Nucleotide diversity and gene expression of Catalase and Glutathione peroxidase in irradiated Scots pine (Pinus sylvestris L.) from the Chernobyl exclusion zone

Journal of Environmental Radioactivity

Volumn 106, Issue , 2012, Pages 20-26

  Vornam, Barbara ^a   Arkhipov, Andrey ^b   Finkeldey, Reiner ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b Chernobyl Center   (Ukraine)

Author keywords

Gene expression; Irradiation; Pinus sylvestris; SNPs

Indexed keywords

ADAPTIVE RESPONSE; CHERNOBYL EXCLUSION ZONE; EXPRESSION PROFILE; GENE FRAGMENTS; GLUTATHIONE PEROXIDASE; MOLECULAR BASIS; NUCLEOTIDE DIVERSITY; PINE TREES; PINUS SYLVESTRIS; RADICAL SCAVENGING; REAL-TIME PCR; SCOTS PINE; SNPS; SOMATIC MUTATION;

ACCIDENTS; FORESTRY; IONIZING RADIATION; IRRADIATION; NUCLEOTIDES; PEPTIDES;

GENE EXPRESSION;

ALPHA TUBULIN; CATALASE; GLUTATHIONE PEROXIDASE;

ADAPTATION; CHERNOBYL ACCIDENT; CONIFEROUS TREE; ENZYME; GENE EXPRESSION; GENETIC VARIATION; IRRADIATION; MOLECULAR ANALYSIS; MUTATION; POLYMERASE CHAIN REACTION; RADIATION EXPOSURE; SCAVENGING (CHEMISTRY); TOLERANCE;

ARTICLE; CHERNOBYL ACCIDENT; CONTROLLED STUDY; DOWN REGULATION; ENVIRONMENTAL RADIOACTIVITY; GENE EXPRESSION; GENE SEQUENCE; GENETIC VARIABILITY; IONIZING RADIATION; NONHUMAN; PILOT STUDY; RADIATION EXPOSURE; SCOTS PINE; SINGLE NUCLEOTIDE POLYMORPHISM; SOMATIC MUTATION; UPREGULATION;

CATALASE; CESIUM RADIOISOTOPES; CHERNOBYL NUCLEAR ACCIDENT; GENE EXPRESSION; GLUTATHIONE PEROXIDASE; MUTATION; NUCLEOTIDES; PINUS SYLVESTRIS; PLANT LEAVES; RADIATION MONITORING; RADIATION, IONIZING; STRONTIUM RADIOISOTOPES; TUBULIN;

FORESTRY; GENES; NUCLEOTIDES; PEPTIDES; PINUS SYLVESTRIS; RADIATION EFFECTS;

PINUS SYLVESTRIS;

EID: 82155195576     PISSN: 0265931X     EISSN: 18791700     Source Type: Journal    
DOI: 10.1016/j.jenvrad.2011.11.001     Document Type: Article

References (43)

