Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions

Methods in Molecular Biology

Volumn 803, Issue , 2012, Pages 205-218

  Müller, Mathias Q ^a   Sinz, Andrea ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Chemical cross linking; Isotope labeling; Mass spectrometr; Proteindrug interaction

Indexed keywords

CROSS LINKING REAGENT; DRUG; DRUG DERIVATIVE; ESTER; GW 6471; LIGAND; N HYDROXYSUCCINIMIDE; N-HYDROXYSUCCINIMIDE; OXAZOLE DERIVATIVE; PEPTIDE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PROTEIN; SUCCINIMIDE DERIVATIVE; TYROSINE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; DRUG ANTAGONISM; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NANOTECHNOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; STATISTICS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROSS-LINKING REAGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESTERS; HYDROLYSIS; LIGANDS; MOLECULAR SEQUENCE DATA; NANOTECHNOLOGY; OXAZOLES; PEPTIDES; PHARMACEUTICAL PREPARATIONS; PPAR ALPHA; PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STATISTICS AS TOPIC; SUCCINIMIDES; TYROSINE;

EID: 82355190830     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-364-6_14     Document Type: Article

References (19)

1. Young, M. M., Tang, N., Hempel, J. C., Oshiro, C. M., Taylor, E. W., Kuntz, I. D., et al. (2000) High throughput protein fold identification by using experimental constraints derived from intramolecular cross-links and mass spectrometry. Proc Natl Acad Sci USA. 97, 5802-5806. (Pubitemid 30367479)
2. Sinz, A. (2003) Chemical cross-linking and mass spectrometry for mapping three-dimensional structures of proteins and protein complexes. J Mass Spectrom. 38, 1225-1237. (Pubitemid 38072855)
3. Sinz, A. (2006) Chemical cross-linking and mass spectrometry to map three-dimensional protein structures and protein-protein interactions. Mass Spectrom Rev. 25, 663-682. (Pubitemid 44014105)
4. Lamos, S., Krusemark, C., Mcgee, C., Scalf, M., Smith, L., Belshaw, P. (2006) Mixed isotope photoaffinity reagents for identification of small-molecule targets by mass spectrometry. Angew Chem Int Ed. 45, 4329-4333. (Pubitemid 44105644)
5. Sinz, A. (2006) Isotope-Labeled Photoaffinity Reagents and Mass Spectrometry To Identify Protein-Ligand Interactions. Angew Chem Int Ed. 46, 660-662.
6. Müller, M. Q., de Koning, L. J., Schmidt, A., Ihling, C., Syha, Y., Rau, O., et. al. (2009) An Innovative Method to Study Target Protein-Drug Interactions by Mass Spectrometry. J. Med. Chem. 52, 2875-2879.
7. Michalik, L., Wahli, W. (1999) Peroxisome proliferator-activated receptors: three isotypes for a multitude of functions. Curr Opin Biotechnol. 10, 564-570.
8. Plutzky, J. (2003) The potential role of peroxisome proliferator- activated receptors on inflammation in type 2 diabetes mellitus and atherosclerosis. Am J Cardiol. 92, 34J-41J.
9. Evans, R. M., Barish, G. D., Wang, Y. (2004) PPARs and the complex journey to obesity. Nat Med. 10, 355-361. (Pubitemid 38508511)
10. Issemann, I., Prince, R. A., Tugwood, J. D., Green, S. (1993) The peroxisome proliferatoractivated receptor:retinoid X receptor heterodimer is activated by fatty acids and fibrate hypolipidaemic drugs. J Mol Endocrinol. 11, 37-47. (Pubitemid 23297949)
11. Ihling, C., Schmidt, A., Kalkhof, S., Schulz, D. M., Stingl, C., Mechtler, K., et al. (2006) Isotope-labeled cross-linkers and Fourier transform ion cyclotron resonance mass spectrometry for structural analysis of a protein/peptide complex. J Am Soc Mass Spectrom. 17, 1100-1113. (Pubitemid 44094125)
12. Schmidt, A., Kalkhof, S., Ihling, C., Cooper, D. M. F., Sinz, A. (2005) Mapping protein interfaces by chemical cross-linking and Fourier transform ion cyclotron resonance mass spectrometry: application to a calmodulin/adenylyl cyclase 8 peptide complex. Eur J Mass Spectrom. 11, 525-534. (Pubitemid 41796541)
13. Müller, M. Q., Roth, C., Sträter, N., Sinz, A. (2008) Expression and purification of the ligand-binding domain of peroxisome proliferator-activated receptor alpha (PPARalpha) Protein Expr Purif. 62, 185-189.
14. Xu, H.E., Stanley, T. B., Montana, V. G., Lambert, M. H., Shearer, B. G., Cobb, J. E. et al. (2002) Structural basis for antagonistmediated recruitment of nuclear co-repressors by PPARalpha. Nature. 415, 813-817. (Pubitemid 34150506)
15. Rau, O., Syha, Y., Zettl, H., Kock, M., Bock, A., Schubert-Zsilavecz, M. (2008) alpha-Alkyl Substituted Pirinixic Acid Derivatives as Potent Dual Agonists of the Peroxisome Proliferator Activated Receptor Alpha and Gamma. Arch Pharm. 341, 191-195.
16. Neuhoff, V., Arold, N., Taube, D., Ehrhardt, W. (1988) Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis. 9, 255-262.
17. Peri, S., Steen, H., Pandey, A. (2001) GPMAW - a software tool for analyzing proteins and peptides. Trends Biochem Sci. 26, 687-689.
18. Kalkhof, S., Sinz, A. (2008) Chances and pitfalls of chemical cross-linking with aminereactive N-hydroxysuccinimide esters Anal Bioanal Chem. 392, 305-312.
19. Mädler, S., Bich, C., Touboul, D., Zenobi, R. (2009) Chemical cross-linking with NHS esters: a systematic study on amino acid reactivities. J Mass Spectrom. 44, 694-706.