexed keywords

RIBOSOME INACTIVATING PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN RNA BINDING; STRUCTURE ANALYSIS;

SAMBUCUS NIGRA;

EID: 8244246430     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00648.x     Document Type: Article

References (27)

1. Barbieri, L., Battelli, M. G. & Stirpe, F. (1993) Ribosome-inactivating proteins from plants, Biochim. Biophys. Acta 1154, 237-282.
2. Bourne, Y., Mazurier, J., Legrand, D., Rougé, P., Montreuil, J., Spik, G. & Cambillau, C. (1994) Interaction of a legume lectin with the human lactotransferrin N2 fragment or with the isolated biantennary glycopeptide: role of the fucose moiety, Structure 2, 209-219.
3. Chaddock, J. A. & Roberts, L. M. (1993) Mutagenesis and kinetic analysis of the active site Glu177 of ricin-A chain, Protein Eng. 6, 425-431.
4. Citores, L., Iglesias, R., Munoz, R., Ferreras, J. M., Jiminez, P. & Girbes, T. (1994) Elderberry (Sambucus nigra L.) seed proteins inhibit protein synthesis and display strong immunoreactivity with rabbit polyclonal antibodies against the type 2 ribosome-inactivating protein nigrin b, J. Exp. Bot. 45, 513-516.
5. Citores, L., de Benito, F. M., Iglesias, R., Ferreras, J. M., Jiminez, P., Argüeso, P., Farias, G., Mendez, E. & Girbes, T. (1996) Isolation and characterization of a new non-toxic two-chain ribosome-inactivating protein from fruits of elder (Sambucus nigra L.), J. Exp. Bot. 47, 1577-1585.
6. Endo, Y., Tsurgi, K. & Lambert, J. M. (1988) The site of action of six different ribosome-inactivating proteins from plants on eukaryotic ribosomes: the RNA N-glycosidase activity of the proteins, Biochim. Biophys. Res. Commun. 150, 1032-1036.
7. Gaboriaud, C., Bissery, V., Benchetrit, T. & Mornon, J. P. (1987) Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences, FEBS Lett. 224, 149-155.
8. Girbes, T., Citores, L., Ferreras, J. M., Rojo, M. A., Iglesias, R., Munoz, R., Arias, F. J., Calonge, M., Garcia, J. R. & Mendez, E. (1993a) Isolation and partial characterization of nigrin b, a non-toxic novel type 2 ribosome-inactivating protein from the bark of Sambucus nigra L., Plant Mol. Biol. 22, 1181-1186.
9. Girbes, T., Citores, L., Iglesias, R., Ferreras, J. M., Munoz, R., Rojo, M. A., Arias, F. J., Garcia, J. R., Mendez, E. & Calonge, M. (1993b) Ebulin 1, a nontoxic novel type 2 ribosome-inactivating protein from Sambucus ebulus L. leaves, J. Biol. Chem. 268, 18195-18199.
10. Girbes, T., Citores, L., de Benito, F. M., Iglesias, R. & Ferreras, J. M. (1996) A non-toxic two-chain ribosome-inactivating protein co-exists with a structure-related monomeric lectin (SNA III) in elder (Sambucus nigra) fruits, Biochem. J. 315, 343-344.
11. Kaku, H., Tanaka, Y., Tazaki, K., Minami, E., Mizuno, H. & Shibuya, N. (1996) Isolation and characterization of a second lectin (SNA II) present in elderberry (Sambucus nigra L.) bark, J. Biol. Chem. 271, 1480-1485.
12. Katzin, B. J., Collins, E. J. & Robertus, J. D. (1991) Structure of ricin-A chain at 2.5 Å, Proteins 10, 251-259.
13. Kim, Y. & Robertus, J. D. (1992) Analysis of several key active site residues of ricin A chain by mutagenesis and X-ray crystallography, Protein Eng. 5, 775-779.
14. Kumar, A. M., Timm, D. E., Neet, K. E., Owen, W. G., Peumans, W. J. & Rao, A. J. (1993) Characterization of the lectin from the bulbs of Eranthis hyemalis (winter aconite) as an inhibitor of protein synthesis, J. Biol. Chem. 268, 25176-25183.
15. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
16. Lemesle-Varloot, L., Henrissat, B., Gaboriaud, C., Bissery, V., Morgat, A. & Mornon, J. P. (1990) Hydrophobic cluster analysis: procedure to derive structural and functional information from 2-D-representation of protein sequences, Biochimie 72, 555-574.
17. Ling, J., Liu, W. & Wang, T. P. (1995) Simultaneous existence of two typres of ribosome-inactivating proteins in the seeds of Cinnamonum camphora - characterization of the enzymatic activities of these cytotoxic proteins, Biochim. Biophys. Acta 1252, 15-22.
18. Mierendorf, R. C. & Pfeffer, D. (1987) Direct sequencing of denatured plasmid DNA, Methods Enzymol. 152, 556-562.
19. Pelham, H. D. B. & Jackson, R. J. (1976) An efficient mRNA-dependent translation system from reticulocyte lysates, Eur. J. Biochem. 67, 247-256.
20. Peumans, W. J. & Van Damme, E. J. M. (1995) Lectins as plant defense proteins, Plant Physiol. 109, 347-352.
21. Rutenber, E., Katzin, B. J., Collins, E. J., Mlsna, D., Ready, M. P. & Robertus, J. D. (1991) Crystallographic refinement of ricin to 2.5 Å, Proteins 10, 240-250.
22. Rutenber, E. & Robertus, J. D. (1991) Structure of the ricin B-chain at 2.5 Å resolution, Proteins 10, 260-269.
23. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) DNA sequencing with chain terminating inhibitors, Proc. Natl Acad. Sci. USA 74, 5463-5467.
24. Van Damme, E. J. M., Kaku, H., Perini, F., Goldstein, I. J., Peeters, B., Yagi, F., Decock, B. & Peumans, W. J. (1991) Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin, Eur. J. Biochem. 202, 23-30.
25. Van Damme, E. J. M., Barre, A., Rougé, P., Van Leuven, F. & Peumans, W. J. (1996a) Characterization and molecular cloning of SNAV (nigrin b), a GalNAc-specific type 2 ribosome-inactivating protein from the bark of elderberry (Sambucus nigra), Eur. J. Biochem. 235, 505-513.
26. Van Damme, E. J. M., Barre, A., Rougé, P., Van Leuven, F. & Peumans, W. J. (1996b) The NeuAc(α2,6)Gal/GalNAc binding lectin from elderberry (Sambucus nigra) bark is a type 2 ribosome inactivating protein with an unusual specificity and structure, Eur. J. Biochem. 235, 128-137.
27. Van Damme, E. J. M., Barre, A., Rougé, P., Van Leuven, F. & Peumans, W. J. (1997) Isolation and molecular cloning of a novel type 2 ribosome-inactivating protein with an inactive B chain from elderberry (Sambucus nigra) bark, J. Biol. Chem. 272, in the press.