1. Arkhipov N., Kuchma N., Askbrant S., Pasternak P.S., Musica V.V. Acute and long-term effects of irradiation on pine (Pinus sylvestris) stands post-Chernobyl. Sci. Total Environ. 1994, 157:383-386.
2. Barci-Funel G., Dalmasso J., Barci V.L., Ardisson G. Study of the transfer of radionuclides in trees at a forest site. Sci. Total Environ. 1995, 173/174:369-373.
3. Brown G.R., Gill G.P., Kuntz R.J., Langley C.H., Neale D.B. Nucleotide diversity and linkage disequilibrium in loblolly pine. Proc. Nat. Acad. Sci. 2004, 101:15255-15260.
4. Brown J.E., Alfonso B., Avila R., Beresford N.A., Copplestone D., Pröhl G., Ulanovsky A. The ERICA tool. J. Environ. Radioact. 2008, 99:1371-1383.
5. Boubriak I.I., Grodzinsky D.M., Polischuk V.P., Naumenko V.D., Gushcha N.P., Micheev A.N., McCready S.J., Osborne D.J. Adaptation and impairment of DNA repair function in pollen of Betula verrucosa and seeds of Oenothera biennis from differently radionuclide-contaminated sites of Chernobyl. Ann. Bot. 2008, 101:267-276.
6. Chang S., Puryear J., Cairney J. Asimple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Rep. 1993, 11:113-116.
7. Chernobyl Forum Chernobyl: The True Scale of the Accident. 20Years Later a UN Report Provides Definitive Answers and Ways to Repair Lives 2005, IAEA, WHO, UNDP.
8. Copplestone D., Hingston J., Real A. The development and purpose of the FREDERICA radiation effects database. J. Environ. Radioact. 2008, 99:1456-1463.
9. Dat J., Vandenabeele S., Vranová E., Van Montagu M., Inzé D., Van Breusegem F. Dual action of the active oxygen species during plant stress responses. Cell. Mol. Life Sci. 2000, 57:779-795.
10. Eshdat Y., Holland D., Faltin Z., Ben-Hayyim G. Plant glutathione peroxidases. Physiol. Plant 1997, 100:234-240.
11. Ewing B., Hillier L.D., Wendl M.C., Green P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Gen. Res. 1998, 8:175-185.
12. Geraskin S., Oudalova A., Dikareva N., Mozolin E., Prytkova J., Dikarev V., Chernonog E., Novikova T. Effects of Radioactive Contamination on Plant Populations and Radiation Protection of Biota. Proceedings of Third European IRPA Congress 2010 June 2010, Finland, Helsinki, pp. 14-18.
13. Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nuc. Acids Symp. Ser. 1999, 41:95-98.
14. IAEA International Atomic Energy Agency. Generic Procedures for Monitoring in a Nuclear or Radiological Emergency 1999, IAEA-TECDOC-1092, Vienna.
15. IAEA, 2006. International Atomic Energy Agency. Environmental Consequences of the Chernobyl Accident and their Remediation: Twenty years of experience. Report of the Chernobyl Forum Expert Group 'Environment'. Vienna.
16. Kalchenko V.A., Arkhipov N., Fedotov I.S. Mutagenesis of enzyme loci, induced in megaspores of Pinus sylvestris L. by ionizing radiation from the accident at the Chernobyl Atomic Power Plant. Genetika 1993, 29:266-273.
17. Kalchenko V.A., Fedotov I.S. Genetic effects of acute and chronic ionizing irradiation on Pinus sylvestris L., inhabiting the Chernobyl' meltdown area. Russ. J. Genet. 2001, 37:341-350.
18. Kovalchuk I., Abramov V., Pogribny I., Kovalchuk O. Molecular aspects of plant adaptation to Life in the Chernobyl zone. Plant Physiol. 2004, 135:357-363.
19. Kovalchuk I., Molinier J., Yao Y., Arkhipov A., Kovalchuk O. Transcriptome analysis reveals fundamental differences in plant response to acute and chronic exposure to ionizing radiation. Mut. Res. 2007, 624:101-113.
20. Kuchma O., Vornam B., Finkeldey R. Mutation rates in Scots pine (Pinus sylvestris L.) from the Chernobyl exclusion zone evaluated with AFLP and microsatellite markers. Mutat. Res. Genet. Toxicol. Environ. Mutagen 2011, 725:29-35.
21. 137Cs in the forests of the 30-km zone around the Chernobyl nuclear power plant. Sci. Total Environ. 1997, 193:169-177.
22. Mittler R., Vanderauwera S., Gollery M., Van Breusegem F. Reactive oxygen gene network of plants. Trends Plant Sci. 2004, 9:490-498.
23. Møller A.P., Mousseau A.A. Biological consequences of Chernobyl: 20 years on. Trends Ecol. Evol. 2006, 21:200-207.
24. Neale D.B. Genomics to tree breeding and forest health. Cur. Opi. Gen. Develop. 2007, 17:539-544.
25. Nomenclature Committee of the International Union of Biochemistry Nomenclature for incompletely specified bases in nucleic acid sequences. Recommendations 1984. Biochem. J. 1985, 229:281-286.
26. Pfaffl M.W., Horgan G.W., Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in realtime PCR. Nuc. Acids Res. 2002, 30:e36.
27. Ralph S.G., Chun H.J., Kolosova N., Cooper D., Oddy C., Ritland C.E., Kirkpatrick R., Moore R., Barber S., Holt R.A., Jones S.J., Marra M.A., Douglas C.J., Ritland K., Bohlmann J. Aconifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis). BMC Genomics 2008, 9:484. 10.1186/1471-2164-9-484.
28. Rantavaara A., Calmon P., Wendt J., Vetikko V. Forest Food Chain and Dose Model (FDMF) for RODOS. Model Description 2001, STUK-A178, Helsinki.
29. Rodriguez Milla M.A., Maurer A., Rodriguez Huete A., Gustafson J.P. Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways. Plant J. 2003, 36:602-615.
30. Rozas J., Sanchez-Del Barrio J.C., Messeguer X., Rozas R. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 2003, 19:2496-2497.
31. Rozen, S., Whitehead Institute/MIT Center for Genome Research. Available at: http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi.
32. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Plainview, New York. second ed.
33. Sanger F.S., Nicklen S., Coulson A.R. DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. 1977, 74:5463-5467.
34. Shaw G., Avila R., Fesenko S., Dvornik A., Zhuchenko T. Modelling the behaviour of radiocaesium in forest ecosystems", Modelling Radioactivity in the Environment 2002, Elsevier, Amsterdam. E.M. Scott (Ed.).
35. Schlichting C.D., Smith H. Phenotypic plasticity: linking molecular mechanisms with evolutionary outcomes. Evol. Ecol. 2002, 16:189-211.
36. Teixeira F.K., Menezes-Benavente L., Galvão V.C., Margis-Pinheiro M. Multigene families encode the major enzymes of antioxidant metabolism in Eucalyptus grandis L. Genet. Mol. Biol. 2005, 28:529-538.
37. Thompson J.D., Higgins D.G., Gibson T.J. Clustal-W - improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nuc. Acids Res. 1994, 22:4673-4680.
38. Tikhomirov F.A., Shcheglov A.I. Main investigation results on the forest radioecology in the Kyshtym and Chernobyl accident zones. Sci. Total Environ. 1994, 157:45-57.
39. Ulanovsky A., Prohl G., Gomez-Ros J.M. Methods for calculating dose conversion coefficients for terrestrial and aquatic biota. J. Environ. Radioact. 2008, 99:1440-1448.
40. Vornam B., Kuchma O., Kuchma N., Arkhipov A., Finkeldey R. SSRs are suitable markers to reveal mutation events in Scots pine (Pinus sylvestris L.) from Chernobyl. Eur. J. For. Res. 2004, 123:245-248.
41. Wi S.G., Chung B.Y., Kim J.-S., Kim J.-H., Baek M.-H., Lee J.-W., Kim Y.S. Effects of gamma irradiation on morphological changes and biological responses in plants. Micron 2007, 38:553-564.
42. 6PDH activities in Stipa capillata (Poaceae). J. Exp. Bot. 2002, 53:1979-1987.
43. Zelena L., Sorochinsky B., von Arnold S., van Zyl L., Clapham D.H. Indications of limited altered gene expression in Pinus sylvestris trees from the Chernobyl region. J. Environ. Radioact. 2005, 84:363-373.