Utilization of an in vitro assay to evaluate chromatin degradation by candidate apoptotic nucleases

Cell Death and Differentiation

Volumn 4, Issue 3, 1997, Pages 200-208

  Hughes Jr , Francis M ^a   Cidlowski, John A ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

Apoptosis; Cell death; Endonuclease; Nuclease assay

Indexed keywords

CYCLOPHILIN; DEOXYRIBONUCLEASE I; DEOXYRIBONUCLEASE II; DNA FRAGMENT; GLUCOCORTICOID; NUCLEASE;

APOPTOSIS; ARTICLE; CATABOLISM; CHROMATIN; CHROMATIN STRUCTURE; CONTROLLED STUDY; DNA CLEAVAGE; DNA DEGRADATION; HELA CELL; HUMAN; HUMAN CELL; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION;

BACTERIA (MICROORGANISMS);

EID: 8244231274     PISSN: 13509047     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cdd.4400221     Document Type: Article

References (49)

1. Arends MJ, Morns RG and Wyllie AH (1990) Apoptosis: The role of the endonuc lease Am J Pathol 136: 593 - 608
2. Barry MA and Eastman A (1993) Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch Biochem Biophys. 300: 440 - 450
3. Beere HM, Chresta CM, Alejoherberg A, Skladanowstci A, Dive C, Larsen AK and Hickman JA (1995) Investigation of the mechanism of higher order chromatin fragmentation observed in drug-induced apoptosis. Mol Pharmacol. 47: 986 - 996
4. Bicknell GR and Cohen GM (1995) Cleavage of DNA to large kilobase pair fragments occurs in some forms of necrosis as well as apoptosis. Biochem Biophys Res Commun 207: 40 - 47
5. Birren B and Lai E (1993) Pulsed Field Gel Electrophoresis: A Pratical Guide. San Diego, CA: Academic Press, Inc., p
6. Boone DL, Yan W and Tsang BK (1995) Identification of a deoxyribonuclease l-like endonuclease in rat granulosa and luteal cell nuclei. Biol Reprod. 53: 1057 - 1065
7. Bortner CD Oldenburg NBE and Cidlowski JA (1995) The role of DNA fragmentation in apoptosis. Trends Cell Biol. 5: 21 - 26
8. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248 - 254
9. Brown DG, Sun X-M and Cohen GM (1993) Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation. J Biol Chem. 268: 3037 - 3039
10. Cain KS, Inayat-Hussain H, Kokileva L and Cohen GM (1995) Multi-step DNA cleavage in rat liver nuclei is inhibited by thiol reactive agents FEBS Lett. 358: 255 - 261
11. 2+ alone FEES Lett 349: 385 - 391
12. Cidlowski JA (1982) Glucocorticoids stimulate riboriucletc acid degradation in isolated rat thymic lymphocytes in vitro. Endocrinology. 111: 184 - 190
13. Cohen GM, Sun X-M, Fearnhead H, MacFarlane M, Brown DG, SRT and Dinsdale D (1994) Formation of large molecular weight fragments of DNA is a key committed step of apoptosis in thymocytes. J Immunol 153: 507 - 516
14. Cohen JJ and Duke RC (1984) Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cel death. J Immunol. 132: 38 - 42
15. Compton MM and Cidlowski JA (1986) Rapid in vivo effects of glucocorticoids on the integrity of rat lymphocyte genomic deoxyribonucleic acid. Endocrinology. 118: 38 - 45
16. Eastman A (1994) Deoxyribonuclease II in apoptosis and the significance of intracellular acidification Cell Death Differ, 1: 7 - 10
17. Filipski J, Leblance J, Youdale T, Sikorska M and Walker PR (1990) Periodicity of DMA folding in higher order chromatin structures EMBO J. 9: 1319 - 1327
18. 2B. J Biol Chem. 266: 18580 - 18585
19. Gromova II, Nielsen OF and Razin SV (1995) Long-range fragmentation of the eukaryotic genome by exogenous and endogenous nucleases proceeds in a specific fashion via preferential DNA cleavage at matrix attachment sites. J Biol Chem 270: 18685 - 18690
20. Hacker G and Vaux DL (1995) The medical significance of physiological cell death. Med Res Rev 15: 299 - 311
21. Hewish DR and Burgoyne LA (1973) Chromatin substructure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease Biochem Biophys Res Commun. 52: 504 - 510
22. Hughes FM Jr and Cidlowski JA (1994) Apoptotic DMA Degradation: Evidence for Novel Enzymes. Cell Death Differ. 1. 11 - 17
23. Kataoka A, Kubota M, Wakazono Y, Okuda A, Bessho R, Lin YW, Usami I, Akiyama Y and Furusho K (1995) Association of high molecular weight DNA fragmentation with apoptotic or non-apoptotic cell death induced by calcium ionophore. FEBS Lett. 364. 264 - 267
24. Kokileva L (1994) Multi-step chromatin degradation in apoptosis Int Arch Allergy Immunol 105 339 - 343
25. Kokileva L (1995) Comparative study of induction of endogenous DN A degradation in rat liver nuclei and intact thymocyles Comp Biochem Physiol Biochem Mol Biol. 111: 35 - 43
26. Kokileva LB (1979) DNA degradation in isolated rat liver nuclei. Molec Biol Rep 5: 171 - 174
27. Laemmli UK, Kass E, Polijak E and Adachi Y (1992) Scaffold associated regions: cisacting determinants of chromatin structural loops and functional domains. Curr Opin Genet Dev. 2: 275 - 285
28. Lazebnik YA, Cole S, Cooke CA, Nelson WG and Eamshaw WC (1993) Nuclear events of apoptosis in vitro in cell-free mitotc extracts: a model system for analysis of the active phase of apoptosis. J Cell Biol. 123: 7 - 22
29. McConkey DJ, Hartzell P, Amador-Pérez JF. Orrenius S and Jondal M (1989a) Calcium-dependent killing of immature thymocytes by stimulation via the CD3/T cell receptor complex. J Immunol 143: 1801 - 1806
30. McConkey DJ, Hartzell P, Nicotera P and Orrenius S (1989b) Calcium-activated DNA fragmentation kills immature thymocytes. FASEB. 3: 1843 - 1849
31. Mirkovich J, Mirault ME and Laemmli UK (1984) Organization of higher order chromatin loop-domain' specific DMA attachment sites on the nuclear scaffold. Cell. 39. 223 - 232
32. Montague JW. Gaido ML, Frye C and Cidlowski JA (1994) A Calcium-dependent nuclease from apoptotic rat thymocytes is homologous with cyclophilin: Recombinant cyclophilms A and B exhibit nuclease activity J Biol Chem. 269: 18877 - 18880
33. Montague JW, Hughes FM Jr and Cidlowski JA (1996) Purified recombinant cyclophilins A, B, and C facilitate nucleic acid degradation independently of peptidylprolyl cis-trans isomerase activity: potential roles in apoptosis. J Biol Chem (in press)
34. Nieto MA and Lopez-Rivas A (1989) IL-2 protects T lymphocytes from glucocorticoidinduced DNA fragmentation and cell death J Immunol. 143: 4166 - 4170
35. Oberhammer F, Wilson JW, Dive C, Morris ID, Hickman JA, Wakeling AE, Walker PR and Sikorska M (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12: 3679 - 3684
36. Ormerod MG, O'Neill CF, Robertson D and Harrap KR (1994) Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA Exp Cell Res. 211: 231 - 237
37. Peitsch MC, Polzar B, Stephan H, Crompton T, MacDonald HR, Mannherz HG and Tschopp J (1993) Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death). EMBO J. 12: 371 - 377
38. Peitsch MC, Polzar B, Tschopp J and Mannherz HG (1994) About the involvement of deoxyribonuclease I in apoptosis. Cell Death Differ. 1: 1 - 6
39. Saitoh Y and Laemmli UK (1994) Metaphase chromosome structure: bands arise from a differential foiding path of the highly AT-rich scaffold Cell. 76: 609 - 622
40. Schwartzman RA and Cidlowski JA (1991) Internucleosomal deoxyribonuc̀leic acid cleavage activity in apoptotic thymocytes. detection and endocrine regulation. Endocrinology. 128: 1190 - 1197
41. Schwartzman RA and Cidlowski JA (1993a) Apoptosis: The biochemistry and molecular biology of programmed cell death Endocrine Rev 14: 133 - 151
42. Swhwartzman RA and Cidlowski JA (1993b) Mechanism of tissue-specific induction of internucleosomal deoxynbonucleic acid cleavage activity and apoptosis by glucocorticoids. Endocrinology 133: 591 - 599
43. 2+-dependent cleavage of DNA into kilobase pair fragments is responsible for the initial degradation of DNA in apoptosis. J Biol Chem. 269: 14857 - 14860
44. Vanderbilt JN, Bloom KS and Andersen JN (1982) Endogenous nuclease Properties and effects on transcribed genes in chromatin. J Biol Chem. 257: 13009 - 13017
45. Walker PR, Kokileva L, LeBlanc J and Sikorska M (1993) Detection of the initial stages of DNA fragmentation in apoptosis. BioTechniques 15. 1032 - 1040
46. Walker PR, Pandey S and Sikorska M (1995) Degradation of chromatin in apoptotic cells. Cell Death Differ 2: 97 - 104
47. Walker PR and Sikorska M (1986) Modulation of the sensitivity of chromatin to exogenous nucleases implications for the apparent increased sensitivity of transcriptionally active genes. Biochemistry. 25: 3839 - 3845
48. Walker PR, Weaver VM, Lach B, LeBlanc J and Sikorska M (1994) Endonuclease activities associated with high molecular weight and internucleosomal DNA fragmentation in apoptosis. Exp Cell Res. 213: 100 - 106
49. Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555 - 556